Nanog-mediated stem cell properties are critical for MBNL3-associated paclitaxel resistance of ovarian cancer

Sun, Xueqin; Diao, Xinghua; Zhu, Xiaolin; Yin, Xuexue; Cheng, Guangying

doi:10.1093/jb/mvab021

Cited by 8 publications

(4 citation statements)

References 36 publications

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“… 31 Previous studies demonstrated that MBNL3 affects radio sensitivity and paclitaxel resistance in carcinogenesis. 32 , 33 MBNL3 is overexpressed in HCC, although likewise PSAT1. However, MBNL3 is an HCC-specific oncofetal protein for the unique therapeutic target of HCC, which overexpressed in fetal liver tissues, silenced in adult liver and reproduced in HCC tissues.…”

Section: Discussionmentioning

confidence: 98%

TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition

Cao

Yang

Luo

et al. 2023

Hepatology Communications

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Background: HCC characterizes malignant metastasis with high incidence and recurrence. Thus, it is pivotal to discover the mechanisms of HCC metastasis. TATA-box-binding protein (TBP), a general transcriptional factor (TF), couples with activators and chromatin remodelers to sustain the transcriptional activity of target genes. Here, we investigate the key role of TBP in HCC metastasis. Methods: TBP expression was measured by PCR, western blot, and immunohistochemistry. RNA-sequencing was performed to identify downstream proteins. Functional assays of TBP and downstream targets were identified in HCC cell lines and xenograft models. Luciferase reporter and chromatin immunoprecipitation assays were used to demonstrate the mechanism mediated by TBP. Results: HCC patients showed high expression of TBP, which correlated with poor prognosis. Upregulation of TBP increased HCC metastasis in vivo and in vitro, and muscleblind-like-3 (MBNL3) was the effective factor of TBP, positively related to TBP expression. Mechanically, TBP transactivated and enhanced MBNL3 expression to stimulate exon inclusion of lncRNA-paxillin (PXN)-alternative splicing (AS1) and, thus, activated epithelial-mesenchymal transition for HCC progression through upregulation of PXN. Conclusions: Our data revealed that TBP upregulation is an HCC enhancer mechanism that increases PXN expression to drive epithelial-mesenchymal transition.

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Section: Discussionmentioning

confidence: 98%

TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition

Cao

Yang

Luo

et al. 2023

Hepatology Communications

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“…Some studies have proved that MBNL3 is involved in regulating the progression of some cancers through serving as a facilitator [ 6 - 9 ]. Nevertheless, the regulatory functions of MBNL3 in GAC remain vague.…”

Section: Discussionmentioning

confidence: 99%

“…Recent reports revealed that MBNL3 expression is dysregulated in diversified cancers, thereby affecting tumor growth and development. For instance, higher expression of MBNL3 is positively associated with PTX resistance and results in poor prognosis in ovarian cancer [ 6 ]. In addition, MBNL3 affects cell invasion in pancreatic ductal adenocarcinoma [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

MBNL3 Acts as a Target of miR-302e to Facilitate Cell Proliferation, Invasion and Angiogenesis of Gastric Adenocarcinoma via AKT/VEGFA Pathway

Tang,

Huang,

Jiang

et al. 2024

J. Microbiol. Biotechnol.

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Gastric adenocarcinoma (GAC) is a common, malignant type of tumor in human, and is accompanied with higher mortality. Muscleblind-like 3 (MBNL3) was found to be a pivotal participator in aggravating this cancer’s progression. However, the regulatory effects of MBNL3 on GAC development have not been investigated. We therefore sought to study the functions of MBNL3 in GAC progression. In this study, it was demonstrated that MBNL3 exhibited higher expression, and GAC patients with higher MBNL3 expression had poor prognosis. Overexpression of MBNL3 facilitated, and knockdown of MBNL3 suppressed cell proliferation, invasion, and angiogenesis in GAC. Further experiments showed that miR-302e targets MBNL3. Rescue assays then uncovered that the miR-302e/MBNL3 axis aggravated GAC progression. In addition, MBNL3 activated the AKT/VEGFA pathway, and the suppressive regulatory impacts of MBNL3 knockdown on GAC cell proliferation, invasion, and angiogenesis could be rescued after 740 Y-P treatment. Through in vivo assay, it was proved that MBNL3 accelerated tumor growth in vivo. In conclusion, MBNL3 acted as a target of miR-302e to facilitate cell proliferation, invasion, and angiogenesis of gastric adenocarcinoma through the AKT/VEGFA pathway. Our findings illustrate that MBNL3 may be an available bio-target for GAC treatment.

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“…Activation of CASP3 transcription has been found to suppress the proliferation of cancer cells and to promote cell apoptosis in multiple cancers ( Li et al, 2016 ; Luo et al, 2018 ; Zhu et al, 2019 ). MBNL3 participates in paclitaxel resistance in ovarian cancer ( Sun et al, 2021 ). SEPT2 is required for the progression of human breast cancer via MEK/ERK activation ( Zhang et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Ribosomal protein L8 regulates the expression and splicing pattern of genes associated with cancer-related pathways

XU,

YANG,

SONG

et al. 2023

Turkish Journal of Biology

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Background/aim Ribosomal proteins have been shown to perform unique extraribosomal functions in cell apoptosis and other biological processes. Ribosomal protein L8 (RPL8) not only has important nonribosomal regulatory functions but also participates in the oncogenesis and development of tumors. However, the specific biological functions and pathways involved in this process are still unknown. Materials and methods RPL8 was overexpressed (RPL8-OE) in HeLa cells. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Transcriptome sequencing was performed to analyze the differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs) by RPL8-OE, both of which were validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. Results RPL8-OE inhibited cell proliferation and promoted cell apoptosis. RPL8 regulated the differential expression of many oncogenic genes and the occurrence of RASEs. Many DEGs and RASE genes (RASGs) were enriched in tumorigenesis and tumor progression-related pathways, including angiogenesis, inflammation, and regulation of cell proliferation. RPL8 could regulate the RASGs enriched in the negative regulation of apoptosis, consistent with its proapoptosis function. Furthermore, RPL8 may influence cancer-related DEGs by modulating the alternative splicing of transcription factors. Conclusion RPL8 might affect the phenotypes of cancer cells by altering the transcriptome profiles, including gene expression and splicing, which provides novel insights into the biological functions of RPL8 in tumor development.

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Nanog-mediated stem cell properties are critical for MBNL3-associated paclitaxel resistance of ovarian cancer

Cited by 8 publications

References 36 publications

TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition

TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition

MBNL3 Acts as a Target of miR-302e to Facilitate Cell Proliferation, Invasion and Angiogenesis of Gastric Adenocarcinoma via AKT/VEGFA Pathway

Ribosomal protein L8 regulates the expression and splicing pattern of genes associated with cancer-related pathways

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