Nanofibrous Membrane with Encapsulated Glucose Oxidase for Self-Sustained Antimicrobial Applications

Abstract

Polyvinyl alcohol (PVA) nanofibrous membrane, consisting of separately encapsulated glucose oxidase (GOx) and glucose (Glu) nanofibers, was prepared via simultaneously electrospinning PVA/GOx and PVA/Glu dopes. The as-prepared pristine membrane could self-sustainably generate hydrogen peroxide (H2O2) only in contact with an aqueous solution. The H2O2 production level was well maintained even after storing the dry membrane at room temperature for 7 days. Cross-linking the membrane via reaction with glutaraldehyde (GA) vapor could not only prevent the nanofibrous membrane from dissolving in water but also prolonged the release of H2O2. The sustained release of H2O2 from the membrane achieved antimicrobial capability equivalent to that of 1% H2O2 against both Escherichia coli and Staphylococcus aureus. Gram(+) S. aureus cells were more susceptible to H2O2 than Gram(−) E. coli and >99% of S. aureus were killed after 1 h incubation with the membrane. Pristine and GA-crosslinked nanofibrous membrane with in situ production of H2O2 were self-sterilized in which no microorganism contamination on the membrane could be detected after 2 weeks incubation on an agar plate. The GOx/Glu membrane may find potential application as versatile antimicrobial materials in the field of biomedicine, in the food and health industries, and especially challenges related to wound healing in diabetic patients.

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

“…In contrast, shown in the right column of Figure 1 , water-insoluble and bio-degradable polymers are often exploited to prepare casting films for providing drug sustained release profiles [ 37 , 38 , 39 , 40 , 41 ]. As the polymeric carriers are insoluble in water, the loaded drug molecules must be freed into the dissolution medium through a diffusion mechanism [ 42 , 43 , 44 , 45 ]. This is also the dominant mechanism for many drug molecules released from their inorganic carriers, such as carbon nano tubes, silicon dioxide, and little molecules such as lipids [ 46 , 47 , 48 , 49 ].…”

Novel Polyamide/Chitosan Nanofibers Containing Glucose Oxidase and Rosemary Extract: Fabrication and Antimicrobial Functionality

“…In general, the activity of GOX as an enzyme varies based on certain circum such as pH and the existence of different inhibitors. Previous studies suggested si heavy metals as the inhibitors for the GOX activity [47]. To this end, we exam enzymatic activity of the MBN-Ag/CS nanofiber composites (ratios at 40:60, 50:5 70:30, and 80:20) containing 0.03 mg of GOX, and the results are presented in Figu can be seen that the GOX activity was decreased by increasing the ratio of MBN-A In this study, MBN-Ag material contained silver particles, as received from the m turer.…”

“…In general, the activity of GO x as an enzyme varies based on certain circumstances, such as pH and the existence of different inhibitors. Previous studies suggested silver and heavy metals as the inhibitors for the GO x activity [47]. To this end, we examined the enzymatic activity of the MBN-Ag/CS nanofiber composites (ratios at 40:60, 50:50, 60:40, 70:30, and 80:20) containing 0.03 mg of GO x , and the results are presented in Figure 11.…”

Combination of Enzymes with Materials to Give Them Antimicrobial Features: Modern Trends and Perspectives

“…One of the current trends in the development of a strategy for combating bacterial contaminations and creating combined antimicrobial materials is focused on the use of different enzymes [ 8 , 9 , 10 ]. To obtain antibacterial materials, enzymes are used both individually and in combination with other enzymes, antimicrobial peptides, antibiotics, metal nanoparticles, etc.…”