nanog 5′-upstream sequence, DNA methylation, and expression in gametes and early embryo reveal striking differences between teleosts and mammals

Marandel, Lucie; Labbé, Catherine; Bobe, Julien; Bail, Pierre‐Yves Le

doi:10.1016/j.gene.2011.10.037

Cited by 28 publications

(30 citation statements)

References 40 publications

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“…Samples were homogenised in Trizol reagent (Invitrogen) with Precellys®24 (Bertin Technologies, Montigny-le-Bretonneux, France) according to the process described previously 15 . Luciferase control RNA (Promega), 10 pg per 1.9 mg of embryo/alevin was added to each sample to allow for data normalisation as previously described 57, 58 . Total RNA was then extracted according to the Trizol manufacturer’s instructions and 1 µg of total RNA was subsequently reverse-transcribed to cDNA using The Super-Script III RNAse H-Reverse transcriptase kit (Invitrogen) with random primers (Promega, Charbonniéres, France).…”

Section: Methodsmentioning

confidence: 99%

“…Each qPCR assay included replicate samples (duplicate of reverse transcription and PCR amplification) and negative controls (reverse transcriptase- and cDNA template-free samples). Data were subsequently normalised to the exogenous luciferase transcript abundance in samples diluted at 1:25 using the E method (Light Cycler software) as previously described 58 .…”

Section: Methodsmentioning

confidence: 99%

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Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout

Liu

Plagnes-Juan

Geurden

et al. 2017

Sci Rep

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Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L−1 O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L−1 O2 hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L−1 O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout

Liu

Plagnes-Juan

Geurden

et al. 2017

Sci Rep

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“…As discussed below, even though these mechanisms are set in a different context in mammalian development, an understanding of the ancestral mechanism will give us deeper insight into this process in mammals (Cañon et al 2011; Sánchez-Sánchez et al 2011; Marandel et al 2012; Lee et al 2013). …”

Section: Phase 2—from Egg To Embryomentioning

confidence: 99%

Growing an Embryo from a Single Cell: A Hurdle in Animal Life: Figure 1.

O’Farrell

2015

Cold Spring Harb Perspect Biol

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A requirement that an animal be able to feed to grow constrains how a cell can grow into an animal, and it forces an alternation between growth (increase in mass) and proliferation (increase in cell number). A growth-only phase that transforms a stem cell of ordinary proportions into a huge cell, the oocyte, requires dramatic adaptations to help a nucleus direct a 105-fold expansion of cytoplasmic volume. Proliferation without growth transforms the huge egg into an embryo while still accommodating an impotent nucleus overwhelmed by the voluminous cytoplasm. This growth program characterizes animals that deposit their eggs externally, but it is changed in mammals and in endoparasites. In these organisms, development in a nutritive environment releases the growth constraint, but growth of cells before gastrulation requires a new program to sustain pluripotency during this growth.

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“…The primer sets used for ck49, ck8, nanog, pou2, sox2, c-myca1, c-myca2 cDNA detection were previously validated by Mauger et al [32] and Marandel et al [27][28][29]. The primer sequences, concentrations and annealing temperatures are summarized in Table 1.…”

Section: Reverse Transcriptase Quantitative Pcr (Rt-qpcr) Assay Of Thmentioning

confidence: 99%

“…nn Significant difference (po0.05) between the two explant systems at a given time of culture. E X P E R I M E N T A L C E L L R E S E A R C H 3 3 5 ( 2 0 1 5 ) 2 3 -3 8 the possible candidates, the c-myca1, c-myca2, sox2, nanog and pou2 were chosen because nucleotide sequence of the corresponding cDNA was established in goldfish, and their expression was demonstrated during the embryonic development [27][28][29]. Contrarily to the differentiation marker genes described above, all the transcription factors were expressed at undetectable (nanog and pou2) or very low (c-myca1, c-myca2, sox2) levels in the two culture systems.…”

Section: Temporal and Spatial Cellular Distribution Of The Marker Genmentioning

confidence: 99%

Stabilization of gene expression and cell morphology after explant recycling during fin explant culture in goldfish

Chênais

Lareyre

Bail

et al. 2015

Experimental Cell Research

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nanog 5′-upstream sequence, DNA methylation, and expression in gametes and early embryo reveal striking differences between teleosts and mammals

Cited by 28 publications

References 40 publications

Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout

Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout

Growing an Embryo from a Single Cell: A Hurdle in Animal Life: Figure 1.

Stabilization of gene expression and cell morphology after explant recycling during fin explant culture in goldfish

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