Nanofibrous scaffolds with biomimetic structure

Abstract

This research studies the effect of using a grid-like pattern as a collector on increasing the pore size of the electrospun gelatin/cellulose acetate/elastin scaffolds. The morphological study showed an enlargement in pore size and a decline in fiber diameter in comparison with the scaffold fabricated using conventional flat sheet collectors. The use of the pattern increased the swelling ratio and degradation rate of the scaffold. Investigating the tensile properties of scaffolds revealed that the patterned collector increased the elongation at break up to 145%. In vitro experiments revealed the patterned scaffold as a good substrate for attachment and proliferation of fibroblast cells. Overall, our results indicated that the patterned scaffold of gelatin/cellulose acetate/elastin could provide a better microenvironment for fibroblast cells compared to the conventional scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 370-376, 2018.

Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

“…By contrast, the scaffolds with a higher content of cellulose acetate and a lower content of gelatin (ratio 75:25) appeared to be suitable for low-adherent wound dressings [208]. Other cellulose acetate-based scaffolds with potential for skin tissue engineering include composite 3D electrospun cellulose acetate/pullulan scaffolds, which promoted the adhesion and growth of mouse L929 fibroblasts [209], and composite biomimetic nanofibrous gelatin/cellulose acetate/elastin scaffolds, which promoted the adhesion and growth of human gingival fibroblasts [210]. CNCs have a very large elasticity modulus (about 130 GPa), which is similar to that of Kevlar, and they have high strength (about 7 GPa).…”

Wound dressings: Current advances and future directions

“…The largest organ in human is skin with an area of about 2 m 2 . The skin has three different layers: including epidermis, dermis, and hypodermis, which intrinsically are self‐renewable and have various functions .…”

Theoretical cross‐link density of the nanofibrous scaffolds

“…Therefore, the nanofibers from gelatin/cellulose acetate (CA) with 0 to 10% (wt/wt) elastin were prepared using electrospinning technique. The formulations and processing condition were set according to the published research . Scaffolds were then cross‐linked using glutaraldehyde vapor at 40°C for 6 hours.…”

“…The formulations and processing condition were set according to the published research. 3 Scaffolds were then cross-linked using glutaraldehyde vapor at 40°C for 6 hours.…”