Nanometric Resolution in the Hydrodynamic Size Analysis of Ligand-Stabilized Gold Nanorods

Mehtala, Jonathan G.; Wei, Alexander

doi:10.1021/la502955h

Cited by 24 publications

(32 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The size analysis outcomes are similar and validate the accuracy of the latter method, in accord with other studies showing similar matches between TEM and NTA [35,39]. …”

Section: Resultssupporting

confidence: 89%

Eco-friendly (green) synthesis of magnetically active gold nanoclusters

Kadasala

Lin

Gilpin

et al. 2017

Science and Technology of Advanced Materials

Self Cite

View full text Add to dashboard Cite

Au–FexOy composite nanoparticles (NPs) are of great technological interest due to their combined optical and magnetic properties. However, typical syntheses are neither simple nor ecologically friendly, creating a challenging situation for process scale-up. Here we describe conditions for preparing Au–FexOy NPs in aqueous solutions and at ambient temperatures, without resorting to solvents or amphiphilic surfactants with poor sustainability profiles. These magnetic gold nanoclusters (MGNCs) are prepared in practical yields with average sizes slightly below 100 nm, and surface plasmon resonances that extend to near-infrared wavelengths, and sufficient magnetic moment (up to 6 emu g–1) to permit collection within minutes by handheld magnets. The MGNCs also produce significant photoluminescence when excited at 488 nm. Energy dispersive X-ray (EDX) analysis indicates a relatively even distribution of Fe within the MGNCs, as opposed to a central magnetic core.

show abstract

“…The size analysis outcomes are similar and validate the accuracy of the latter method, in accord with other studies showing similar matches between TEM and NTA [35,39]. …”

Section: Resultssupporting

confidence: 89%

Eco-friendly (green) synthesis of magnetically active gold nanoclusters

Kadasala

Lin

Gilpin

et al. 2017

Science and Technology of Advanced Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…The citrate-stabilized GNRs are compatible with surface conjugation chemistries commonly applied to metal colloids, and provide a reliable platform for making rational changes in chemical and biological properties as a function of surface modification. 19 …”

Section: Discussionmentioning

confidence: 99%

“…The cit-GNRs are fully dispersible in low-salt solutions, and are easily functionalized by further ligand exchange (discussed in a companion article). 19 …”

Section: Introductionmentioning

confidence: 99%

Citrate-Stabilized Gold Nanorods

et al. 2014

Self Cite

View full text Add to dashboard Cite

Stable aqueous dispersions of citrate-stabilized gold nanorods (cit-GNRs) have been prepared in scalable fashion by surfactant exchange from cetyltrimethylammonium bromide (CTAB)-stabilized GNRs, using polystyrenesulfonate (PSS) as a detergent. The surfactant exchange process was monitored by infrared spectroscopy, surface-enhanced Raman scattering (SERS), and X-ray photoelectron spectroscopy (XPS). The latter established the quantitative displacement of CTAB (by PSS) and of PSS (by citrate). The Cit-GNRs are indefinitely stable at low ionic strength, and are conducive to further ligand exchange without loss of dispersion stability. The reliability of the surface exchange process supports the systematic analysis of ligand structure on the hydrodynamic size of GNRs, as described in a companion paper.

show abstract

“…The high siRNA density is even more remarkable when taking into account that the loading was performed with co-adsorption of HS-PEG-folate, which is considered to adopt a mushroom-like conformation upon surface attachment. 49 DTC ligands are generally more aggressive than thiols, and the disordered conformation of individual PEG chains is poorly matched with the dense packing of oriented siRNA duplexes. We postulate that the chemisorption of thiolated PEG is limited to the GNR termini, where the radius of curvature is small and the adsorption of bulky ligands is facile.…”

Section: Preparation Of Gold Nanorod-sirna Carriersmentioning

confidence: 99%

“…44,45,46 This practice is less straightforward for GNRs due to their structural anisotropy and the challenges posed by cetyltrimethylammonium bromide (CTAB), a cationic surfactant used in GNR synthesis 22 whose efficient removal requires considerable attention to detail. 47,48,49 Several methods for exchanging CTAB with thiolated DNA have been described, 50,51,52 but have not been applied toward the loading of siRNA duplexes on GNRs.…”

Section: Introductionmentioning

confidence: 99%

siRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods

et al. 2018

Self Cite

View full text Add to dashboard Cite

We present a robust method for loading small interfering RNA (siRNA) duplexes onto the surfaces of gold nanorods (GNRs) at high density, using near-infrared (NIR) laser irradiation to trigger their intracellular release with subsequent knockdown activity. Citrate-stabilized GNRs were first coated with oleylsulfobetaine (OSB), a zwitterionic amphiphile with low cytotoxicity, which produced stable dispersions at high ionic strength. Amine-modified siRNA duplexes were converted into dithiocarbamate (DTC) ligands and adsorbed onto GNR surfaces in a single incubation step at 0.5 M NaCl, simplifying the charge screening process. The DTC anchors were effective at minimizing premature siRNA desorption and release, a common but often overlooked problem in the use of gold nanoparticles as oligonucleotide carriers. The activity of GNR-siRNA complexes was evaluated systematically against an eGFP-producing ovarian cancer cell line (SKOV-3) using folate receptor-mediated uptake. Efficient knockdown was achieved by using a femtosecond-pulsed laser source to release DTC-anchored siRNA, with essentially no contributions from spontaneous (dark) RNA desorption. GNRs coated with thiol-anchored siRNA duplexes were less effective, and also permitted low levels of knockdown activity without photothermal activation. Optimized siRNA delivery conditions were applied toward the targeted *

show abstract

Nanometric Resolution in the Hydrodynamic Size Analysis of Ligand-Stabilized Gold Nanorods

Cited by 24 publications

References 45 publications

Eco-friendly (green) synthesis of magnetically active gold nanoclusters

Eco-friendly (green) synthesis of magnetically active gold nanoclusters

Citrate-Stabilized Gold Nanorods

siRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods

Contact Info

Product

Resources

About