Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip

Maruyama, Hisataka; Kotani, Kyosuke; Masuda, Taisuke; Honda, Ayae; Takahata, Tatsuro; Arai, Fumihito

doi:10.1007/s10404-010-0739-4

Cited by 60 publications

(42 citation statements)

References 18 publications

(17 reference statements)

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“…PCC 6803 as single cell measurement This method can be used to measure the physiological properties in a single cell and can be used for analysis of cell to cell communication. For example, analysis of influenza virus activity inside the infected cell will be realized by measuring the physiological conditions before and after virus infection [21]. This technique will make a great contribution in cell biology.…”

Section: Discussionmentioning

confidence: 99%

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

Maruyama

Kariya

Nakamura

et al. 2012

2012 IEEE/RSJ International Conference on Intelligent Robots and Systems

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In this paper, we proposed long-lifetime and high-sensitive measurement using fluorescent intensity by compensation of photo-bleaching with the difference compensation for cell measurement. Photo-bleaching of fluorescence is one of the most crucial factors to cause measurement error in physiological measurement. In our approach, difference of fluorescent intensity between imaging time is compensated using single exponential function to make the difference amount uniform in each imaging time. We evaluated the dependencies of measurable time and the sensitivity by fluorescent lifetime and the decay coefficient of fluorescent lifetime using numerical simulation. From these results, the proposed method has much higher sensitivity and longer measurable time against conventional compensation method of photo-bleaching. By using the proposed method, we demonstrate the photosynthesis activity of single Synechocystis sp. PCC 6803 in the micro chamber with gas barrier layer.

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Section: Discussionmentioning

confidence: 99%

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

Maruyama

Kariya

Nakamura

et al. 2012

2012 IEEE/RSJ International Conference on Intelligent Robots and Systems

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“…For example, analysis of influenza virus activity inside the infected cell will be realized by measuring the physiological conditions before and after virus infection [26]. This technique will make a great contribution in cell biology.…”

Section: Discussionmentioning

confidence: 99%

Temperature measurement by color analysis of fluorescent spectrum using cell investigation tool impregnated with quantum dot for cell measurement on a microfluidic chip

Maruyama

Tomita

Masuda

et al. 2011

2011 IEEE/RSJ International Conference on Intelligent Robots and Systems

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In this paper, we proposed cell investigation tool for measurement of physiological conditions of single cell. We developed a novel temperature measurement method using temperature dependence of the quantum dot. The cell investigation tool encapsulates the quantum dot and adheres to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We proposed image processing of the tools using the color information for temperature measurement. We calibrated the temperature using the tool impregnated with quantum dot by several color spaces such as HSV and YCrCb (H: hue, S: saturation, V: values (brightness). Y: brightness, Cr: color difference of red, Cb: color difference of blue). Y and Cr represent the monotone decrease according to the temperature increase, while H and Cb do not represent monotone variation. Measurement value by Cr is stable rather than the values by Y and V information because the color difference is robust against brightness fluctuation. From these results, we confirmed temperature measurement using Cr information suitable for cell analysis. Sensitivity is -1.3 %/K and accuracy is 0.3 K.

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“…Studies have demonstrated that it is possible to trap and accumulate an ensemble of virus particles from a static solution using microDEP [10][11][12], but capture and manipulation of individual virus particles, particularly those from a highflow-rate solution (as needed for the rapid concentration of dilute virus samples) have not been developed. A few studies have demonstrated that single virus particles in a flow can be trapped between the gap of two nanoelectrodes [13] or using optical tweezers coupled with a microDEP concentrator [14]. However, these techniques are expensive and can only be used in low-throughput studies.…”

Section: Introductionmentioning

confidence: 99%

Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays

et al. 2013

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This work describes efficient manipulation of bacteriophage virus particles using a nanostructured dielectrophoresis (DEP) device. The non-uniform electric field for DEP is created by utilizing a nanoelectrode array (NEA) made of vertically aligned carbon nanofibers (VACNFs) versus a macroscopic indium tin oxide electrode in a "points-and-lid" configuration integrated in a microfluidic channel. The capture of the virus particles has been systematically investigated versus the flow velocity, sinusoidal AC frequency, peak-to-peak voltage, and virus concentration. The DEP capture at all conditions is reversible and the captured virus particles are released immediately when the voltage is turned off. At the low virus concentration (8.9×10 4 pfu·ml −1 ), the DEP capture efficiency up to 60% can be obtained. The virus particles are individually captured at isolated nanoelectrode tips and accumulate linearly with time. Due to the comparable size, it is more effective to capture virus particles than larger bacterial cells with such NEA based DEP devices. This technique can be potentially utilized as a fast sample preparation module in a microfluidic chip to capture, separate, and concentrate viruses and other biological particles in small volumes of dilute solutions in a portable detection system for field applications.

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Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip

Cited by 60 publications

References 18 publications

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

Ultra long-lifetime and high-sensitive fluorescent measurement using difference compensation method for single cell analysis

Temperature measurement by color analysis of fluorescent spectrum using cell investigation tool impregnated with quantum dot for cell measurement on a microfluidic chip

Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays

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