Nanoliposomal encapsulation of chia oil for sustained delivery of α‐linolenic acid

Abstract

Summary Chia oil is a popular source of ω‐3 fatty acids, typically α‐linolenic acid. This study reports the encapsulation of chia oil in nanoliposome to protect ω‐3 fatty acids and to obtain a sustained release of chia oil during digestion. Nanoliposomal encapsulation was carried out using solvent evaporation, followed by sonication. The encapsulation process was conducted using different lipid contents, with different concentrations of soy phosphatidylcholine (S), Tween 80 (T) and volumes of the aqueous phase. The maximum encapsulation efficiency was found to be 88.31%, and the average particle size was 49.25 nm; a moderate repulsion among the particles was observed. Differential scanning calorimetry study revealed enhanced thermal stability of chia oil in nanoliposomes. A negligible release (3.39%) of encapsulated chia oil was observed in the simulated gastric fluid, and a 74.72% sustained release was recorded in the simulated intestinal fluid. This formulation can be a suitable supplement of ω‐3 fatty acids for food and therapeutic applications.

Liposomal encapsulation of omega‐3 fatty acid and α‐lipoic acid conjugate for cow milk fortification

“…This indicates EE of 80.24 ± 0.49% and 76.38 ± 0.58% for CO and LA liposome, respectively. In our previous publication on nanoliposomes, the EE of CO nanoliposome was found to be 88.31 ± 0.64% (Choudhary et al., 2021). The decreased value in the current study could be owing to the competitive encapsulation of LA and CO in the nanoliposome.…”

“…The nanoliposome was prepared by thin film hydration method at the optimized conditions already published by us (Choudhary et al., 2021). The thin film of lipid obtained from soya phosphatidylcholine and Tween 80 (1:1) (containing 1 g CO) was hydrated using inclusion complex (in PBS), and nanoliposomes were formulated using a probe sonicator (PRO 650, Labman Scientific Instruments Private Limited, Chennai, India).…”

“…The thin film of lipid obtained from soya phosphatidylcholine and Tween 80 (1:1) (containing 1 g CO) was hydrated using inclusion complex (in PBS), and nanoliposomes were formulated using a probe sonicator (PRO 650, Labman Scientific Instruments Private Limited, Chennai, India). The nanoliposome thus obtained (LA‐HPβCD‐in CO) was dialyzed against PBS buffer (pH 7.0, 50 mM) for 24 hr to separate the encapsulated CO and inclusion complex from the unencapsulated one (Choudhary et al., 2021).…”

“…The ALA content of nanoliposome was estimated following the method detailed by Choudhary et al. (2021). Briefly, the fat from the nanoliposome was extracted and converted into fatty acid methyl ester (FAME) (ISO 5509:2000).…”

“…These lipid vesicles offer several advantages such as entrapment and controlled release of hydrophilic, lipophilic, and amphiphilic materials, the possibility of large‐scale production utilizing natural ingredients, as well as they render enhanced targetability (Dutta et al, 2018). In fact, we have already published the formulation of the CO liposome and its detailed characterization, including the sustained release of CO from its liposome (Choudhary et al., 2021). In accordance, with the present work, a combined liposomal encapsulation of both CO and LA has been investigated to obtain a conjugate of ALA and LA for the secured delivery of both antioxidants.…”