Nanomechanics and ultrastructure of the internal mammary artery adventitia in patients with low and high pulse wave velocity

Chang, Zhuo; Paoletti, Paolo; Barrett, Steve; Chim, Ya Hua; Caamaño-Gutiérrez, Eva; Hansen, Morten Hartvig; Beck, Hans Christian; Rasmussen, Lars Melholt; Akhtar, Riaz

doi:10.1016/j.actbio.2018.04.036

Cited by 9 publications

(19 citation statements)

References 40 publications

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“…78 The accumulation of MFs in the adventitia produces collagen, fibronectin, tenascin, and elastin, which contributes to changes in vascular tone as well as the structure of the blood vessel. 70,79 Chang and colleagues 80 compared the nanomechanics and ultrastructure of the internal mammary artery adventitia in patients with low and high arterial stiffening, measured as pulse wave velocity. The internal mammary artery adventitia of patients with a high degree of arterial stiffness had significantly more collagen fibers with smaller fibril diameters compared with patients with a low degree of arterial stiffening.…”

Section: Cell and Ecm Changes In The Adventitia In Diseasementioning

confidence: 99%

Recent Developments in Vascular Adventitial Pathobiology

Tinajero

Gotlieb

2020

The American Journal of Pathology

100

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The adventitia, the outer layer of the blood vessel wall, may be the most complex layer of the wall and may be the master regulator of wall physiology and pathobiology. This review proposes a major shift in thinking to apply a functional lens to the adventitia rather than only a structural lens. Human and experimental in vivo and in vitro studies show that the adventitia is a dynamic microenvironment in which adventitial and perivascular adipose tissue cells initiate and regulate important vascular functions in disease, especially intimal hyperplasia and atherosclerosis. Although well away from the bloodwall interface, where much pathology has been identified, the adventitia has a profound influence on the population of intimal and medial endothelial, macrophage, and smooth muscle cell function. Vascular injury and dysfunction of the perivascular adipose tissue promote expansion of the vasa vasorum, activation of fibroblasts, and differentiation of myofibroblasts. This regulates further biologic processes, including fibroblast and myofibroblast migration and proliferation, inflammation, immunity, stem cell activation and regulation, extracellular matrix remodeling, and angiogenesis. A debate exists as to whether the adventitia initiates disease or is just an important participant. We describe a mechanistic model of adventitial function that brings together current knowledge and guides the design of future investigations to test specific hypotheses on adventitial pathobiology.

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Section: Cell and Ecm Changes In The Adventitia In Diseasementioning

confidence: 99%

Recent Developments in Vascular Adventitial Pathobiology

Tinajero

Gotlieb

2020

The American Journal of Pathology

100

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“…Based on the reference and normal values for carotid-femoral PWV [ 17 ], the 17 patients were split into two groups: low PWV (8.5 ± 0.7 ms −1 , n = 8 patients) and high PWV (13.4 ± 3.0 ms −1 , n = 9 patients). Full patient clinical parameters are presented in [ 1 ]. It should be noted that the tissue samples and patient cohorts are identical to those in our previous paper which focussed on the IMA adventitia [ 1 ].…”

Section: Methodsmentioning

confidence: 99%

“…PWV measurements are made in vivo by recording the transit time of blood across two points in the vascular system, typically from the carotid to the femoral artery. PWV is considered a powerful predictor of risk of morbidity and mortality in a general population [ 1 , 2 ]. However, arteries are associated with complex structural and biomechanical processes that occur as arteries stiffen with age and disease.…”

Section: Introductionmentioning

confidence: 99%

“…It is readily accessible as the repair artery during coronary artery bypass graft (CABG) operations. Although it is not involved in the carotid-femoral PWV pathway, we have recently shown using AFM that alterations in nanomechanical properties and collagen fibril morphology in the IMA adventitia are strongly correlated with high PWV [ 1 ]. Furthermore, the IMA has been used in a parallel study to identify small leucine-rich proteoglycans (SLRPs) which are associated with arterial stiffening [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although the PeakForce QNM has been recently used to probe biological samples under physiological conditions [ 10 – 14 ], our previous study was the first to apply PeakForce QNM to vascular tissues [ 1 ]. This paper builds on that study [ 1 ] which demonstrated with PeakForce QNM AFM [ 15 , 16 ] that the adventitia of the IMA is altered in patients with high PWV, in terms of both the nanoscale elastic modulus and the collagen fibril morphology. We now extend the approach and apply PeakForce QNM to the medial layer of the IMA in both dehydrated and hydrated conditions.…”

Section: Introductionmentioning

confidence: 99%

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AFM Characterization of the Internal Mammary Artery as a Novel Target for Arterial Stiffening

et al. 2018

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Using the atomic force microscopy- (AFM-) PeakForce quantitative nanomechanical mapping (QNM) technique, we have previously shown that the adventitia of the human internal mammary artery (IMA), tested under dehydrated conditions, is altered in patients with a high degree of arterial stiffening. In this study, we explored the nanoscale elastic modulus of the tunica media of the IMA in hydrated and dehydrated conditions from the patients with low and high arterial stiffening, as assessed in vivo by carotid-femoral pulse wave velocity (PWV). In both hydrated and dehydrated conditions, the medial layer was significantly stiffer in the high PWV group. The elastic modulus of the hydrated and dehydrated tunica media was significantly correlated with PWV. In the hydrated condition, the expression activity of certain small leucine-rich repeat proteoglycans (SLRPs), which are associated with arterial stiffening, were found to be negatively correlated to the medial elastic modulus. We also compared the data with our previous work on the IMA adventitia. We found that the hydrated media and dehydrated adventitia are both suitable for reflecting the development of arterial stiffening and SLRP expression. This comprehensive study of the nanomechanical properties integrated with the proteomic analysis in the IMAs demonstrates the possibility of linking structural properties and function in small biological samples with novel AFM methods. The IMA is a suitable target for predicting arterial stiffening.

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