Nanoluciferase-based cell fusion assay for rapid and high-throughput assessment of SARS-CoV-2-neutralizing antibodies in patient samples

Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

“…This is hydrolyzed at a slow rate by cellular esterases and releases furimazine throughout the experiment. 28 , 29 Incubation with 1 nM TNFα resulted in a large stimulation of HiBiT-E-selectin cell surface expression that began after 2 h and peaked at 8 h ( Figure 7 A). The difference in the peak cell surface expression of HiBiT E-selectin between TNFα (1 nM) and vehicle-treated cells was substantial (p < 0.001; Figure 7 B).…”