Nanofat Grafting

Abstract

Nanofat injections might become a new concept in the lipofilling area. In clinical situations, nanofat seems to be suitable for skin rejuvenation purposes.

Reply: Phenotypic Analysis of Stromal Vascular Fraction after Mechanical Shear Reveals Stress-Induced Progenitor Populations

“…We have conducted internal experiments on the cellular constituency of the filtrate and filtrand of intersyringe-processed fat and observed a significant number of retained cells in the filtrand, indicating a large portion of regenerative cells that are closely intimated to the adipose extracellular matrix. Although our results are not directly comparable to the filtrate analysis performed by Tonnard et al, 2 we do not believe our results represent a completely different cell population, but rather a more complete picture of the effects of intersyringe processing on the phenotypic properties of standard lipoaspirate. In fact, we have recently presented phenotypic data of mechanically processed lipoaspirate that is more analogous to nanofat processing and observed the same populations with similar effect.…”

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

“…Nanofat is an emulsified suspension rich in SVF, which was proposed for use in anti-aging medicine for the first time by Tonnard et al in 2013 [4]. The use of nanofat has been generalized and applied for cosmetic treatment in recent years [30, 31].…”

“…However, the micromolecular structure of HA shortens the duration of its efficacy. Since the anti-aging efficacy produced by a single application of nanofat or PRF is significant [4, 37], we proposed a technique to precisely inject an intradermal nanofat-PRF mixture using nine sites for better rejuvenation (Fig. 8A).…”

“…In 2013, Tonnard et al reported on the efficiency and safety of treating skin aging with nanofat, which is different from traditional fat transplant, using intradermal injection of an emulsified fat suspension to promote skin rejuvenation [4]. Our previous research found that numerous mesenchymal stem cells (MSCs) were present in an isolated culture of nanofat and were designated nanofat-derived stem cells (NFSCs).…”

Particle size in fat graft retention: A review on the impact of harvesting technique in lipofilling surgical outcomes

“…40 Briefly, microfat particles were harvested from the abdomen using a cannula with 1-mm diameter. An amount of the microfat was sheared into finer particles using a Leur-to-Leur connector with 2 10-cc syringes.…”