Nanofibrous membranes for single-step immobilization of hyperthermophilic enzymes

Tang, Christina; Saquing, Carl D.; Sarin, Pooja K.; Kelly, Robert M.; Khan, Saad A.

doi:10.1016/j.memsci.2014.08.037

Cited by 29 publications

(11 citation statements)

References 75 publications

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“…The above disadvantages can be overcome by enzyme immobilization. It is currently considered the best and most common method of modifying enzyme properties (Forsyth and Patwardhan 2013 ; Sheldon 2007 ; Tang et al 2014 ). Despite the huge diversity of strategies, the biocatalyst immobilization is still connected with some inconveniences.…”

Section: Introductionmentioning

confidence: 99%

Controlling enzymatic activity by immobilization on graphene oxide

Bolibok

Wiśniewski

Roszek

et al. 2017

Sci Nat

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In this study, graphene oxide (GO) has been applied as a matrix for enzyme immobilization. The protein adsorption capacity of GO is much higher than of other large surface area carbonaceous materials. Its structure and physicochemical properties are reported beneficial also for enzymatic activity modifications. The experimental proof was done here that GO-based biocatalytic systems with immobilized catalase are modifiable in terms of catalyzed reaction kinetic constants. It was found that activity and stability of catalase, considered here as model enzyme, closely depend on enzyme/GO ratio. The changes in kinetic parameters can be related to secondary structure alterations. The correlation between enzyme/GO ratio and kinetic and structure parameters is reported for the first time and enables the conscious control of biocatalytic processes and their extended applications. The biological activity of obtained biocatalytic systems was confirmed in vitro by the use of functional test. The addition of immobilized catalase improved the cells’ viability after they were exposed to hydrogen peroxide and tert-butyl-hydroperoxide used as source of reactive oxygen species.Electronic supplementary materialThe online version of this article (doi:10.1007/s00114-017-1459-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Controlling enzymatic activity by immobilization on graphene oxide

Bolibok

Wiśniewski

Roszek

et al. 2017

Sci Nat

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“…In the last two decades, polymer fibers and membranes had undergone through significant progress in the field of biomaterials engineering and biotechnology (Chen et al 2009;Hong et al 2013;Lu et al 2014;Tang et al 2014). Having applications in areas such as tissue engineering, controlled drug release, wound dressings, molecular separation, preservation of bioactive agents and biosensors have drawn a great deal of importance in fiber developments (Luo et al 2010;Zhang et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors

Hosseini

Azari

Farahmand

et al. 2015

Biosensors and Bioelectronics

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“…Membranes used in MBRs definitely play key roles in membrane fouling. Some novel membranes or membrane devices have been reported [8,9]. Sludge suspension is a collection of various matters where sludge flocs account for the largest proportion of biomass [6,10].…”

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confidence: 99%