Nanofibers of gelatin and polivinyl-alcohol-chitosan for wound dressing application: fabrication and characterization

Campa-Siqueiros, Paola I.; Madera-Santana, Tomás J.; Ayala‐Zavala, J. Fernando; López‐Cervantes, Jaime; Castillo‐Ortega, M. M.; Herrera‐Franco, Pedro J.

doi:10.1590/0104-1428.07919

Cited by 22 publications

(13 citation statements)

References 44 publications

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“…A second endotherm peak corresponding to the crystalline melting point (T m ) of PVA was observed at 210 • C. The degree of crystallinity of PVA 1Y was estimated to be 30% and was determined as the ratio between the calculated heat of fusion (∆H f ; 47 J g −1 ) and the thermodynamic enthalpy of melting of a 100% crystalline PVA (∆H 0 ; 150 J g −1 ) [31]. The third and fourth peaks at 330 and 370 • C are related to the PVA degradation of the C-C main chain, the elimination of hydroxyl groups as water, and the formation of polyene macromolecules [32]. However, the presence of two expressive peaks at such close temperatures could indicate the presence of crosslinked side-chain degradation [33,34].…”

Section: Dtamentioning

confidence: 99%

Thermal, Mechanical and Chemical Analysis of Poly(vinyl alcohol) Multifilament and Braided Yarns

et al. 2021

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Poly(vinyl alcohol) (PVA) in multifilament and braided yarns (BY) forms presents great potential for the design of numerous applications. However, such solutions fail to accomplish their requirements if the chemical and thermomechanical behaviour is not sufficiently known. Hence, a comprehensive characterisation of PVA multifilament and three BY architectures (6, 8, and 10 yarns) was performed involving the application of several techniques to evaluate the morphological, chemical, thermal, and mechanical features of those structures. Scanning electron microscopy (SEM) was used to reveal structural and morphological information. Differential thermal analysis (DTA) pointed out the glass transition temperature of PVA at 76 °C and the corresponding crystalline melting point at 210 °C. PVA BY exhibited higher tensile strength under monotonic quasi-static loading in comparison to their multifilament forms. Creep tests demonstrated that 6BY structures present the most deformable behaviour, while 8BY structures are the least deformable. Relaxation tests showed that 8BY architecture presents a more expressive variation of tensile stress, while 10BY offered the least. Dynamic mechanical analysis (DMA) revealed storage and loss moduli curves with similar transition peaks for the tested structures, except for the 10BY. Storage modulus is always four to six times higher than the loss modulus.

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Section: Dtamentioning

confidence: 99%

Thermal, Mechanical and Chemical Analysis of Poly(vinyl alcohol) Multifilament and Braided Yarns

et al. 2021

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“…Even though the hydrophobic behavior of CH films has been reported in other works, with WCAs > 88 • [79,80], the blending with PVA, with very hydrophilic nature and WCAs < 10 • [81], affects the wettability of the nanolayer produced. Furthermore, the decrease in the WCA with increasing PVA content in a PVA_CH blend has been previously described [79,82].…”

Section: Water Contact Angle Analysismentioning

confidence: 83%

Antimicrobial Food Packaging Based on Prodigiosin-Incorporated Double-Layered Bacterial Cellulose and Chitosan Composites

et al. 2022

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Nowadays, food packaging systems have shifted from a passive to an active role in which the incorporation of antimicrobial compounds into biopolymers can promote a sustainable way to reduce food spoilage and its environmental impact. Accordingly, composite materials based on oxidized-bacterial cellulose (BC) and poly(vinyl alcohol)-chitosan (PVA-CH) nanofibers were produced by needleless electrospinning and functionalized with the bacterial pigment prodigiosin (PG). Two strategies were explored, in the first approach PG was incorporated in the electrospun PVA-CH layer, and TEMPO-oxidized BC was the substrate for nanofibers deposition (BC/PVA-CH_PG composite). In the second approach, TEMPO-oxidized BC was functionalized with PG, and afterward, the PVA-CH layer was electrospun (BC_PG/PVA-CH composite). The double-layer composites obtained were characterized and the nanofibrous layers displayed smooth fibers with average diameters of 139.63 ± 65.52 nm and 140.17 ± 57.04 nm, with and without pigment incorporation, respectively. FTIR-ATR analysis confirmed BC oxidation and revealed increased intensity at specific wavelengths, after pigment incorporation. Moreover, the moderate hydrophilic behavior, as well as the high porosity exhibited by each layer, remained mostly unaffected after PG incorporation. The composites’ mechanical performance and the water vapor transmission rate (WVTR) evaluation indicated the suitability of the materials for certain food packaging solutions, especially for fresh products. Additionally, the red color provided by the bacterial pigment PG on the external surface of a food packaging material is also a desirable effect, to attract the consumers’ attention, creating a multifunctional material. Furthermore, the antimicrobial activity was evaluated and, PVA-CH_PG, and BC_PG layers exhibited the highest antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Thus, the fabricated composites can be considered for application in active food packaging, owing to PG antimicrobial potential, to prevent foodborne pathogens (with PG incorporated into the inner layer of the food packaging material, BC/PVA-CH_PG composite), but also to prevent external contamination, by tackling the exterior of food packaging materials (with PG added to the outer layer, BC_PG/PVA-CH composite).

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“…[ 60 ] The PVA nanofibrous web was the smallest contact angle because of the existence of OH groups. [ 20,60 ] The contact angle of nanofibrous webs was reduced by decreasing the chitosan:gelatin ratios from 75:25 to 25:75, which attributed to the highly hydrophilic nature of gelatin as well as the relatively hydrophobic nature of chitosan. [ 20,59 ] Naghavi Alhosseini et al.…”

Section: Resultsmentioning

confidence: 99%

Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Hadipour-Goudarzi

Hemmatinejad

Shokrgozar

2023

Macro Materials & Eng

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Chitosan/gelatin‐based nanofibers display excellent biological performance in tissue engineering because of their biocompatible composition and nanofibrous structure with a high surface‐to‐volume ratio mimicking the native extracellular matrix. In this study, to save time and cost of experiments, a response surface methodology based on Box–Behnken design (BBD) is developed to predict the mean diameter of (chitosan:gelatin)/poly(vinyl alcohol) (PVA) nanofibers in three volume ratios of chitosan:gelatin by considering PVA percentage, applied voltage, and flow rate as input variables. The morphology and chemical composition of nanofibers are investigated through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR), respectively. The optimum conditions to yield the minimum diameter of nanofibers with chitosan:gelatin ratios of 25:75, 50:50, and 75:25 are found and result in 165, 121, and 92 nm, respectively, which show good accordance with BBD estimated results. The tensile testing indicates that nanofibers containing higher ratio of chitosan:gelatin result in higher tensile stress and lower toughness and tensile strain. The water contact angle analysis (WCA) shows the appropriate hydrophilicity of crosslinked nanofibers. The MTT assay shows excellent cell viability and cell attachment of nanofibers for mouse fibroblast (L929) cells. The results indicate that optimum nanofibers are potent candidates for wound healing applications.

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Nanofibers of gelatin and polivinyl-alcohol-chitosan for wound dressing application: fabrication and characterization

Cited by 22 publications

References 44 publications

Thermal, Mechanical and Chemical Analysis of Poly(vinyl alcohol) Multifilament and Braided Yarns

Thermal, Mechanical and Chemical Analysis of Poly(vinyl alcohol) Multifilament and Braided Yarns

Antimicrobial Food Packaging Based on Prodigiosin-Incorporated Double-Layered Bacterial Cellulose and Chitosan Composites

Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

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