NanoFlares for the detection, isolation, and culture of live tumor cells from human blood

Halo, Tiffany L.; McMahon, Kaylin M.; Angeloni, Nicholas L.; Xu, Yilin; Wang, Wei; Chinen, Alyssa B.; Malin, Dmitry; Strekalova, Elena; Cryns, Vincent L.; Cheng, Chonghui; Mirkin, Chad A.; Thaxton, C. Shad

doi:10.1073/pnas.1418637111

Cited by 197 publications

(150 citation statements)

References 27 publications

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“…Previously our group introduced the concept of the Nanoflare, a spherical nucleic acid (SNA) conjugate capable of quantifying RNA concentration in live cells with single cell resolution (17)(18)(19)(20)(21)(22). The Nanoflare consists of a 13-nm gold nanoparticle core functionalized with a densely packed, highly oriented shell of oligonucleotides designed to be antisense to a target RNA transcript.…”

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confidence: 99%

Quantification and real-time tracking of RNA in live cells using Sticky-flares

Briley

Bondy

Randeria

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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103

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We report a novel spherical nucleic acid (SNA) gold nanoparticle conjugate, termed the Sticky-flare, which enables facile quantification of RNA expression in live cells and spatiotemporal analysis of RNA transport and localization. The Sticky-flare is capable of entering live cells without the need for transfection agents and recognizing target RNA transcripts in a sequence-specific manner. On recognition, the Sticky-flare transfers a fluorophore-conjugated reporter to the transcript, resulting in a turning on of fluorescence in a quantifiable manner and the fluorescent labeling of targeted transcripts. The latter allows the RNA to be tracked via fluorescence microscopy as it is transported throughout the cell. We use this novel nanoconjugate to analyze the expression level and spatial distribution of β-actin mRNA in HeLa cells and to observe the real-time transport of β-actin mRNA in mouse embryonic fibroblasts. Furthermore, we investigate the application of Stickyflares for tracking transcripts that undergo more extensive compartmentalization by fluorophore-labeling U1 small nuclear RNA and observing its distribution in the nucleus of live cells.he study of RNA is a critical component of biological research and in the diagnosis and treatment of disease. Recently, the localization of mRNA has been identified as an essential process for a number of cellular functions, including restricting the production of certain proteins to specific compartments within cells (1). For instance, synaptic potentiation, the basis of learning and memory, relies on the local translation of specific mRNAs in pre-and postsynaptic compartments (2). Likewise, the misregulation of RNA distribution is associated with many disorders, including mental retardation, autism, and cancer metastasis (3-5). However, despite the significant role of mRNA transport and localization in cellular function, the available methods to visualize these phenomena are severely limited. For example, FISH, the most commonly used technique to analyze spatial distribution of RNA, requires fixation and permeabilization of cells before analysis (6). As a result, analysis of dynamic RNA distribution is restricted to a single snapshot in time (7,8). With such a limitation, understanding the translocation of RNA with respect to time, cell cycle, or external stimulus is difficult if not impossible. Furthermore, fixed cell analysis is a lengthy and highly specialized procedure due to the number of steps necessary to prepare a sample. Fixation, permeabilization, blocking, and staining processes each require optimization and vary based on cell type and treatment conditions, rendering FISH prohibitively complicated in many cases. Likewise, live cell analysis platforms such as molecular beacons require toxic transfection techniques, such as microinjection or lipid transfection, and are rapidly sequestered to the nucleus on cellular entry (9, 10). Recently more sophisticated live cell analyses have been developed that use genetic engineering to introduce exogenous hybrid gene...

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confidence: 99%

Quantification and real-time tracking of RNA in live cells using Sticky-flares

Briley

Bondy

Randeria

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

103

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“…These Au nanoparticles have been extensively studied and used as therapeutic agents (drug delivery) [46], diagnosis agents [47], photothermaltherapy [48] and imaging agents [49]. Their tiny size, which meets the dimension of the most biological compounds, high surface area, relatively ease preparation, ease surface functionization make them particularly fascinating towards medical application.…”

Section: Nanoscale Gold Structuresmentioning

confidence: 99%

Nanoscale Metal Particles as Nanocarriers in Targeted Drug Delivery System

Lah¹,

Samykano²,

Trigueros³

2016

JNMR

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In the recent years, noble metal particles such as gold (Au) and silver (Ag) have been used progressively as efficient and safe nanoscale drug carriers in treating malignant as cites of cancerous cells. These single crystal structures of functionalised therapeutic particles with the size less than 100 nm in diameter had proven offered an excellent function to modulate the oxidative stress and toxicity at affected membrane cells particularly to achieve the site-specific delivery of drugs. This mini-review will highlight the current advances of Au and Ag nanoscale particles as smart chemotherapeutic molecule carriers to these malignancies in impeding cancer cell activities locally. The paper also reviewed valuable insights of their efficacy in maintaining and precisely control the drugs release level within the therapeutic windows.

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“…For in vitro biological applications, synthetic methods for surface-loading high-density coverages of DNA have enabled efficient cell delivery for various in-cell, live-cell assays (Fig. 3) (75). Other surface chemistries have enabled a myriad of electrochemical, chemical, and optical platforms for sensing and in vitro diagnostics (76)(77)(78).…”

Section: Inorganic Nanoparticles and Related Nanomaterials In Biomedimentioning

confidence: 99%

Nanotechnologies for biomedical science and translational medicine

Heath

2015

Proc. Natl. Acad. Sci. U.S.A.

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In 2000 the United States launched the National Nanotechnology Initiative and, along with it, a well-defined set of goals for nanomedicine. This Perspective looks back at the progress made toward those goals, within the context of the changing landscape in biomedicine that has occurred over the past 15 years, and considers advances that are likely to occur during the next decade. In particular, nanotechnologies for health-related genomics and single-cell biology, inorganic and organic nanoparticles for biomedicine, and wearable nanotechnologies for wellness monitoring are briefly covered.nanotechnology | biotechnology | nanomedicine

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NanoFlares for the detection, isolation, and culture of live tumor cells from human blood

Cited by 197 publications

References 27 publications

Quantification and real-time tracking of RNA in live cells using Sticky-flares

Quantification and real-time tracking of RNA in live cells using Sticky-flares

Nanoscale Metal Particles as Nanocarriers in Targeted Drug Delivery System

Nanotechnologies for biomedical science and translational medicine

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