Nanomechanics of magnetically driven cellular endocytosis

Zablotskii, V.; Lunov, Oleg; Dejneka, A.; Jastrabı́k, L.; Polyakova, T.; Syrovets, Tatiana; Simmet, Th.

doi:10.1063/1.3656020

Cited by 45 publications

(22 citation statements)

References 22 publications

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“…Depending on cell type, exposure to a low or moderate static magnetic field may either increase or decrease Ca 2+ influx; for a review, see [8]. The possibility of monitoring and remotely controlling cellular endocytosis and/or exocytosis rates of superparamagnetic iron oxide (SPIO) nanoparticles using a magnetic field was recently demonstrated [9], [10]. A study of the direct influence of a magnetic field on a cell and the possibilities of magnetically controlling cellular motion, trapping and patterning, without the use of SPIO nanoparticles inserted in, or attached to the cells, is especially important because this approach avoids problems related to nanoparticle toxicity and removal.…”

Section: Introductionmentioning

confidence: 99%

Life on Magnets: Stem Cell Networking on Micro-Magnet Arrays

et al. 2013

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Interactions between a micro-magnet array and living cells may guide the establishment of cell networks due to the cellular response to a magnetic field. To manipulate mesenchymal stem cells free of magnetic nanoparticles by a high magnetic field gradient, we used high quality micro-patterned NdFeB films around which the stray field’s value and direction drastically change across the cell body. Such micro-magnet arrays coated with parylene produce high magnetic field gradients that affect the cells in two main ways: i) causing cell migration and adherence to a covered magnetic surface and ii) elongating the cells in the directions parallel to the edges of the micro-magnet. To explain these effects, three putative mechanisms that incorporate both physical and biological factors influencing the cells are suggested. It is shown that the static high magnetic field gradient generated by the micro-magnet arrays are capable of assisting cell migration to those areas with the strongest magnetic field gradient, thereby allowing the build up of tunable interconnected stem cell networks, which is an elegant route for tissue engineering and regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Life on Magnets: Stem Cell Networking on Micro-Magnet Arrays

et al. 2013

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“…The main idea of the optimization method relies on the fact that magnetic cell targeting can be improved by using a magnet of special shape that produces spatially modulated stray fields. 31,33 Optimizing magnet geometry for focusing magnetically labeled cells…”

Section: Magnetic Targeting In a Spinal Cord Lesionmentioning

confidence: 99%

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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Abstract:The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells' accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.

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“…We previously described endocytosis and cell labelling with these particles [25,26]. Additionally, we showed the feasibility of using static and PMFs to enhance endocytosis of such nanoparticles by different cell types [22,27]. Overall, the selected SPIONs represent well-characterized magnetic nanoparticles that show a robust response to magnetic fields.…”

Section: Characterization Of the Pulsed Magnetic Field (Pmf) System Amentioning

confidence: 91%

“…Briefly, the physicochemical characteristics of the SPIONs are summarized in Figure S1c. A detailed, full characterization of the SPIONs was reported elsewhere [23,24,[26][27][28][29][30]. We previously described endocytosis and cell labelling with these particles [25,26].…”

Section: Characterization Of the Pulsed Magnetic Field (Pmf) System Amentioning

confidence: 99%

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Lunov

Uzhytchak

Smolková

et al. 2019

Cancers

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Lysosome-activated apoptosis represents an alternative method of overcoming tumor resistance compared to traditional forms of treatment. Pulsed magnetic fields open a new avenue for controlled and targeted initiation of lysosomal permeabilization in cancer cells via mechanical actuation of magnetic nanomaterials. In this study we used a noninvasive tool; namely, a benchtop pulsed magnetic system, which enabled remote activation of apoptosis in liver cancer cells. The magnetic system we designed represents a platform that can be used in a wide range of biomedical applications. We show that liver cancer cells can be loaded with superparamagnetic iron oxide nanoparticles (SPIONs). SPIONs retained in lysosomal compartments can be effectively actuated with a high intensity (up to 8 T), short pulse width (~15 µs), pulsed magnetic field (PMF), resulting in lysosomal membrane permeabilization (LMP) in cancer cells. We revealed that SPION-loaded lysosomes undergo LMP by assessing an increase in the cytosolic activity of the lysosomal cathepsin B. The extent of cell death induced by LMP correlated with the accumulation of reactive oxygen species in cells. LMP was achieved for estimated forces of 700 pN and higher. Furthermore, we validated our approach on a three-dimensional cellular culture model to be able to mimic in vivo conditions. Overall, our results show that PMF treatment of SPION-loaded lysosomes can be utilized as a noninvasive tool to remotely induce apoptosis.

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Nanomechanics of magnetically driven cellular endocytosis

Cited by 45 publications

References 22 publications

Life on Magnets: Stem Cell Networking on Micro-Magnet Arrays

Life on Magnets: Stem Cell Networking on Micro-Magnet Arrays

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

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