Nanoparticle- and microparticle-based luminescence imaging of chemical species and temperature in aquatic systems: a review

Moßhammer, Maria; Brodersen, Kasper Elgetti; Kühl, Michael; Koren, Klaus

doi:10.1007/s00604-018-3202-y

Cited by 41 publications

(29 citation statements)

References 147 publications

(275 reference statements)

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“…We used recently published protocols for imaging O 2 concentration over complex bioactive surfaces by coating beachrock cross-sections with O 2 -sensitive luminescent sensor nanoparticles ( Koren et al, 2015 ; Koren et al 2016 ) followed by ratiometric luminescence imaging of the coated surface with a DSLR camera (without NIR filter and with a 530 nm long pass filter mounted on the camera objective) during brief excitation with blue LED light (445 nm) Details of the imaging system and image acquisition software ( Larsen et al, 2011 ), nanoparticle fabrication ( Koren et al, 2015 ; Mosshammer et al, 2019 ) and biocompatibility ( Trampe et al, 2018 ) are given elsewhere.…”

Section: Methodsmentioning

confidence: 99%

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Kühl

Trampe

Moßhammer

et al. 2019

Preprint

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Far-red absorbing chlorophylls are constitutively present as Chl d in the 14 cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f and red-15 shifted phycobilins via far-red light photoacclimation in a range of cyanobacteria, which enables 16 them to use near-infrared-radiation (NIR) for oxygenic photosynthesis. While the biochemistry 17 and molecular physiology of Chl f-containing cyanobacteria has been unraveled in culture 18 studies, their ecological significance remains unexplored and no data on their in situ activity 19 exist. With a novel combination of hyperspectral imaging, confocal laser scanning microscopy, 20 and nanoparticle-based O2 imaging, we demonstrate substantial NIR-driven oxygenic 21 photosynthesis by endolithic, Chl f-containing cyanobacteria within natural beachrock biofilms 22 that are widespread on (sub)tropical coastlines. This indicates an important role of NIR-driven 23 oxygenic photosynthesis in primary production of endolithic and other shaded habitats. 24 25 Impact statement: Cyanobacteria with chlorophyll f show substantial near-infrared radiation-26 driven photosynthesis in intertidal habitats. 27 Behrendt L, Brejnrod A, Schliep M, Sørensen SJ, Larkum AWD, Kühl M. 2015. Chlorophyll f-388 driven photosynthesis in a cavernous cyanobacterium. ISME Journal 9:2108-2111. 389 Behrendt L, Trampe EL, Nord NB, Nguyen J, Lonco D, Nyarko A, Dhinojwala A, Hershey OH, 390 Kühl M, Barton H. 2019. Life in the dark: Far-red absorbing cyanobacteria extend photic 391 zones deep into terrestrial caves. Environmental Microbiology

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Section: Methodsmentioning

confidence: 99%

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Kühl

Trampe

Moßhammer

et al. 2019

Preprint

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“…The fields of aquatic and plant biology research, among others, also benefit from 3D FLIM (Moßhammer et al, 2019). For instance, O 2 imaging using various nanoparticles is useful for the study of corals and plantbased inter-species systems such as those that exist in the rhizosphere (Fabricius-Dyg et al, 2012;Brodersen et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Luminescence lifetime imaging of three-dimensional biological objects

Dmitriev

Intes

Barroso

2021

Journal of Cell Science

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A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs and organisms in the context of their complex spatiotemporal biological and molecular features. In particular, the modalities of luminescence lifetime imaging, comprising fluorescence lifetime imaging (FLI) and phosphorescence lifetime imaging microscopy (PLIM), in synergy with Förster resonance energy transfer (FRET) assays, provide a wealth of information. On the application side, the luminescence lifetime of endogenous molecules inside cells and tissues, overexpressed fluorescent protein fusion biosensor constructs or probes delivered externally provide molecular insights at multiple scales into protein–protein interaction networks, cellular metabolism, dynamics of molecular oxygen and hypoxia, physiologically important ions, and other physical and physiological parameters. Luminescence lifetime imaging offers a unique window into the physiological and structural environment of cells and tissues, enabling a new level of functional and molecular analysis in addition to providing 3D spatially resolved and longitudinal measurements that can range from microscopic to macroscopic scale. We provide an overview of luminescence lifetime imaging and summarize key biological applications from cells and tissues to organisms.

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“…Therefore, this imaging method is ideally suited for mapping the O 2 microenvironment of structurally complex samples like the seagrass leaf phyllosphere (e.g. Koren et al ., 2019; Moßhammer et al ., 2019).…”

Section: Methodsmentioning

confidence: 99%

Imaging O₂ dynamics and microenvironments in the seagrass leaf phyllosphere with magnetic optical sensor nanoparticles

et al. 2020

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Eutrophication leads to epiphyte blooms on seagrass leaves that strongly affect plant health, yet the actual mechanisms of such epiphyte-induced plant stress remain poorly understood. We used magnetic optical sensor nanoparticles in combination with luminescence lifetime imaging to map the O 2 concentration and dynamics in the heterogeneous seagrass phyllosphere under changing light conditions. By incorporating magnetite into the sensor nanoparticles, it was possible to image the spatial O 2 distribution under flow over seagrass leaf segments in the presence of a strong magnetic field. Local microniches with low leaf surface O 2 concentrations were found under thick epiphytic biofilms, often leading to anoxic microhabitats in darkness. High irradiance led to O 2 supersaturation across most of the seagrass phyllosphere, whereas leaf microenvironments with reduced O 2 conditions were found under epiphytic biofilms at low irradiance, probably driven by self-shading. Horizontal micro-profiles extracted from the O 2 images revealed pronounced heterogeneities in local O 2 concentration over the base of the epiphytic biofilm, with up to 52% reduction in O 2 concentrations in areas with relatively thick (>2 mm), compared with thin (≤1 mm), epiphyte layers in darkness. We also present evidence of enhanced relative internal O 2 transport within leaves with epiphyte overgrowth, compared with bare seagrass leaves, in light as a result of limited mass transfer across thick outward diffusion pathways. The local availability of O 2 was still markedly reduced in the epiphyte-covered leaves, however. The leaf phyllosphere is thus characterized by a complex microlandscape of O 2 availability that strongly affects microbial processes occurring within the epiphytic biofilm, which may have implications for seagrass health, as anoxic microhabitats have been shown to promote the microbiological production of reduced toxic compounds, such as nitric oxide.

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Nanoparticle- and microparticle-based luminescence imaging of chemical species and temperature in aquatic systems: a review

Cited by 41 publications

References 147 publications

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Luminescence lifetime imaging of three-dimensional biological objects

Imaging O₂ dynamics and microenvironments in the seagrass leaf phyllosphere with magnetic optical sensor nanoparticles

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Nanoparticle- and microparticle-based luminescence imaging of chemical species and temperature in aquatic systems: a review

Cited by 41 publications

References 147 publications

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Substantial near-infrared radiation-driven photosynthesis of chlorophyllf-containing cyanobacteria in a natural habitat

Luminescence lifetime imaging of three-dimensional biological objects

Imaging O2 dynamics and microenvironments in the seagrass leaf phyllosphere with magnetic optical sensor nanoparticles

Contact Info

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Imaging O₂ dynamics and microenvironments in the seagrass leaf phyllosphere with magnetic optical sensor nanoparticles