Nanofiber Scaffolds Support Bone Regeneration Associated with Pulp Stem Cells

Acasigua, Gerson A.; Bernardi, Lisiane; Braghirolli, Daikelly Iglesias; Filho, Manoel Sant’Ana; Pranke, Patrícia; Fossati, Anna Christina Medeiros

doi:10.2174/1574888x09666140228123911

Cited by 23 publications

(29 citation statements)

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“…Animals transplanted with SHEDs received 3×10 5 cells diluted in 0.9% NaCl. A 10-µL cell suspension was injected at the lesion site 1 h after the injury with a 25-µL sterile Hamilton syringe, and carried out without immunosuppression ( 18 ). Following the SCI procedure, animals were sutured and housed in individual cages and bladder evacuation was performed daily until they recover the function.…”

Section: Methodsmentioning

confidence: 99%

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury

Nicola

Rodrigues

Crestani

et al. 2016

Braz J Med Biol Res

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Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-α levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-α levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action.

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Section: Methodsmentioning

confidence: 99%

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury

Nicola

Rodrigues

Crestani

et al. 2016

Braz J Med Biol Res

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“…Implantation of human DSCs seeded in beta-tri-calcium phosphate scaffolds exerted an anti-inflammatory effect and restored periodontal hard tissue defects[ 163 ]. Greater bone regeneration was also reported when human DSCs were seeded on poly-lactic-co-glycolic acid[ 164 ] and α- calcium sulfate hemihydrate/amorphous calcium phosphate[ 165 ] scaffolds. Calcium phosphate cement functionalized with iron oxide nanoparticles also exhibits a potent effect on the spreading, osteogenic differentiation, and bone mineral synthesis of DSCs, possibly via activation of the extracellular signal-related kinases WNT/β-catenin pathway[ 166 ].…”

Section: Bone Reconstructionmentioning

confidence: 99%

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migration, and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific purposes. An enormous variety of scaffolds have been used for tissue engineering with DSCs. Of these, the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis, cell-matrix interactions, degradation of extracellular matrix, organized matrix formation, and the mineralization abilities of DSCs in both in vitro and in vivo conditions. DSCs represent a promising cell source for tissue engineering, especially for tooth, bone, and neural tissue restoration. The purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

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“…In this study, the expression of collagen fibers was compared with the findings on bone formation of a previously published paper 10 , which found that the association of scaffolds and osteogenic stimulated stem cells promoted higher bone formation than the control group. The same pattern was observed in our data, in which the group of stimulated stem cells showed more total collagen formation than the other groups.…”

Section: Discussionmentioning

confidence: 99%

“…A possible explanation to this observation is that due to the local inflammatory process there was a delay in the differentiation of stem cells into osteoblasts and a consequent decrease in collagen formation. On the other hand, the scaffold itself worked as a framework for the cell environment assisting the healing process, as described by Bottaro 17 10 . This study found a significant amount of bone neoformation in bone defects of mice, which had been implanted with stem cells previously differentiated into an osteogenic medium and a PLGA scaffold.…”

Section: Discussionmentioning

confidence: 99%

“…Nanofiber scaffolds ( Figure 1) were prepared according to a previously published method 10 . A polymeric solution of poly(D,L-acid lactic-co-glycolic acid) (75:25) (PLGA) (Sigma Aldrich, St Louis, MO, USA) was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (Sigma Aldrich) until the final concentration of the solution reached 12% (w/v), and it was maintained in a magnetic stirrer for 24 hours.…”

Section: Nanofiber Scaffoldsmentioning

confidence: 99%

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Collagenous matrix as a predictor for bone formation: a digital technique for collagen quantification

Acasigua

Quevedo

Fossati

2017

Rev. da Fac. de Odontologia, UPF

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The collagenous matrix plays a fundamental role in the process of bone regeneration, so it is essential to study how it is primarily formed in situations in which critical bone defects are created. Objective: this study seeks to quantify the collagenous matrix formed in critical bone defects in the calvaria of mice over the process of bone regeneration promoted by the association of poly(lactide-co-glycolide) (PLGA) porous scaffolds and stem cells from deciduous teeth (SCDT). In addition, this study attempted to establish a precise protocol for the digital quantification of collagen through a histological method. Materials and method: Nine Wistar rats were used, in which critical defects of 8.0 mm of diameter were made in their calvarium. The animals were divided into three groups (n = 9): I – PLGA scaffolds; II – PLGA scaffolds/SCDT; III – PLGA scaffolds/SCDT maintained in osteogenic medium for 13 days. Within sixty postoperative days, calvaria were removed for histometric analysis following a digital protocol. A specific digital analysis method was designed for this study, in which a more precise quantification and differentiation between collagen fibers and non-collagenous tissue was possible, excluding factors that would normally alter the results. Results: it was noted that the association of PLGA scaffolds and SCDT maintained in osteogenic medium resulted in collagen matrix formation statistically higher than the other groups (p

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Nanofiber Scaffolds Support Bone Regeneration Associated with Pulp Stem Cells

Cited by 23 publications

References 0 publications

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Collagenous matrix as a predictor for bone formation: a digital technique for collagen quantification

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