Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis

Eryılmaz, Merve; Tamer, Uğur; Boyaci, İsmail Hakkı

doi:10.1016/j.talanta.2020.120781

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…Similarly, pyrrolidonyl arylamidase (PYR) functionalized magnetic-gold (Fe-Au) nanocomposites for the detection of Group A Streptococcus pyogenes induces a red color with the addition of 4-(dimethylamino)-cinnamaldehyde (DMACA). Bacterial cells detection was due to its affinity to PYR (substrate) enhancing the production of β-naphthylamine, which upon reaction with DMACA, intensified color change from yellow to red (Eryılmaz et al, 2020). Other studies that have used bio-receptors and chromogenic substrates inducing visual color change for the detection of pathogenic bacteria are summarized in Table 2.…”

Section: Pathogenic Microbesmentioning

confidence: 99%

“…Despite the strict implementation of good hygienic and manufacturing practices in many nations of the world, diseases outbreaks relating to food and water infections caused by pathogenic bacteria remain the leading cause of death. Examples of such include; Escherichia coli (Duan et al, 2020), Salmonella typhi (Yi et al, 2019), Salmonella enteritidis (Alamer et al, 2018), Staphylococcus aureus (Du et al, 2020), Vibrio cholera (Peng and Chen, 2019), Vibrio parahaemolyticus (Sun et al, 2019), Bacillus cereus (Li et al, 2018), Listeria monocytogenes (Zhang L. et al, 2016), Shigella flexneri (Feng et al, 2019), Pseudomonas aeruginosa (Das et al, 2019), Campylobacter jejuni (Alamer et al, 2018), and Group A Streptococcus pyogenes (Eryılmaz et al, 2020). A review by Daramola et al (2020) reported traditional-based (plate culture), immunological-based (ELISA), nucleic acid-based (PCR, Sequencing), and protein-based (MALDI-TOF-MS) methods as veritable techniques for the detection of pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Bio-Receptors Functionalized Nanoparticles: A Resourceful Sensing and Colorimetric Detection Tool for Pathogenic Bacteria and Microbial Biomolecules

Daramola¹,

Omole²,

Akinwale³

et al. 2022

Front. Nanotechnol.

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Pathogenic bacteria and several biomolecules produced by cells and living organisms are common biological components posing a harmful threat to global health. Several studies have devised methods for the detection of varying pathogenic bacteria and biomolecules in different settings such as food, water, soil, among others. Some of the detection studies highlighting target pathogenic bacteria and biomolecules, mechanisms of detection, colorimetric outputs, and detection limits have been summarized in this review. In the last 2 decades, studies have harnessed various nanotechnology-based methods for the detection of pathogenic bacteria and biomolecules with much attention on functionalization techniques. This review considers the detection mechanisms, colorimetric prowess of bio-receptors and compares the reported detection efficiency for some bio-receptor functionalized nanoparticles. Some studies reported visual, rapid, and high-intensity colorimetric detection of pathogenic bacteria and biomolecules at a very low concentration of the analyte. Other studies reported slight colorimetric detection only with a large concentration of an analyte. The effectiveness of bio-receptor functionalized nanoparticles as detection component varies depending on their selectivity, specificity, and the binding interaction exhibited by nanoparticles, bio-receptor, and analytes to form a bio-sensing complex. It is however important to note that the colorimetric properties of some bio-receptor functionalized nanoparticles have shown strong and brilliant potential for real-time and visual-aided diagnostic results, not only to assess food and water quality but also for environmental monitoring of pathogenic bacteria and a wide array of biomolecules.

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Section: Pathogenic Microbesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bio-Receptors Functionalized Nanoparticles: A Resourceful Sensing and Colorimetric Detection Tool for Pathogenic Bacteria and Microbial Biomolecules

Daramola¹,

Omole²,

Akinwale³

et al. 2022

Front. Nanotechnol.

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“…Antibody modified gold coated-magnetic nanoparticles (Ab-Au-MNPs) was extensively studied for the immuno-magnetic separation and colorimetric detection of E. coli and Salmonella choleraesius [94,95]. Complementing antibody-modified nanocomposites (magnetic and gold nanoparticles) accomplished improved sensitive and selective detection of Group A Streptococcus pyogenes [96].…”

Section: Functionalized Bi-metallic Nanoparticlesmentioning

confidence: 99%

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

Daramola¹,

Torimiro²,

Fadare³

et al. 2020

NNR

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Infections acquired from ingesting contaminated food and water poses an adverse effect on public health and safety, thus affecting nations' economy. Technical approaches developed over years have contributed adequately to microbial detection in food and water, yet, unveiling spaces for more improvement on early and rapid detection of pathogens. This review highlights different strategy assessing bio-functionalized inorganic nanoparticles towards the detection of pathogens in food and water samples. Conjugates of several bio-receptors and inorganic nanoparticles showed rapid, real-time, repeatability, and appreciable limit of detection in targeted pathogens. A patent referenced in this study established the biocompatibility of bio-functionalized inorganic nanoparticles mechanism. Unique attributes exhibited by bio-functionalized inorganic nanoparticles showed potential and improvement of the existing bio-sensing pathogen detection methods. Each of the identified strategies described showed a promising pathway accommodating the development of simple, and even the fabrication of low-cost materials for easy detection of bacterial pathogens in food and water products.

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“…Thiramanas et al reported the detection of ETEC and S. aureus using AuNPs coated with polyethyleneimine and chlorophenol red β-D-galactopyranoside (CPRG) as the chromogenic substrate [21]. Several studies also reported the use of monoclonal and polyclonal antibody [22][23][24][25], aptamer [26,27], cysteine [28], urease [29], chitosan [30] as bioreceptors and coating components on gold, silver, magnetic, bi-metallic, and metallic oxide for the detection of food and waterborne bacterial pathogens.…”

Section: Introductionmentioning

confidence: 99%

Affinity capture of escherichia coli pathotypes using poly-L-lysine functionalized silver nanoparticles

Daramola¹,

Torimiro²,

Alayande³

2022

Adv. Nat. Sci: Nanosci. Nanotechnol.

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In this study, optical detection of different pathotypes of diarrhoeagenic Escherichia coli (E. coli) was exploited using biosynthesized silver nanoparticles (AgNPs) poly-coated with Poly-L-lysine hydrobromide (PLL) and monitored at 540 nm wavelength. A colour change from reddish-brown to colourless (with brown precipitates) with different absorbance values was obtained for each pathotype within two hours of interaction. The bio-affinity sensing and selective adhesion of poly-coated AgNPs (PLL-AgNPs) and E. coli strains were best observed in Enteroaggregative E. coli (0.067 ± 0.004) and Shiga toxin E. coli (0.070 ± 0.002). Slight differences in the negatively charged O-antigen present in E. coli pathotypes informed their varying binding affinity with PLL-AgNPs. Diarrhoeagenic E. coli monitoring using PLL-AgNPs and optical sensing method is a promising approach toward preventing the use of unsafe water and improving public health.

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Nanoparticle-assisted pyrrolidonyl arylamidase assay for a culture-free Group A Streptococcus pyogenes detection with image analysis

Cited by 7 publications

References 27 publications

Bio-Receptors Functionalized Nanoparticles: A Resourceful Sensing and Colorimetric Detection Tool for Pathogenic Bacteria and Microbial Biomolecules

Bio-Receptors Functionalized Nanoparticles: A Resourceful Sensing and Colorimetric Detection Tool for Pathogenic Bacteria and Microbial Biomolecules

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

Affinity capture of escherichia coli pathotypes using poly-L-lysine functionalized silver nanoparticles

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