Nanofiber garlands of polycaprolactone by electrospinning

Reneker, Darrell H.; Kataphinan, W.; Théron, A.; Zussman, Eyal; Yarin, Alexander L.

doi:10.1016/s0032-3861(02)00595-5

Cited by 222 publications

(154 citation statements)

References 22 publications

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“…Under specific operation conditions, which are not typical for the process, it is observed that joining loops and fragmentation of fibers occurs leading to the formation of irregular shapes of fibers. 52 Applied modeling of the process was developed in the recent years 53 54 but an engineering model that can enable process control has not been established yet. The process may occur either at room temperature or inside a fume chamber depending on the used polymer and solvent type.…”

Section: Ashammakhi Et Almentioning

confidence: 99%

Biodegradable Nanomats Produced by Electrospinning: Expanding Multifunctionality and Potential for Tissue Engineering

Ashammakhi

Ndreu²,

Piras³

et al. 2007

j nanosci nanotechnol

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With increasing interest in nanotechnology, development of nanofibers (n-fibers) by using the technique of electrospinning is gaining new momentum. Among important potential applications of n-fiber-based structures, scaffolds for tissue-engineering represent an advancing front. Nanoscaffolds (n-scaffolds) are closer to natural extracellular matrix (ECM) and its nanoscale fibrous structure. Although the technique of electrospinning is relatively old, various improvements have been made in the last decades to explore the spinning of submicron fibers from biodegradable polymers and to develop also multifunctional drug-releasing and bioactive scaffolds. Various factors can affect the properties of resulting nanostructures that can be classified into three main categories, namely: (1) Substrate related, (2) Apparatus related, and (3) Environment related factors. Developed n-scaffolds were tested for their cytocompatibility using different cell models and were seeded with cells for to develop tissue engineering constructs. Most importantly, studies have looked at the potential of using n-scaffolds for the development of blood vessels. There is a large area ahead for further applications and development of the field. For instance, multifunctional scaffolds that can be used as controlled delivery system do have a potential and have yet to be investigated for engineering of various tissues. So far, in vivo data on n-scaffolds are scarce, but in future reports are expected to emerge. With the convergence of the fields of nanotechnology, drug release and tissue engineering, new solutions could be found for the current limitations of tissue engineering scaffolds, which may enhance their functionality upon in vivo implantation. In this paper electrospinning process, factors affecting it, used polymers, developed n-scaffolds and their characterization are reviewed with focus on application in tissue engineering.

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Section: Ashammakhi Et Almentioning

confidence: 99%

Biodegradable Nanomats Produced by Electrospinning: Expanding Multifunctionality and Potential for Tissue Engineering

Ashammakhi

Ndreu²,

Piras³

et al. 2007

j nanosci nanotechnol

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“…The PCL fibril, consisting of a filament, has size of about 12 m in the direction of fiber length and cross-section of about 500 nm. Recently, Reneker and colleagues reported that a garland, 31 in the form of a long, slender, and irregular network of electrically charged nanofibers, which had a fuzzy appearance and followed a curly path, was created when solution ejected from the tip move toward the target during electrospinning. Intra-and interlayer bonding structures within the electrospun filament could be ascribed by this phenomenon.…”

Section: Filament Morphologymentioning

confidence: 99%

Novel fabricated matrix via electrospinning for tissue engineering

et al. 2004

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Electric field-driven fiber formation (electrospinning) is developing into a practical means for preparing novel porous filament with unusual structures and affordable mechanical properties. Polycaprolactone (PCL) was dissolved in solvent mixtures of methylene chloride/N,N-dimethyl formamide with ratios of 100/0, 75/25, and 50/50 (v/v) for electrospinning. The filament was formed by coagulation of the spinning solution following the well-known principle of phase separation in polymer solutions valid in other wet shaping processes. A strand of electrospun porous filament consisted of fibers ranging from 0.5 to 12 m in diameter. To evaluate the feasibility of three-dimensional fabric as scaffold matrices, the plain weave, which is the simplest of the weaves and the most common, was prepared with porous PCL filament. The growth characteristics of MCF-7 mammary carcinoma cells in the woven fabrics showed the important role of matrix microstructure in proliferation. This study has shown that woven fabrics, consisting of porous filaments via electrospinning, may be suitable candidates as tissue engineering scaffolds.

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“…Polycaprolactone (PCL), a hydrophobic polymer, is a well-known biocompatible polymer on which to grow human cells and PCL nanofiber scaffolds can be produced by electrospinning. [6][7][8][9] The nanofiber scaffolds are particularly useful because of their high surface area and porosity, which stimulates cell adhesion. Modifications and blending of PCL with other chemicals or proteins can create environments that can become even more conducive to cell growth.…”

Section: Introductionmentioning

confidence: 99%

Electrospun fiber membranes enable proliferation of genetically modified cells

Borjigin¹,

Eskridge²,

Niamat³

et al. 2013

IJN

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Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces.

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Nanofiber garlands of polycaprolactone by electrospinning

Cited by 222 publications

References 22 publications

Biodegradable Nanomats Produced by Electrospinning: Expanding Multifunctionality and Potential for Tissue Engineering

Biodegradable Nanomats Produced by Electrospinning: Expanding Multifunctionality and Potential for Tissue Engineering

Novel fabricated matrix via electrospinning for tissue engineering

Electrospun fiber membranes enable proliferation of genetically modified cells

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