Nanomaterial Lipid-Based Carrier for Non-Invasive Capsaicin Delivery; Manufacturing Scale-Up and Human Irritation Assessment

Anantaworasakul, Phunsuk; Anuchapreeda, Songyot; Yotsawimonwat, Songwut; Naksuriya, Ornchuma; Lekawanvijit, Suree; Tovanabutra, Napatra; Wattanasri, Wajee; Buranapreecha, Narinthorn; Ampasavate, Chadarat

doi:10.3390/molecules25235575

Cited by 11 publications

(6 citation statements)

References 49 publications

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“…Short-term stability studies were conducted on the optimized nanoemulgel by keeping the formulation at various storage conditions—low temperature (4 ± 2 °C), room temperature (25 ± 2 °C), and high temperature (40 ± 2 °C)—for a period of 4 weeks and observing the changes in organoleptic properties and pH [ 20 ]. To assess the effect of different temperature conditions on the viscosity of the optimized formulation, the heating–cooling method was adopted under accelerated conditions for seven cycles as reported in the literature [ 23 ]. The developed nanoemulgel (n = 3) and the control formulation (n = 3) were stored in refrigerator temperature (4 ± 2 °C), room temperature (25 ± 2 °C), and high-temperature (40 ± 2 °C) conditions.…”

Section: Methodsmentioning

confidence: 99%

Development and Optimization of Methylcellulose-Based Nanoemulgel Loaded with Nigella sativa Oil for Oral Health Management: Quadratic Model Approach

et al. 2022

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The present study aimed to develop a local dental nanoemulgel formulation of Nigella sativa oil (NSO) for the treatment of periodontal diseases. NSO purchased from a local market was characterized using a GC–MS technique. A nanoemulsion containing NSO was prepared and incorporated into a methylcellulose gel base to develop the nanoemulgel formulation. The developed formulation was optimized using a Box–Behnken statistical design (quadratic model) with 17 runs. The effects of independent factors, such as water, oil, and polymer concentrations, were studied on two dependent responses, pH and viscosity. The optimized formulation was further evaluated for droplet size, drug release, stability, and antimicrobial efficacy. The developed formulation had a pH of 7.37, viscosity of 2343 cp, and droplet size of 342 ± 36.6 nm. Sustained release of the drug from the gel for up to 8 h was observed, which followed Higuchi release kinetics with non-Fickian diffusion. The developed nanoemulgel formulation showed improved antimicrobial activity compared to the plain NSO. Given the increasing emergence of periodontal diseases and antimicrobial resistance, an effective formulation based on a natural antibacterial agent is warranted as a dental therapeutic agent.

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Section: Methodsmentioning

confidence: 99%

Development and Optimization of Methylcellulose-Based Nanoemulgel Loaded with Nigella sativa Oil for Oral Health Management: Quadratic Model Approach

et al. 2022

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“…Table 1 delineates various prevalent drug nanocarriers, encompassing polymeric NPs, lipid NPs (such as liposomes, ethosomes, SLNs, and nanostructured lipid carriers), nanoemulsions, dendrimers, micelles, and inorganic NPs tailored for transdermal delivery. [18][19][20] Acne, psoriasis, ichthyosis [21][22][23] Anti-inflammatory [24,25] Nanostructured lipid carriers Yes No 50-500 Anti-inflammatory (Immuno-suppressive) [26] Local analgesic, anti-inflammatory [27,28] Antimicrobial [29] Anticancer [30] Nanoemulsions Yes No 10-1000 Cosmetic use: antioxidant, sunscreens, lipid carriers [31][32][33] Nonsteroidal anti-inflammatory drug carriers [34,35] Anticancer [36,37] Antimicrobial [38] Liposomes No Yes 100-200 Antimicrobial treatment [39,40] Eczema, psoriasis [41,42] Anti-inflammatory [43,44] Skin cancer [45,46] Local anesthesia [47,48] Ethosomes No Yes 100-200 Skin pathology [43,49] Skin cancer [50,51] Skin infections [52,53] Anti-inflammatory and analgesic [54,55] Micelles In the category of NPs, polymeric nanocapsules and nanospheres represent promising tools to increase the cutaneous permeation of pharmaceuticals and bioactive agents. Nanocapsules, characteri...…”

