Nanometrology and super-resolution imaging with DNA

Graugnard, Elton; Hughes, Will; Jungmann, Ralf; Kostiainen, Mauri A.; Linko, Veikko

doi:10.1557/mrs.2017.274

Cited by 25 publications

(26 citation statements)

References 53 publications

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“…[H2] DNA origami Among nanostructured materials, DNA origami 61 gained a prominent position due to versatility and high programmability of the DNA assembly 62 . However, their small size and complexity makes DNA origami rather challenging to be characterized with nanoscale accuracy; for this reason, super-resolution imaging of DNA origami is gaining growing attention since the first reports of such materials 63 .…”

Section: [H1] Srm For Molecular Observations In Vitromentioning

confidence: 99%

Super-resolution microscopy as a powerful tool to study complex synthetic materials

et al. 2019

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Section: [H1] Srm For Molecular Observations In Vitromentioning

confidence: 99%

Super-resolution microscopy as a powerful tool to study complex synthetic materials

et al. 2019

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“…[2][3][4][5] In recenty ears, programmable DNA origami nanostructures (DONs) [6][7][8][9][10] have been considered promising candidates for the development of tailored and multifunctional drug-delivery vehicles. However, in many of those applicationsa nd most notably in drug delivery,t heir stability and function may be compromised by the biological media.…”

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confidence: 99%

“…[2][3][4][5] In recenty ears, programmable DNA origami nanostructures (DONs) [6][7][8][9][10] have been considered promising candidates for the development of tailored and multifunctional drug-delivery vehicles. [2][3][4][5] In recenty ears, programmable DNA origami nanostructures (DONs) [6][7][8][9][10] have been considered promising candidates for the development of tailored and multifunctional drug-delivery vehicles.…”

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Real‐Time Observation of Superstructure‐Dependent DNA Origami Digestion by DNase I Using High‐Speed Atomic Force Microscopy

et al. 2019

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GuidoG rundmeier, [a] AdrianK eller,* [a] andV eikkoL inko* [a, b, c] DNA nanostructures have emerged as intriguing tools for numerous biomedical applications. However, in many of those applicationsa nd most notably in drug delivery,t heir stability and function may be compromised by the biological media. A particularly important issue for medicala pplications is their interaction with proteins such as endonucleases, which may degrade the well-defined nanoscale shapes.H erein, fundamental insights into this interaction are provided by monitoring DNase Id igestion of four structurally distinct DNA origami nanostructures (DONs) in real time and at as ingle-structure level by using high-speed atomic force microscopy.T he effect of the solid-liquidi nterface on DON digestioni sa lso assessed by comparison with experiments in bulk solution. It is shown that DON digestion is strongly dependentoni ts superstructure and flexibility and on the local topology of the individual structure.The rapidly evolvingf ield of DNA nanotechnology enables custom fabrication of various nanoscale shapes with unprecedented addressability; [1] these have found some fascinating implementations in materials science and especiallyi nm any biochemicala nd biophysical systems. [2][3][4][5] In recenty ears, programmable DNA origami nanostructures (DONs) [6][7][8][9][10] have been considered promising candidates for the development of tailored and multifunctional drug-delivery vehicles. [11][12][13][14] For therapeutic in vivo applications, the DON vehicles should preferably be compatible with the immunes ystem, resistant to nucleases, have as ufficiently long circulation half-life, and be able to maintain their shape at the ionic strengths of the biological fluids. [15] However,c oncerns have been raised regarding the stabilityo fD ONs and their performance in biological media. [16, 17] Although it has been shown that DONs can survive under low-cation conditions, [18,19] stability studies performedi n serum or cell culture media have yielded somewhat controversial and quite distinct results. [8,15,[19][20][21] Therefore, significant efforts have been madei nto coating ands tabilizing DONs under biologically relevant conditions. [17,[22][23][24][25] Herein, we study DON digestion by endonucleases (DNase I) in dependence of DON superstructure. DNase Ii sw idely present in serum and varioust issues,w hich makes it one of the most relevantt hreatst ot he stability of DONs in vivo. Previously,D ON cleavage by endonucleases was studied by employing ratherl ong timescales. [8,16] Therefore, thesea pproaches can only resolve the time points at which the whole or most of the nanostructure has been digested. Moreover,t hese experiments could neither reveals patial variations of DNase Is usceptibility within as ingle DON, nor facilitate parallel comparison of different structures under the same conditions. Herein, we thus employ high-speed atomicf orce microscopy (HS-AFM) [26] to study the degradation of four well-established and structurally disti...

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“…Concluding the issue, Graugnard et al 86 explore how dynamic and structural DNA nanotechnology can be used cooperatively to enable both new types of super-resolution imaging (DNA-PAINT), and calibration of super-resolution microscopes. Notably, the use of DNA origami rulers as calibration standards is the first commercial application of DNA nanostructures.…”

Section: In This Issuementioning

confidence: 99%