Nanoparticle-based assays in automated flow systems: A review

Passos, Marieta L.C.; Pinto, Paula C.A.G.; Santos, João L.M.; Saraiva, M. Lúcia M.F.S.; Araújo, André R. T. S.

doi:10.1016/j.aca.2015.05.052

Cited by 31 publications

(13 citation statements)

References 147 publications

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“…In the field of flow analysis, this can be seen as an increasing interest in the application of two-and three-dimensional nanostructures for the enhancement of detection techniques, in separation processes carried out under flow conditions and in the creation of nanofluidic and microfluidic analytical systems [345]. In the development of new methods for spectral detections, the use of quantum dots proved to be particularly fruitful [346], while, in the development of electrochemical detections and separation methods, other inorganic nanoparticles with different functionalization were used [347]. Then, the development of especially efficient and fast methods for the synthesis of various nanoparticles was the subject of numerous works in the field of flow synthesis.…”

Section: Robotics Digital Transmission and Camera-enabled Techniquementioning

confidence: 99%

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Trojanowicz

2020

Molecules

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Flow chemistry is an area of contemporary chemistry exploiting the hydrodynamic conditions of flowing liquids to provide particular environments for chemical reactions. These particular conditions of enhanced and strictly regulated transport of reagents, improved interface contacts, intensification of heat transfer, and safe operation with hazardous chemicals can be utilized in chemical synthesis, both for mechanization and automation of analytical procedures, and for the investigation of the kinetics of ultrafast reactions. Such methods are developed for more than half a century. In the field of chemical synthesis, they are used mostly in pharmaceutical chemistry for efficient syntheses of small amounts of active substances. In analytical chemistry, flow measuring systems are designed for environmental applications and industrial monitoring, as well as medical and pharmaceutical analysis, providing essential enhancement of the yield of analyses and precision of analytical determinations. The main concept of this review is to show the overlapping of development trends in the design of instrumentation and various ways of the utilization of specificity of chemical operations under flow conditions, especially for synthetic and analytical purposes, with a simultaneous presentation of the still rather limited correspondence between these two main areas of flow chemistry.

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Section: Robotics Digital Transmission and Camera-enabled Techniquementioning

confidence: 99%

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Trojanowicz

2020

Molecules

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“…Metallic nanostructures offer many advantages such as good stability, good sensitivity, low detection limits, and detection potentials. For this reason, they are very suitable to be used in CE . Specifically, metallic nanostructures allow a decrease of the redox potentials, the electrocatalysis in certain analytes such as carbohydrates and amino acids, and besides they avoid fouling typically shown in carbon‐based detectors .…”

Section: Metallic Nanostructures In Ce and Mementioning

confidence: 99%

Electrochemical detectors based on carbon and metallic nanostructures in capillary and microchip electrophoresis

et al. 2016

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Carbon and metallic-based nanostructures have been progressively implemented as innovative electrochemical detectors in CE and microchip electrophoresis (ME). For both type of nanomaterials and toward selected examples, this review details the impact of these nanomaterials for enhanced detection performance in CE, ME, and paper-based microfluidic devices. The analytical performance and the analytical potential in real world applications is also presented and discussed.

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“…These publications gave rise to FIA and with it to an entire field of research that, over the following 3 decades, has involved thousands of researchers, which to date has resulted in more than 16,000 publications in the scientific literature (McKelvie, 2008;Rocha and Zagatto, 2020). This technique is generally based on straightforward and cost-effective manifolds with the possibility of being adapted to distinct analytical requests (Passos et al, 2015). Flow injection immunoassays (FI-IA), combining FIA technique with immunoassay (IA), provide specific detection and allow rapid and reliable determination.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Flow Injection Immunoassay System for Algal Toxins Determination: A Case of Study

et al. 2021

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A novel flow injection microfluidic immunoassay system for continuous monitoring of saxitoxin, a lethal biotoxin, in seawater samples is presented in this article. The system consists of a preimmobilized G protein immunoaffinity column connected in line with a lab-on-chip setup. The detection of saxitoxin in seawater was carried out in two steps: an offline incubation step (competition reaction) performed between the analyte of interest (saxitoxin or Ag, as standard or seawater sample) and a tracer (an enzyme-conjugated antigen or Ag*) toward a specific polyclonal antibody. Then, the mixture was injected through a “loop” of a few μL using a six-way injection valve into a bioreactor, in line with the valve. The bioreactor consisted of a small glass column, manually filled with resin upon which G protein has been immobilized. When the mixture flowed through the bioreactor, all the antibody-antigen complex, formed during the competition step, is retained by the G protein. The tracer molecules that do not interact with the capture antibody and protein G are eluted out of the column, collected, and mixed with an enzymatic substrate directly within the microfluidic chip, via the use of two peristaltic pumps. When Ag* was present, a color change (absorbance variation, ΔAbs) of the solution is detected at a fixed wavelength (655 nm) by an optical chip docking system and registered by a computer. The amount of saxitoxin, present in the sample (or standard), that generates the variation of the intensity of the color, will be directly proportional to the concentration of the analyte in the analyzed solution. Indeed, the absorbance response increased proportionally to the enzymatic product and to the concentration of saxitoxin in the range of 3.5 × 10–7–2 × 10–5 ng ml−1 with a detection limit of 1 × 10–7 ng ml−1 (RSD% 15, S N−1 equal to 3). The immunoanalytical system has been characterized, optimized, and tested with seawater samples. This analytical approach, combined with the transportable and small-sized instrumentation, allows for easy in situ monitoring of marine water contaminations.

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Nanoparticle-based assays in automated flow systems: A review

Cited by 31 publications

References 147 publications

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Electrochemical detectors based on carbon and metallic nanostructures in capillary and microchip electrophoresis

Microfluidic Flow Injection Immunoassay System for Algal Toxins Determination: A Case of Study

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