Nanoneedle Transistor-Based Sensors for the Selective Detection of Intracellular Calcium Ions

Son, Donghee; In, Insik; Kim, Byeong-Ju; Koh, Jun Tae; Kim, Tae Hyun; An, Sangmin; Jang, Doyoung; Kim, Gyu Tae; Jhe, Wonho; Hong, Seunghun

doi:10.1021/nn200262u

Cited by 53 publications

(37 citation statements)

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“…( e ) Schematic diagram depicting the intracellular calcium ion sensing experiment using nanoneedle shape transistor-based sensor (NTS). Fluo-4-AM, a fluorescent calcium indicator, was immobilized on the CNT channel of an NTS for the detection of Ca 2+ [15]. ( f ) Optical image of an NTS impenetrated into a HeLa cell [15].…”

Section: Sensingmentioning

confidence: 99%

“…Fluo-4-AM, a fluorescent calcium indicator, was immobilized on the CNT channel of an NTS for the detection of Ca 2+ [15]. ( f ) Optical image of an NTS impenetrated into a HeLa cell [15]. ( g ) Fluorescence image of the NTS after adding 2 μM ionomycin into the extracellular medium [15].…”

Section: Sensingmentioning

confidence: 99%

“…( f ) Optical image of an NTS impenetrated into a HeLa cell [15]. ( g ) Fluorescence image of the NTS after adding 2 μM ionomycin into the extracellular medium [15]. ( h ) Real-time conductance measurement using an NTS after the injection of ionomycin [15].…”

Section: Sensingmentioning

confidence: 99%

“…As an example, an olfactory receptor-immobilized biosensor can selectively detect a target odorant at <10 fM concentration without responding to non-targeted odorants [12]. Furthermore, a CNT-based biosensor fabricated at the end of a nanoneedle can monitor the intracellular calcium concentration of living cells, which allows us to better understand the calcium signal pathways [15]. CNT-based biological sensors also have been applied for the detection of versatile biological substances such as antibiotics, environmental hormones, drugs and odorants [10,16–18].…”

Section: Introductionmentioning

confidence: 99%

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Nanoscale hybrid systems based on carbon nanotubes for biological sensing and control

et al. 2017

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This paper provides a concise review on the recent development of nanoscale hybrid systems based on carbon nanotubes (CNTs) for biological sensing and control. CNT-based hybrid systems have been intensively studied for versatile applications of biological interfaces such as sensing, cell therapy and tissue regeneration. Recent advances in nanobiotechnology not only enable the fabrication of highly sensitive biosensors at nanoscale but also allow the applications in the controls of cell growth and differentiation. This review describes the fabrication methods of such CNT-based hybrid systems and their applications in biosensing and cell controls.

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Section: Sensingmentioning

confidence: 99%

Section: Sensingmentioning

confidence: 99%

Section: Sensingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanoscale hybrid systems based on carbon nanotubes for biological sensing and control

et al. 2017

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“…(15) The progressive evolution of sensor fabrication technologies has moved the medical sensor field quite distinctly from the macroscale, through the microdomain and now into the nanoworld. (16) This enables the complexities of cellular and intracellular processes to be examined in detail at the fundamental level. In fact, there have also been significant benefits to research in sensor science and technology from the inspiration that derives from natural biological sensory systems, (17) for example, from the senses of sight, hearing, taste, smell and touch.…”

Section: Introductionmentioning

confidence: 99%

Micro- and Nanosensors for Medical and Biological Measurement

Rolfe¹

2012

Sensors and Materials

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Micro-and nanosensors have evolved rapidly in the last few decades and they have expanding roles within biology and medicine, where measurement science and technology is of key importance. The targets for measurement include a huge number of simple and complex molecules, physical quantities such as pressure, force, displacement and flow, and electrical and magnetic phenomena arising from the heart, brain, muscles and nerves. Routine clinical care of patients currently benefits from the use of macro-and microscale sensors based on electrical, electrochemical, acoustic, piezoelectric and optical principles. Disposable electrodes for recording biopotentials, such as the electrocardiogram and electroencephalogram, are common, whereas invasive electrochemical and optical fibre sensors for pressure, blood gases and pH are useful in intensive care. Microscale immobilised enzyme glucose sensors are largely confined to the analysis of small blood samples, their invasive use still facing technical challenges. Sensors constructed to the nanoscale using quantum dots and carbon nanotubes are now rapidly emerging, being aimed at more complex biomolecules such as DNA. Nanoparticles in general and surface-enhanced Raman spectroscopy also play important roles in these developments. The impact of micro-and nanosensors on the fundamental understanding of major biomedical challenges and on clinical diagnosis and care are highlighted here.

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Nanoscale Methods for Longitudinal Extraction of Intracellular Contents

Quek,

Tay

2024

Advanced Materials

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Longitudinal analysis of intracellular contents including gene and protein expression is crucial for deciphering the fundamentally dynamic nature of cells. This offers invaluable insights into complex tissue composition and behavior, and drive progress in disease diagnosis, biomarker discovery and drug development. Traditional longitudinal analysis workflows, involving the destruction of cells at various timepoints, limit insights to singular moments and fail to account for cellular heterogeneity. Current non‐destructive approaches, like temporal modelling with single‐cell RNA‐sequencing and live‐cell fluorescence imaging, either rely on biological assumptions or possess risk of cellular perturbation. Recent advances in nanoscale technologies for non‐destructive intracellular content extraction offer a promising solution to these challenges. These novel methods work at the nanoscale to non‐destructively access cellular membranes and can be broadly classified into three mechanisms: tip‐facilitated aspiration, membrane‐based and probe‐based methods. This perspective focuses on these emerging nanotechnologies for repeated intracellular content extraction. We discuss their potential in longitudinal analysis, address the critical requirements for effective repeated sampling, and explore the suitability of each technique for various applications. Furthermore, we highlight unresolved challenges in repeated sampling to encourage further research in this growing field.This article is protected by copyright. All rights reserved

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Nanoneedle Transistor-Based Sensors for the Selective Detection of Intracellular Calcium Ions

Cited by 53 publications

References 50 publications

Nanoscale hybrid systems based on carbon nanotubes for biological sensing and control

Nanoscale hybrid systems based on carbon nanotubes for biological sensing and control

Micro- and Nanosensors for Medical and Biological Measurement

Nanoscale Methods for Longitudinal Extraction of Intracellular Contents

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