Section: Nanocarriers For Skin Applicationsmentioning

confidence: 99%

Characterization Methods for Nanoparticle–Skin Interactions: An Overview

Dzyhovskyi,

Romani,

Pula

et al. 2024

Life

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Research progresses have led to the development of different kinds of nanoplatforms to deliver drugs through different biological membranes. Particularly, nanocarriers represent a precious means to treat skin pathologies, due to their capability to solubilize lipophilic and hydrophilic drugs, to control their release, and to promote their permeation through the stratum corneum barrier. A crucial point in the development of nano-delivery systems relies on their characterization, as well as in the assessment of their interaction with tissues, in order to predict their fate under in vivo administration. The size of nanoparticles, their shape, and the type of matrix can influence their biodistribution inside the skin strata and their cellular uptake. In this respect, an overview of some characterization methods employed to investigate nanoparticles intended for topical administration is presented here, namely dynamic light scattering, zeta potential, scanning and transmission electron microscopy, X-ray diffraction, atomic force microscopy, Fourier transform infrared and Raman spectroscopy. In addition, the main fluorescence methods employed to detect the in vitro nanoparticles interaction with skin cell lines, such as fluorescence-activated cell sorting or confocal imaging, are described, considering different examples of applications. Finally, recent studies on the techniques employed to determine the nanoparticle presence in the skin by ex vivo and in vivo models are reported.

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“…The particle size and zeta potential of KME were carried out using a Zetasizer Nano ZS series instrument (Malvern Instruments, Worcestershire, UK), following the procedure outlined by Anantaworasakul et al [19]. A dilution of KME at a ratio of 1:100 v/v in deionized water was prepared.…”

Section: Characterization Of Kme Particle Size and Zeta Potential Ana...mentioning

confidence: 99%

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

Naksuriya,

Nitthikan,

Supadej

et al. 2023

Trends Sci

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Loading ketoconazole in microemulsions can enhance the solubility and permeation of ketoconazole into the skin. The systematic approach as quality by design (QbD) can help for better product and process understanding. This study aimed to develop topical ketoconazole-loaded microemulsions (KME) based on the QbD approach. After risk assessment, the design of experiment was utilized to determine the optimal ratio of water, isopropyl myristate, and surfactant mixture (polysorbate 80 and ethanol), which were chosen as critical material attributes. The particle size and polydispersity index, selected as critical quality attributes, were evaluated as responses. Then, characterizations, in vitro permeation, cytotoxicity, and antifungal activity studies were tested. As result, the equations from the D-optimal mixture design model successfully predicted the composition of the optimized formulation and obtained the acceptable design space. The optimized KME was 16.00 % w/w of isopropyl myristate, 73.50 % w/w of surfactant mixture, and 8.50 % w/w of water resulted in a water-in-oil system. The particle size and polydispersity index of KME were 21.1 ± 1.6 nm and 0.329 ± 0.020, respectively. KME showed prolonged skin retention after 24 h and was non-toxic. KME exhibited a broader zone of inhibition and lower inhibition concentration compared to unloaded ketoconazole against dermatophytes, indicating that KME enhanced effectiveness in antifungal activity for skin infection. The results of the KME meeting the quality target indicated a well-designed and suitable microemulsion for topical usage. Therefore, employing QbD in identifying the process and formulation is a promising approach for future development of high-quality topical KME products. HIGHLIGHTS The topical ketoconazole-loaded microemulsions (KME) were developed based on quality by design approach for further industrial production The D-optimal mixture design model successfully predicted the design space of desired KME formulation KME showed prolonged skin retention after 24 h KME was non-toxic for a topical dosage form KME exhibited antifungal activity greater than unloaded ketoconazole GRAPHICAL ABSTRACT

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Nanomaterial Lipid-Based Carrier for Non-Invasive Capsaicin Delivery; Manufacturing Scale-Up and Human Irritation Assessment

Cited by 11 publications

References 49 publications

Development and Optimization of Methylcellulose-Based Nanoemulgel Loaded with Nigella sativa Oil for Oral Health Management: Quadratic Model Approach

Development and Optimization of Methylcellulose-Based Nanoemulgel Loaded with Nigella sativa Oil for Oral Health Management: Quadratic Model Approach

Characterization Methods for Nanoparticle–Skin Interactions: An Overview

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

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