Nanoliter atmospheric pressure photoionization-mass spectrometry for direct bioanalysis of polycyclic aromatic hydrocarbons

Tan, Siyuan; Yin, Xiaobo; Feng, Lulu; Wang, Juduo; Xue, Zhichao; You, Jane; Dai, Xinhua; Gong, Xiaoyun; Fang, Xiang

doi:10.1039/d3an00442b

Cited by 2 publications

(5 citation statements)

References 39 publications

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“…These new hybrid ion source devices can realize the high-sensitivity detection of trace compounds (especially low-polarity compounds) in cells or expand the types of detection. Our research team introduced a novel nanoliter atmospheric pressure photoionization (nano-APPI) source ( Figure 4 ) ( Tan et al, 2023 ). This innovation empowers the sensitive detection of PAHs in individual samples.…”

Section: Single-cell Ms Methodologies Based On Four Ionization Techni...mentioning

confidence: 99%

“… Schematic illustration of nano-APPI for low-polarity PAHs analysis. Adapted with permission from Tan et al (2023) . Analyst 148, 3,730–3,739 ( Tan et al, 2023 ).…”

Section: Single-cell Ms Methodologies Based On Four Ionization Techni...mentioning

confidence: 99%

Section: Single-cell Ms Methodologies Based On Four Ionization Techni...mentioning

confidence: 99%

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Recent developments in ionization techniques for single-cell mass spectrometry

Zeng,

Xia,

Yin

et al. 2023

Front. Chem.

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The variation among individual cells plays a significant role in many biological functions. Single-cell analysis is advantageous for gaining insight into intricate biochemical mechanisms rarely accessible when studying tissues as a whole. However, measurement on a unicellular scale is still challenging due to unicellular complex composition, minute substance quantities, and considerable differences in compound concentrations. Mass spectrometry has recently gained extensive attention in unicellular analytical fields due to its exceptional sensitivity, throughput, and compound identification abilities. At present, single-cell mass spectrometry primarily concentrates on the enhancement of ionization methods. The principal ionization approaches encompass nanoelectrospray ionization (nano-ESI), laser desorption ionization (LDI), secondary ion mass spectrometry (SIMS), and inductively coupled plasma (ICP). This article summarizes the most recent advancements in ionization techniques and explores their potential directions within the field of single-cell mass spectrometry.

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Section: Single-cell Ms Methodologies Based On Four Ionization Techni...mentioning

confidence: 99%

“… Schematic illustration of nano-APPI for low-polarity PAHs analysis. Adapted with permission from Tan et al (2023) . Analyst 148, 3,730–3,739 ( Tan et al, 2023 ).…”

Section: Single-cell Ms Methodologies Based On Four Ionization Techni...mentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in ionization techniques for single-cell mass spectrometry

Zeng,

Xia,

Yin

et al. 2023

Front. Chem.

Self Cite

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show abstract

“…In contrast, atmospheric pressure chemical ionization and photoionization (APCI, APPI) are suitable for analyzing nonpolar substances, but they have lower analytical capabilities for polar substances. 13 It is hard to realize simultaneous MS analysis of substances with different polarities by a single ionization source. Hybrid ionization sources coupled with MS analysis have been used for simultaneous detection of different polar substances in drugs, environmental samples, tumor tissue, and body fluids 14−18 ical ionization (nanoESI-APCI) for the detection of couples of compounds within single onion cells and human cells, respectively.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In recent years, electrospray ionization (ESI)-based single-cell MS techniques have been proven to be applicable for metabolite analysis of microalgae cells. , However, only polar small molecular substances in microalgae were detected; it is difficult to detect low-polarity but high-economic value biosynthesis products in single microalgae. In contrast, atmospheric pressure chemical ionization and photoionization (APCI, APPI) are suitable for analyzing nonpolar substances, but they have lower analytical capabilities for polar substances . It is hard to realize simultaneous MS analysis of substances with different polarities by a single ionization source.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Metabolite Analysis of Unicellular Microalgae by Orthogonal Hybrid Ionization Label-Free Mass Cytometry

Yao,

Yang,

Wang

et al. 2024

Anal. Chem.

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Microalgae metabolite analysis is fundamental for the rational design of metabolic engineering strategies for the biosynthesis of high-value products. Mass spectrometry (MS) has been utilized for single-cell microalgae analysis. However, limitations in the detection throughput and polarities of detectable substances make it difficult to realize high-throughput screening of high-performance microalgae. Herein, a plasma-assisted label-free mass cytometry, named as PACyESI-MS, was proposed combining the advantages of orthogonal hybrid ionization and high-throughput MS analysis, which realized rapid metabolite detection of single microalgae. The cell detection throughput of PACyESI-MS was up to 52 cells/min. Dozens of the critical primary and secondary metabolites within single microalgae were detected simultaneously, including pigments, lipids, and energy metabolites. Furthermore, metabolite changes of Chlamydomonas reinhardtii and Haematococcus pluvialis under nitrogen deficiency stress were studied. Discrimination of Chlamydomonas under different nutrient conditions was realized using single-cell metabolite profiles obtained by PACyESI-MS. The relationships between the accumulation of bioactive astaxanthin and changes in functional primary metabolites of Haematococcus were investigated. It was demonstrated that PACyESI-MS can detect the flexible change of metabolites in single microalgae cells under different nutritional conditions and during the synthesis of high-value products, which is expected to become an important tool for the design of metabolic engineering-based high-performance microalgae factories.

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Nanoliter atmospheric pressure photoionization-mass spectrometry for direct bioanalysis of polycyclic aromatic hydrocarbons

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are a class of low-polarity environmental contaminants that have severe carcinogenic effects and have drawn worldwide attention. However, there remains a challenge for current mass spectrometric...

Cited by 2 publications

References 39 publications

Recent developments in ionization techniques for single-cell mass spectrometry

Recent developments in ionization techniques for single-cell mass spectrometry

High-Throughput Metabolite Analysis of Unicellular Microalgae by Orthogonal Hybrid Ionization Label-Free Mass Cytometry

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