Nanofiltration membranes for challenging environment

Han, Runlin; Zhang, Shouhai; Sui, Yan

doi:10.1016/b978-0-12-821506-7.00004-1

Cited by 1 publication

(1 citation statement)

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“…GNP (Figure S1A) shows a high crystallinity, with a tight and sharp peak at 2θ of 26.5°, attributed to the (002) reflection plane with a d‐spacing of 3.36. [ 16 ] The peak confirms presence of stacked layers of graphene in GNP. A smaller peak is observed at 54.6°, assigned to the (004) plane of bulk graphite, possibly due to impurities.…”

Section: Resultsmentioning

confidence: 94%

Immobilization of Cyclodextrin glycosyltransferase onto three dimensional‐ hydrophobic and two dimensional‐ hydrophilic supports: A comparative study

Ogunbadejo,

Aljahoushi,

Alzamly

et al. 2023

Biotechnology Journal

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Cyclodextrin glycosyltransferase (CGTase) degrades starch into cyclodextrin via enzymatic activity. In this study, we immobilize CGTase from Thermoanaerobacter sp. on two supports, namely graphene nanoplatelets (GNP) consisting of short stacks of graphene nanoparticles and a calcium‐based two‐dimensional metal organic framework (Ca‐TMA). The uptakes of CGTase on GNP and Ca‐TMA reached 40 and 21 mg g−1 respectively, but immobilized CGTase on Ca‐TMA showed a higher specific activity (38 U mg−1) than that on GNP (28 U mg−1). Analysis of secondary structures of CGTase, shows that immobilization reduces the proportion of β‐sheets in CGTase from 56% in the free to 49% and 51.3% for GNP and Ca‐TMA respectively, α‐helix from 38.5% to 18.1 and 37.5%, but led to increased β‐turns from 5.5 to 40% and 11.2% for GNP and Ca‐TMA, respectively. Lower levels of conformational changes were observed over the more hydrophilic Ca‐TMA compared to hydrophobic GNP, resulting in its better activity. Increased β‐turns were found to correlate with lower β‐CD production, while more β‐sheets and α‐helix favored more β‐CD. Reusability studies revealed that GNP retains up to 74% of initial CGTase activity, while Ca‐TMA dropped to 33% after eight consecutive uses. The results obtained in this work provide insight on the effect of support's surface properties on CGTase performance and can assist in developing robust CGTase‐based biocatalysts for industrial application.

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Section: Resultsmentioning

confidence: 94%