Nanofibrous scaffolds releasing a small molecule BDNF-mimetic for the re-direction of endogenous neuroblast migration in the brain

Fon, Deniece; Zhou, Kun; Ercole, Francesca; Fehr, Friederike; Marchesan, Silvia; Minter, Myles R.; Crack, Peter J; Finkelstein, David I.; Forsythe, John S.

doi:10.1016/j.biomaterials.2013.12.016

Cited by 57 publications

(42 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most common method used to rescue fiber diameter is to adjust the percentage of polymer in the solution [Fon et al, 2014; Schaub, and Gilbert, 2011]. Increasing the polymer weight percent increases the opportunity for chain entanglement, and therefore increases the viscosity of the solution.…”

Section: Drug Inclusion During Emulsion Electrospinning Alters Fiber’mentioning

confidence: 99%

Electrospun Fibers for Drug Delivery after Spinal Cord Injury and the Effects of Drug Incorporation on Fiber Properties

Johnson¹,

D’Amato²,

Gilbert³

2016

Cells Tissues Organs

View full text Add to dashboard Cite

There is currently no cure for individuals with spinal cord injury (SCI). While many promising approaches are being tested in clinical trials, the complexity of SCI limits several of these approaches from aiding complete functional recovery. Several different categories of biomaterials are investigated for their ability to guide axonal regeneration, to deliver proteins or small molecules locally, or to improve the viability of transplanted stem cells. The purpose of this study is to provide a brief overview of SCI, present the different categories of biomaterial scaffolds that direct and guide axonal regeneration, and then focus specifically on electrospun fiber guidance scaffolds. Much like other polymer guidance approaches, electrospun fibers can retain and deliver therapeutic drugs. The experimental section presents new data showing the incorporation of two therapeutic drugs into electrospun poly-L-lactic acid fibers. Two different concentrations of either riluzole or neurotrophin-3 were loaded into the electrospun fibers to examine the effect of drug concentration on the physical characteristics of the fibers (fiber alignment and fiber diameter). Overall, the drugs were successfully incorporated into the fibers and the release was related to the loading concentration. The fiber diameter decreased with the inclusion of the drug, and the decreased diameter was correlated with a decrease in fiber alignment. Subsequently, the study includes considerations for successful incorporation of a therapeutic drug without changing the physical properties of the fibers.

show abstract

Section: Drug Inclusion During Emulsion Electrospinning Alters Fiber’mentioning

confidence: 99%

Electrospun Fibers for Drug Delivery after Spinal Cord Injury and the Effects of Drug Incorporation on Fiber Properties

Johnson¹,

D’Amato²,

Gilbert³

2016

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…While neuroblasts were reported to have migrated into the gels, it is unclear how the material stimulated NSPC proliferation. Aligned PCL nanofibers were used to promote NSPC migration into the injury site; however, long-term neuroblast survival required inclusion of a BDNF-mimetic peptide (Fon et al, 2014).…”

Section: Stimulating Endogenous Stem Cellsmentioning

confidence: 99%

Harnessing the Potential of Biomaterials for Brain Repair after Stroke

2018

View full text Add to dashboard Cite

“…The main focus of research is on neurotrophic factors, which include brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Generally, neurotrophic factors are added to the scaffold material; [18][19][20] however, this method is not effective. After the peripheral nerve injury, Schwann cells transform into a nonmyelinated state, "activated state".…”

mentioning

confidence: 99%

In vitro study of neural stem cells and activated Schwann cells cocultured on electrospinning polycaprolactone scaffolds

Fan¹,

Zhou²,

Wang³

et al. 2017

View full text Add to dashboard Cite

Background:To investigate the biocompatibility of electrospinning polycaprolactone (PCL) fiber scaffolds and coculture system, which consisted of neural stem cells (NSCs) and activated Schwann cells (ASCs). Materials and methods: ASCs were isolated from sciatic nerves, ligated for 7 days, in 4-week-old Wistar rats, and the NSCs were isolated from the hippocampus of E14.5 Wistar rat embryos. ASCs, NSCs and ASCs combined with NSCs were 3D cultured on the electrospinning PCL fiber scaffolds. Crystal violet staining was used to find the suitable density of ASCs for growth, and the proliferation of NSCs and ASCs were tested by Cell Counting Kit (CCK)-8 assay, and cell adhesion, differentiation of NSCs and myelin basic protein (MBP) expression of ASCs were observed by laser confocal microscopy. Distribution and morphology were assessed by scanning electron microscopy. Results: The average diameter of fibers in electrospinning PCL scaffolds was approximately 7.93±1.41 μm. ASCs could grow well at the density of 2×10 4 /cm 2 , and a certain number of cells extended along the longitudinal axis of fibers, and the shape of the cells was spindle, which was consistent with crystal violet staining results. The CCK-8 experiment showed ASCs could proliferate gradually on the PCL scaffold within 7 days, as well as NSCs, and NSCs differentiated into astrocytes, neurons and oligodendrocytes on the PCL scaffold; PCL scaffolds could improve the differentiation rate of neurons. After NSCs and ASCs were cocultured on electrospinning PCL scaffolds, ASCs could express MBP and NSCs could differentiate into neurons, which distributed around those ASCs expressing MBP. Conclusion: Electrospinning PCL fibrous scaffolds showed good biocompatibility, and the fibers had an inducing effect on the distribution of ASCs. NSCs and ASCs cultured on electrospinning PCL scaffolds could form 3D culture system, and NSCs could differentiate into neurons which distributed around the ASCs expressing MBP.

show abstract

Nanofibrous scaffolds releasing a small molecule BDNF-mimetic for the re-direction of endogenous neuroblast migration in the brain

Cited by 57 publications

References 76 publications

Electrospun Fibers for Drug Delivery after Spinal Cord Injury and the Effects of Drug Incorporation on Fiber Properties

Electrospun Fibers for Drug Delivery after Spinal Cord Injury and the Effects of Drug Incorporation on Fiber Properties

Harnessing the Potential of Biomaterials for Brain Repair after Stroke

In vitro study of neural stem cells and activated Schwann cells cocultured on electrospinning polycaprolactone scaffolds

Contact Info

Product

Resources

About