Nanomechanical motion of <i>Escherichia coli</i> adhered to a surface

Lissandrello, Charles; İnci, Fatih; Francom, Matthew; Paul, Mark; Demirci, Utkan; Ekinci, K. L.

doi:10.1063/1.4895132

Cited by 69 publications

(56 citation statements)

References 38 publications

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“…In this pioneering work, we have exploited this nanomotion detector to monitor the viability of bacteria in the presence of different antibiotics and to assess rapidly (in less than 30 min) a complete bacterial antibiogram (18). Recently, other groups have used our technique to confirm our findings and to study the fluctuations induced by bacteria on a cantilever (19).…”

mentioning

confidence: 59%

Detecting nanoscale vibrations as signature of life

Kasas

Ruggeri

Benadiba

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

121

112

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The existence of life in extreme conditions, in particular in extraterrestrial environments, is certainly one of the most intriguing scientific questions of our time. In this report, we demonstrate the use of an innovative nanoscale motion sensor in life-searching experiments in Earth-bound and interplanetary missions. This technique exploits the sensitivity of nanomechanical oscillators to transduce the small fluctuations that characterize living systems. The intensity of such movements is an indication of the viability of living specimens and conveys information related to their metabolic activity. Here, we show that the nanomotion detector can assess the viability of a vast range of biological specimens and that it could be the perfect complement to conventional chemical life-detection assays. Indeed, by combining chemical and dynamical measurements, we could achieve an unprecedented depth in the characterization of life in extreme and extraterrestrial environments.nanomechanical sensors | extraterrestrial life | nanoscale fluctuations | living specimens | nanomotion detector T he existence of life in extreme conditions, in particular in extraterrestrial environments, is certainly one of the most intriguing scientific questions of our time. Indeed, the work of many scientists and organizations is focused on the discovery and on the consequent study of extremophiles and extraterrestrial organisms. The direct research for these kinds of life forms is usually conducted by deploying robotic crafts. These man-made vessels contain a suite of scientific analytical instrumentation that is specifically conceived to trace life signatures contained in the geological record. For instance, the search for life in our solar system started in 1975 with the Viking program and continues today. Future missions are planned to explore the presence of life on satellites of the giant planets, such as Europa (Jupiter) or Titan and Enceladus (Saturn). The biological instrumentation that is included in these vessels is complex, but up to now, it is mainly devoted to the chemical detection of molecules involved in living metabolism, as we know it on Earth.In this report, we show how a technique, the nanomotion detector, can be used in new life-searching instrumentation in Earth-bound and interplanetary missions. The technique exploits the sensitivity of nanomechanical sensors to transduce the small movements that characterize living systems. The intensity of such movements is an indication of the viability of the specimens and conveys information related to their metabolic activity. Here, we demonstrate that this simple technique can assess the viability of a vast range of biological specimens and that it could be the perfect complement to conventional chemical assays. Moreover, due to the simplicity of its working principle, a device based on this technology has negligible weight and requires very low electrical power, compared with other life-detector systems.Nanomechanical oscillators are extremely sensitive devices that are commonl...

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mentioning

confidence: 59%

Detecting nanoscale vibrations as signature of life

Kasas

Ruggeri

Benadiba

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

121

112

View full text Add to dashboard Cite

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“…The new generation of sensing platforms promises expanding sensing capabilities down to a single virus level. Some of the advances in other diagnostic areas such as recombinant antibody coupled nanomaterials (Kierny et al, 2012), graphene based wireless sensor (Mannoor et al, 2012) microcantilever resonator (Lissandrello, 2014), surface plasmon resonance aptasensor (Chuang et al, 2014) can be adapted to develop diagnostic tools for HBV, these new generation platforms and nanomaterials provide significant benefits. However, minimizing the cost of whole detection process is still a challenging problem since current methodologies in HBV screening are labor intensive and require expensive instruments.…”

Section: Resultsmentioning

confidence: 99%

Recent advances in micro/nanotechnologies for global control of hepatitis B infection

Yıldız

İnci

Wang

et al. 2015

Biotechnology Advances

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The control of Hepatits B virus (HBV) infection is a challenging task, specifically in developing countries where there is limited access to diagnostics and antiviral treatment mainly due to high costs and insufficient healthcare infrastructure. Although current diagnostic technologies can reliably detect HBV, they are relatively laborious, impractical and expensive for resource-limited settings. Advances in micro/nanotechnology are pioneering the development of new generation methodologies in diagnosis and screening of HBV. Owing to combination of nanomaterials (metal/inorganic nanopaticles, carbon nanotubes, etc.) with microfabrication technologies, utilization of miniaturized sensors detecting HBV and other viruses from ultra-low volume of blood, serum and plasma is realized. The state-of-the-art microfluidic devices with integrated nanotechnologies potentially allow for HBV screening at low cost. This review aims to highlight recent advances in nanotechnology and microfabrication processes that are employed for developing point-of-care (POC) HBV assays.

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“…Further, diagnosis and differentiation of HPV genotypes at the early stage would allow early treatment, thus decreasing severity of infection and mortality. Recent advances in microfluidics and labchip systems including optical [48]- [50], electrical [84], mechanical [85], and microchip platforms [86]- [88] allow intact pathogens including bacteria and viruses to be captured, thus enabling invaluable opportunities for postgenomic and proteomic analysis.…”

Section: Diagnostic Gaps Between Present Technologies and Unmet mentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

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| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

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Nanomechanical motion of Escherichia coli adhered to a surface

Cited by 69 publications

References 38 publications

Detecting nanoscale vibrations as signature of life

Detecting nanoscale vibrations as signature of life

Recent advances in micro/nanotechnologies for global control of hepatitis B infection

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

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