Nanofibrous vascular scaffold prepared from miscible polymer blend with heparin/stromal cell-derived factor-1 alpha for enhancing anticoagulation and endothelialization

Wang, Weizhong; Liu, Dinghua; Li, Dejian; Du, Haibo; Zhang, Jingtian; You, Zhengwei; Li, Maoquan; He, Chuanglong

doi:10.1016/j.colsurfb.2019.06.065

Cited by 29 publications

(25 citation statements)

References 42 publications

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“…Among various bioactive molecules, heparin has gained considerable attention since it is an effective and widely used anticoagulant in clinics [13,19,20]. The use of heparin can not only minimize the formation of thrombus on artificial surfaces, but also inhibit the growth of smooth muscle cells [21]. However, there is no report in the literature regarding the dual promotions of incorporation of nano-scaled fibers into electrospun grafts and surface immobilization of heparin on dual fiber surfaces.…”

Section: Introductionmentioning

confidence: 99%

Heparinization and hybridization of electrospun tubular graft for improved endothelialization and anticoagulation

Zhang

Zhu

et al. 2021

Materials Science and Engineering: C

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Constructing biomimetic structure and immobilizing antithrombus factors are two effective methods to ensure rapid endothelialization and long-term anticoagulation for small-diameter vascular grafts. However, few literatures are available regarding simultaneous implementation of these two strategies. Herein, a nano-micro-fibrous biomimetic graft with a heparin coating was prepared via a step-by-step in situ biosynthesis method to improve potential endothelialization and anticoagulation. The 4-mm-diameter tubular graft consists of electrospun cellulose acetate (CA) microfibers and entangled bacterial nanocellulose (BNC) nanofibers with heparin coating on dual fibers. The hybridized and heparinized graft possesses suitable pore structure that facilitates endothelia cells adhesion and proliferation but prevents infiltration of fibrous tissue and blood leakage. In addition, it shows higher mechanical properties than those of bare CA and hybridized CA/BNC grafts, which match well with native blood vessels. Moreover, this dually modified graft exhibits improved blood compatibility and endothelialization over the counterparts without hybridization or heparinization according to the testing results of platelet adhesion, cell morphology, and protein expression of von Willebrand Factor. This novel graft with dual modifications shows promising as a new small-diameter vascular graft. This study provides a guidance for promoting endothelialization and blood compatibility by dual modifications of biomimetic structure and immobilized bioactive molecules.

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Section: Introductionmentioning

confidence: 99%

Heparinization and hybridization of electrospun tubular graft for improved endothelialization and anticoagulation

Zhang

Zhu

et al. 2021

Materials Science and Engineering: C

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“…However, the use of autografts is limited by the shortage of donors and secondary site injury. 1,2 Therefore, artificial grafts have become the focus of research in the field of vascular replacement. Although large vascular grafts are successfully used in the clinic, smalldiameter vascular grafts (o6 mm) remain a huge challenge due to intimal hyperplasia and thrombosis.…”

Section: Introductionmentioning

confidence: 99%

Controllable synthesis of biomimetic nano/submicro-fibrous tubes for potential small-diameter vascular grafts

et al. 2020

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“…Among the suggested approaches is the formation of a thromboresistant inner surface to prevent implant thrombosis in the early postoperative period [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

A Technology for Anti-Thrombogenic Drug Coating of Small-Diameter Biodegradable Vascular Prostheses

et al. 2020

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The aim of the study was to develop a technology for anti-thrombogenic drug coating of biodegradable porous scaffolds and to evaluate the physicomechanical and hemocompatible properties of functionally active vascular prostheses with and without a drug coating. Materials and Methods. Vascular prostheses from polyhydroxybutyrate/valerate and polycaprolactone with the incorporated vascular endothelial growth factor, the main fibroblast growth factor, and the chemoattractant SDF-1α were made by emulsion electrospinning. Additional surface modification of the prostheses was carried out by forming a hydrogel coating of polyvinylpyrrolidone capable of binding drugs as a result of complexation. Unfractionated heparin and iloprost were used as anti-thrombogenic drugs. Results. We show that after the modification of vascular prostheses with heparin and iloprost, a 5.8-fold increase in the Young’s modulus value was noted, which indicated a greater stiffness of these grafts compared to the unmodified controls. Platelet aggregation on the surface of heparin + iloprost coated vascular prostheses was 3.3 times less than that with the unmodified controls, and 1.8 times less compared to intact platelet-rich plasma. The surface of vascular prostheses with heparin and iloprost was resistant to adhesion of platelets and blood proteins. Conclusion. Drug (unfractionated heparin and iloprost) coating of the surface of biodegradable prostheses significantly improved the anti-thrombogenic properties of these grafts but contributed to the increased stiffness of the prostheses.

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Nanofibrous vascular scaffold prepared from miscible polymer blend with heparin/stromal cell-derived factor-1 alpha for enhancing anticoagulation and endothelialization

Cited by 29 publications

References 42 publications

Heparinization and hybridization of electrospun tubular graft for improved endothelialization and anticoagulation

Heparinization and hybridization of electrospun tubular graft for improved endothelialization and anticoagulation

Controllable synthesis of biomimetic nano/submicro-fibrous tubes for potential small-diameter vascular grafts

A Technology for Anti-Thrombogenic Drug Coating of Small-Diameter Biodegradable Vascular Prostheses

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