Nanomaterial-based optical and electrochemical techniques for detection of methicillin-resistant Staphylococcus aureus: a review

Gill, Atal A.S.; Singh, Sima; Thapliyal, Neeta; Karpoormath, Rajshekhar

doi:10.1007/s00604-018-3186-7

Cited by 56 publications

(26 citation statements)

References 135 publications

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“…Early rapid diagnosis of S. aureus is valuable for the timely use of effective antimicrobial therapy to improve prognosis. Previous studies have reported many methods for the rapid detection of S. aureus, such as PCR, protein markers, and immunofluorescence, but each method has advantages and disadvantages, as mentioned above, and has not been widely used in clinical practice (Wu et al, 2013;Hirvonen, 2014;Gill et al, 2019). Biosensors are a multidisciplinary technology that integrates biology with other disciplines, such as physics and chemistry.…”

Section: Discussionmentioning

confidence: 99%

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Cui

Zhou

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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Staphylococcus aureus (S. aureus) is one of the most common clinical pathogenic bacteria with strong pathogenicity and usually leads to various suppurative infections with high fatality. Traditional bacterial culture for the detection of S. aureus is prone to diagnosis and antimicrobial treatment delays because of its long-time consumption and low sensitivity. In this study, we successfully developed a quantum dots immunofluorescence biosensor for S. aureus detection. The biosensor combined the advantages of biosensors with the high specificity of antigen-antibody immune interactions and the high sensitivity and stability of quantum dots fluorescence. The results demonstrated that the biosensor possessed high specificity and high sensitivity for S. aureus detection. The detection limit of S. aureus reached 1 × 104 CFU/ml or even 1 × 103 CFU/ml, and moreover, the fluorescence intensity had a significant positive linear correlation relationship with the logarithm of the S. aureus concentration in the range of 103–107 CFU/ml (correlation coefficient R2 = 0.9731, P = 0.011). A specificity experiment showed that this biosensor could effectively distinguish S. aureus (1 × 104 CFU/ml and above) from other common pathogenic (non-S. aureus) bacteria in nosocomial infections, such as Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli. Additionally, the whole detection procedure spent only 2 h. In addition, the biosensor in this study may not be affected by the interference of the biofilm or other secretions since the clinical biological specimens are need to be fully liquefied to digest and dissolve viscous secretions such as biofilms before the detection procedure of the biosensor in this study. In conclusion, the biosensor could meet the need for rapid and accurate S. aureus detection for clinical application.

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Section: Discussionmentioning

confidence: 99%

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Cui

Zhou

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 91%

Recent progress and challenges on the bioassay of pathogenic bacteria

2020

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The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

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“…If the analyte (e.g., mecA gene) binds to the bioreceptor, a transducer converts this interaction into a signal that can be displayed and quantified. Biosensors are usually nanomaterial-based [ 133 ]. Biosensors can be phenotypic (if they detect phenotypical features such as growth in the presence of antibiotics or cell wall components) or genotypic (if they detect genes) [ 134 ].…”

Section: Emerging Methods For Antimicrobial Resistance Detection In S Aureusmentioning

confidence: 99%

“…In recent years, biosensor-based systems showed promising results for the rapid, portable, easy and low-cost detection of pathogens, making them good candidates for POC diagnostics. The challenges of developing biosensors are as follows: a DNA amplification step is often required to enhance the signal intensity and detect low concentrations of analytes (bacteria or genes) in clinical samples; and currently, multiplexing approaches in biosensors are less common compared to PCR assays [ 81 , 92 , 133 ]. In 2019, Gill and colleagues reviewed the principal optical and electrochemical sensor technologies based on nanomaterials to detect MRSA [ 133 ].…”

Section: Emerging Methods For Antimicrobial Resistance Detection In S Aureusmentioning

confidence: 99%

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

Sanchini¹

2022

Diagnostics

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Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of infections in humans, such as skin and soft tissue infections, pneumonia, food poisoning or sepsis. Historically, S. aureus was able to rapidly adapt to anti-staphylococcal antibiotics and become resistant to several classes of antibiotics. Today, methicillin-resistant S. aureus (MRSA) is a multidrug-resistant pathogen and is one of the most common bacteria responsible for hospital-acquired infections and outbreaks, in community settings as well. The rapid and accurate diagnosis of antimicrobial resistance in S. aureus is crucial to the early initiation of directed antibiotic therapy and to improve clinical outcomes for patients. In this narrative review, I provide an overview of recent phenotypic and molecular diagnostic methods for antimicrobial resistance detection in S. aureus, with a particular focus on MRSA detection. I consider methods for resistance detection in both clinical samples and isolated S. aureus cultures, along with a brief discussion of the advantages and the challenges of implementing such methods in routine diagnostics.

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Nanomaterial-based optical and electrochemical techniques for detection of methicillin-resistant Staphylococcus aureus: a review

Cited by 56 publications

References 135 publications

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Recent progress and challenges on the bioassay of pathogenic bacteria

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

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