Nanog and transcriptional networks in embryonic stem cell pluripotency

Pan, Guoshun; Thomson, James A.

doi:10.1038/sj.cr.7310125

Cited by 532 publications

(415 citation statements)

References 44 publications

(69 reference statements)

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“…It indicated that porcine Nanog had similar properties in PFF, at least partially, which were described for Nanog in primate and mouse ESCs. In both humans and mice, research shows that Nanog could bind to the upstream element of Oct4 and cooperate with other core transcription factors to activate Oct4 expression thereby maintaining the pluripotency (Boyer et al, 2005;Rodda et al, 2005;Pan and Thomson, 2007). In mESCs, Nanog interacts with Sall4 in a way similar with that of Oct4 and Sox2 to activate each other, thus helps to set up the pluripotency regulatory circuits .…”

Section: Discussion Transient Elevation Of Nanog Expression Can Activmentioning

confidence: 99%

“…We speculate that transient high Nanog expression can activate part of the pluripotency related genes in PFF, but it is not enough for inducing PFF to a fully pluripotent state, thus cannot set up the stable regulatory circuit. Pan et al also indicate that the key pluripotent factors always work together to control the whole set of pluripotency machinery rather than work individually (Pan and Thomson, 2007). So in a partially transformed PFF, these activated pluripotent genes can be easily silenced in many ways including DNA highly methylating.…”

Section: Pluripotent Genes Expression Cannot Be Maintained During G41mentioning

confidence: 99%

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Overexpression Nanog Activates Pluripotent Genes in Porcine Fetal Fibroblasts and Nuclear Transfer Embryos

Zhang

Luo

Bou

et al. 2011

The Anatomical Record

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Nanog as an important transcription factor plays a pivotal role in maintaining pluripotency and in reprogramming the epigenome of somatic cells. Its ability to function on committed somatic cells and embryos has been well defined in mouse and human, but rarely in pig. To better understand Nanog's function on reprogramming in porcine fetal fibroblast (PFF) and nuclear transfer (NT) embryo, we cloned porcine Nanog CDS and constructed pcDNA3.1 (þ)/Nanog and pEGFP-C1/Nanog overexpression vectors and transfected them into PFFs. We studied the cell biological changes and the expression of Nanog, Oct4, Sox2, Klf4, C-myc, and Sall4 in transfected PFFs. We also detected the development potential of the cloned embryos harboring Nanog stably overexpressed fibroblasts and the expression of Oct4, Sox2, and both endogenous and exogenous Nanog in these embryos. The results showed that transient overexpression Nanog in PFF could activate the expression of Oct4 (5-fold), C-myc (2-fold), and Sall4 (5-fold) in somatic cells, but they could not be maintained during G418 selection. In NT embryos, although Nanog overexpression did not have a significant effect on blastocyst development rate and blastocyst cell number, it could significantly activate the expression of endogenous Nanog, Oct4, Sox2 to 160-fold, 93-fold, and 182-fold, respectively (P < 0.05). Our results demonstrate that Nanog could interact with and activate other pluripotent genes both in PFFs and embryos. Anat Rec, 294:1809Rec, 294: -1817Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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Section: Discussion Transient Elevation Of Nanog Expression Can Activmentioning

confidence: 99%

Section: Pluripotent Genes Expression Cannot Be Maintained During G41mentioning

confidence: 99%

Overexpression Nanog Activates Pluripotent Genes in Porcine Fetal Fibroblasts and Nuclear Transfer Embryos

Zhang

Luo

Bou

et al. 2011

The Anatomical Record

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“…Two characteristic properties of hESCs, pluripotency, a capacity to differentiate into all fetal and adult cell lineages, and the ability to undergo symmetrical self-renewing divisions, are largely controlled at the transcriptional level (reviewed in Chen and Daley, 2008). In undifferentiated hESCs, pluripotency genes such as OCT4 (also called POU5F1 ), NANOG and SOX2 are expressed, whereas genes involved in differentiation are transcriptionally inactive (reviewed in Sun et al, 2006; Pan and Thomson, 2007). Decreased expression of pluripotency genes induces differentiation (Niwa et al, 2000), and thus proper transcriptional regulation is essential for self-renewal of undifferentiated hESCs.…”

Section: Introductionmentioning

confidence: 99%

Non-canonical TAF complexes regulate active promoters in human embryonic stem cells

Maston

Zhu

Chamberlain

et al. 2012

eLife

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The general transcription factor TFIID comprises the TATA-box-binding protein (TBP) and approximately 14 TBP-associated factors (TAFs). Here we find, unexpectedly, that undifferentiated human embryonic stem cells (hESCs) contain only six TAFs (TAFs 2, 3, 5, 6, 7 and 11), whereas following differentiation all TAFs are expressed. Directed and global chromatin immunoprecipitation analyses reveal an unprecedented promoter occupancy pattern: most active genes are bound by only TAFs 3 and 5 along with TBP, whereas the remaining active genes are bound by TBP and all six hESC TAFs. Consistent with these results, hESCs contain a previously undescribed complex comprising TAFs 2, 6, 7, 11 and TBP. Altering the composition of hESC TAFs, either by depleting TAFs that are present or ectopically expressing TAFs that are absent, results in misregulated expression of pluripotency genes and induction of differentiation. Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.DOI: http://dx.doi.org/10.7554/eLife.00068.001

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“…Undifferentiated human cells express at high level the pluripotency markers OCT4, SOX2 and NANOG, the cell surface markers stage-specific antigen-4 (SSEA4) and TRA-1-60; and alkaline phosphatase [169][170][171]. The levels of expression of these markers decline rapidly once differentiation has begun.…”

Section: Markers For Differentiation Into Cardiomyocyte Lineagementioning

confidence: 99%

We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells

Arabadjiev¹,

Petkova²,

Chakarov³

et al. 2014

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Citation: Arabadjiev A, Petkova R, Chakarov S, Pankov R, Zhelev N. We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells. AbstractHuman cell lines, including disease cell lines are often superior to routine animal models for the purposes of rapid and safe assessment of the effects of different agents that may modulate myocardial functioning under physiological and pathological conditions. There are several currently existing methodologies for derivation of cardiomyocyte-like cells by targeted differentiation from pluripotent cells and by reprogramming/ transdifferentiation from other types of cells (multipotent progenitors, somatic cells, etc). The present paper reviews the potential sources of cells capable of differentiation along the cardiomyocyte lineage; the existing methodologies for targeted differentiation, outlining the specificities of each method; and the markers for differentiation along the mesodermal and the cardiogenic lineage. The yield of robustly beating cells expressing cardio-specific proteins derived by any of the existing methods, however, still rarely exceeds 70-90 %, even with the newly developed approaches for increasing the differentiation capacity. There still is significant variance in the results obtained by different research groups and even between different experiments carried out in the same laboratory, with the same type of cells and same general type of protocol. Derivation of new lines of human pluripotent and multipotent stem cells according to standardised protocols and in completely defined; xeno-free conditions may increase the reliability and reproducibility of research and speed up the development of potential clinical applications.

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Nanog and transcriptional networks in embryonic stem cell pluripotency

Cited by 532 publications

References 44 publications

Overexpression Nanog Activates Pluripotent Genes in Porcine Fetal Fibroblasts and Nuclear Transfer Embryos

Overexpression Nanog Activates Pluripotent Genes in Porcine Fetal Fibroblasts and Nuclear Transfer Embryos

Non-canonical TAF complexes regulate active promoters in human embryonic stem cells

We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells

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