Nanomaterial‐Based Treatments for Medical Device‐Associated Infections

Tran, Nhiem; Tran, Phong A.

doi:10.1002/cphc.201200091

Cited by 56 publications

(43 citation statements)

References 219 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most effective method to resolve this problem is to deliver antibacterial agents locally from the implant surface. 8,9 Local controlled drug delivery will maintain the optimal concentrations of drugs at the specific site over prolonged periods without high systemic levels. Titania nanotubes (TNTs) fabricated on Ti implants via electrochemical anodization have attracted increasing attention because of their good biocompatibility and chemical and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Lin¹,

Tan²,

Duan³

et al. 2014

IJN

View full text Add to dashboard Cite

Titania nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specific site, thereby providing a promising strategy to prevent implant-associated infections. In this study we have fabricated titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length via electrochemical anodization. These nanotubes were loaded with 2 mg of gentamicin using a lyophilization method and vacuum drying. A standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the anti-infective ability of the gentamicin-loaded nanotubes (NT-G). Flat titanium (FlatTi) and nanotubes with no drug loading (NT) were also investigated and compared. We found that NT-G could significantly inhibit bacterial adhesion and biofilm formation compared to FlatTi or NT, and the NT-G with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended drug release time of NT-G with larger diameters. The NT also exhibited greater antibacterial ability than the FlatTi, while nanotubes with 80 nm or 120 nm diameters had better effects. Furthermore, human marrow derived mesenchymal stem cells were used to evaluate the effect of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells. Our results showed that NT-G and NT, especially those with 80 nm diameters, significantly promoted cell attachment, proliferation, spreading, and osteogenic differentiation when compared to FlatTi, and there was no significant difference between NT-G and NT with the same diameter. Therefore, nanotube modification and gentamicin loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Lin¹,

Tan²,

Duan³

et al. 2014

IJN

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, this is the first report of a bactericidal effect induced solely by nanoparticles immobilized on a surface, as gold is not bactericidal per se. 35 We consider one important factor to be the smaller contact area between the rigid cell wall of the bacteria and the surface, as verified by FIB slice and view, resulting in fewer sites available for adhesion receptor-ligand connections on nanostructured surfaces. This may in turn lead to a different microenvironment that is formed in the confined volume under the bacterial cells and in between the nanoparticles, resulting in, for example, accumulation of waste products, change of pH, and limited flow of nutrients because of the formation of a stagnant layer at the interface.…”

mentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

View full text Add to dashboard Cite

The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

show abstract

“…The inherent antibacterial properties of some metals and metal oxides have been known for centuries, causing them to be utilized extensively as bactericidal substances in infection control [43,44]. Metal and metal oxide nanoparticles exhibit striking physicochemical and biological properties distinct from their bulk forms, such as photocatalysis, photothermal effects, and reactive oxygen species (ROS)-stimulating activity [45].…”

Section: Nanotechnologies In Antimicrobial Treatmentmentioning

confidence: 99%

“…Up to 54% of all catheters have been shown to be infected with bacteria and can cause many serious complications such as urinary tract infection, bloodstream infection, and even death [131]. Implant-associated infection occurs at low to moderate rates (7.4% of cardiovascular and 4.3% of orthopedic implants infected in the United States [132]) but is very problematic, since treatment is implant removal and replacement if standard antibiotic treatment is not successful [44]. The initiation of device-related infection usually involves bacterial adhesion to the device or the patient-derived glycoprotein coating (conditioning film), subsequent microbial proliferation, and the development of biofilms.…”

Section: Antimicrobial Nanotechnologies In Medical Devicesmentioning

confidence: 99%

Nanomedicine in the management of microbial infection – Overview and perspectives

et al. 2014

View full text Add to dashboard Cite

For more than 2 billion years, microbes have reigned on our planet, evolving or outlasting many obstacles they have encountered. In the 20th century, this trend took a dramatic turn with the introduction of antibiotics and vaccines. Nevertheless, since then, microbes have progressively eroded the effectiveness of previously successful antibiotics by developing resistance, and many infections have eluded conventional vaccine design approaches. Moreover, the emergence of resistant and more virulent strains of bacteria has outpaced the development of new antibiotics over the last few decades. These trends have had major economic and health impacts at all levels of the socioeconomic spectrum – we need breakthrough innovations that could effectively manage microbial infections and deliver solutions that stand the test of time. The application of nanotechnologies to medicine, or nanomedicine, which has already demonstrated its tremendous impact on the pharmaceutical and biotechnology industries, is rapidly becoming a major driving force behind ongoing changes in the antimicrobial field. Here we provide an overview on the current progress of nanomedicine in the management of microbial infection, including diagnosis, antimicrobial therapy, drug delivery, medical devices, and vaccines, as well as perspectives on the opportunities and challenges in antimicrobial nanomedicine.

show abstract

Nanomaterial‐Based Treatments for Medical Device‐Associated Infections

Cited by 56 publications

References 219 publications

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Nanomedicine in the management of microbial infection – Overview and perspectives

Contact Info

Product

Resources

About