NANOG Decoy Oligodeoxynucleotide–Encapsulated Niosomes Nanocarriers: A Promising Approach to Suppress the Metastatic Properties of U87 Human Glioblastoma Multiforme Cells

Abstract

Recently, advances in the synthesis and development of multifunctional nanoparticle platforms have opened up great opportunities and advantages for specifically targeted delivery of genes of interest. BSA-coated niosome structures (NISM@B) can potentially improve the efficiency in vitro delivery of nucleic acid molecules and the transfection of genes. Few studies have reported the combined use of niosomes with nucleic acid as therapeutic agents or decoy oligodeoxynucleotides (ODNs). Herein, we synthesized NISM@B to encapsulate NANOG decoy ODN (NISM@B-DEC), after which the physicochemical characteristics and in vitro and in vivo properties of NISM@B-DEC were investigated. Our results regarding physicochemical characteristics revealed that the stable niosome nanocarrier system was successfully synthesized with a regular spherical shape and narrow size distribution with proper zeta-potential values and had an appropriate biocompatibility. The ODN release from the niosome nanocarrier system exhibited controlled and pH-dependent behavior as the best models to explain the ODN release profile. NISM@B-DEC was efficiently taken up by human glioblastoma cells (U87) and significantly inhibited cell growth. Finally, blockage of the NANOG pathway by NISM@B-DEC resulted in G1 cell cycle arrest, apoptosis, and cell death. In addition, NISM@B-DEC caused a significant decrease in tumor formation and improved wound-healing efficiency of the U87 cells. These findings confirm that NISM@B-DEC could potentially suppress the metastatic ability of these cells. It can be concluded that the presented nanocarrier system can be a promising approach for targeted gene delivery in cancer therapy.

Hybrid Cell Membrane-Functionalized Biomimetic Nanoparticles for Targeted Therapy of Osteosarcoma

“… 24 In addition, bovine serum albumin (BSA)-coated niosomes could also effectively encapsulate genetic drugs such as decoy oligodeoxynucleotides to inhibit the metastasis of glioblastoma multiforme, which could be a promising approach for targeted gene delivery in cancer therapy. 25 The PLGA nanoparticles coated with hybrid membrane from the osteosarcoma cell membrane and macrophage cell membrane also show high biocompatibility and high targeting ability to osteosarcoma cells. In this study, we chose the human osteosarcoma cell line 143B and mice monocyte-macrophage cell line RAW264.7.…”

In silico design of quadruplex aptamers against the spike protein of SARS-CoV-2

“…Previous reports have indicated that in silico methods such as molecular docking studies could be useful for studying the interactions between oligonucleotides and protein molecules which were proved effective for therapeutic application studies [ 28 , 29 ]. In the current study, a new approach has been developed to design of G-quadruplex DNA aptamer against SARS-CoV-2 spike protein.…”

Mitigated Oxidative Stress and Cognitive Impairments in Transient Global Ischemia using Niosomal Selegiline-NBP delivery

“…Subsequently, 5 mL of the obtained solution was added to the flask and mixed by vortex for 1 min. The mixture was then emulsified by 30 min of sonication in a water bath at 50°C [ 17 ].…”