Nanofabrication for the Analysis and Manipulation of Membranes

Kelly, Christopher V.; Craighead, Harold G.

doi:10.1007/s10439-011-0479-y

Cited by 6 publications

(4 citation statements)

References 102 publications

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“…Cell growth is also influenced by pore depth on metal scaffolds . Computer designed scaffolds, bioprinting and other patterning techniques offer promise for hybrid technologies which will be applicable to many clinical problems If materials can be coated with the patient's own cells, biocompatibility will be greatly enhanced . Advances in nanofabrication will also foster better understanding of cellular membrane processes and will allow nanomaterials to be combined with biologic sensing elements to make biosensors which could control hormonal or neurological functions .…”

Section: Conclusion and New Horizonsmentioning

confidence: 99%

What would surgeons like from materials scientists?

Grundfest‐Broniatowski

2013

WIREs Nanomed Nanobiotechnol

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Surgery involves the repair, resection, replacement, or improvement of body parts and functions and in numerous ways, surgery should be considered human engineering. There are many areas in which surgical materials could be improved, but surgeons are generally unaware of materials available for use, while materials scientists do not know what surgeons require. This article will review some of the areas where surgeons and materials scientists have interacted in the past and will discuss some of the most pressing problems which remain to be solved. These include better implant materials for hernia repair, breast reconstruction, the treatment of diabetes, vascular stenting and reconstruction, and electrical pacing devices. The combination of tissue engineering and nanomaterials has great potential for application to nearly every aspect of surgery. Tissue engineering will allow cells or artificial organs to be grown for specific uses while nanotechnology will help to ensure maximal biocompatibility. Biosensors will be combined with improved electrodes and pacing devices to control impaired neurological functions.

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Section: Conclusion and New Horizonsmentioning

confidence: 99%

What would surgeons like from materials scientists?

Grundfest‐Broniatowski

2013

WIREs Nanomed Nanobiotechnol

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“…Synthetic model membranes enable one to study the physicochemical and functional properties of the biological membrane in detail by mimicking the membrane structure in a controlled manner. − Substrate-supported lipid bilayers (SLBs) are an attractive platform with possibilities to apply microfabrication techniques (e.g., patterning and fluidics) and highly sensitive interfacial analytical techniques. − Here, we describe a model membrane system, in which lipid bilayer and monolayer are combined to mimic the semipermeable corrals found in the cell membrane. We generated a patterned hybrid membrane having bilayer and monolayer regions using a lithographically generated monolayer of polymerized diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)- sn -glycero-3-phosphocholine (DiynePC).…”

Section: Introductionmentioning

confidence: 99%

Retarded Diffusion and Confinement of Membrane-Bound Molecules in a Patterned Hybrid Membrane of Phospholipid Bilayers and Monolayers

et al. 2023

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The biological membrane is a complex two-dimensional fluid, in which various molecular interactions regulate the lateral diffusion of membrane-associated molecules. Pinning of membrane proteins or lipids by extra-membrane proteins impedes the diffusion. In addition, coupling between two monolayer leaflets within a phospholipid bilayer via interdigitation plays important roles, though this effect remains elusive. Here, we fabricate a substrate-supported model membrane with patterned bilayer/monolayer regions to explore the influences of interleaflet coupling. A patterned monolayer of polymerized diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), was lithographically generated and used to form patterned lipid bilayers and monolayers. A phospholipid monolayer was formed on top of the polymerized monolayer. The bilayer/monolayer hybrid membrane was continuous and fluid, but lateral diffusion in the monolayer region was significantly retarded, suggesting the influences of interleaflet coupling. We reconstituted photoreceptor rhodopsin (Rh) and G-protein transducin (Gt) as model transmembrane and peripheral proteins. Rh diffused laterally only in the bilayer region, whereas Gt diffused in both bilayer and monolayer regions. The patterned hybrid bilayer/monolayer membrane reproduces the retarded diffusion and confinement of membrane-bound molecules in a controlled manner and provides insight into the physicochemical and functional roles of semipermeable corrals in the cell membrane.

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“…13 A number of comprehensive reviews have been published on various aspects of lipid bilayers, including their formation mechanisms, characterization techniques, their regulation of membrane proteins, and their integration in biosensors and other devices. 3,11,13,14,15,16,17,18,19,20,21,22 Owing to space limitations, this review will focus exclusively on micro/nanostructured scaffolds that emulate in some way the connections of a cellular membrane to an underlying "cytoskeleton" (figure 1). These structured scaffolds could be fabricated from solid-state inorganic materials like metals, 23,24,25 semiconductors 26 or oxides, 27,28 "soft" polymeric biomolecules 29,30,31 such as PEG, collagen, or actin, or combinations of these.…”

Section: Introductionmentioning

confidence: 99%

Bilayer membrane interactions with nanofabricated scaffolds

Collier

2015

Chemistry and Physics of Lipids

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Membrane function is facilitated by lateral organization within the lipid bilayer, including phase-separation of lipids into more ordered domains (lipid rafts) and anchoring of the membrane to a cytoskeleton. These features have proven difficult to reproduce in model membrane systems such as black lipid membranes, unilamellar vesicles and supported bilayers. However, advances in micro/nanofabrication have resulted in more realistic synthetic models of membrane-cytoskeleton interactions that can help uncover the design rules responsible for biological membrane formation and organization. This review will focus on describing micro-/nanostructured scaffolds that can emulate the connections of a cellular membrane to an underlying "cytoskeleton". Examples include molecular-based scaffolds anchored to a solid substrate through surface chemistry, solid-state supports modified by material deposition, lithography and etching, the creation of micro/nanoporous arrays, integration with microfluidics, and droplet-based bilayers at interfaces.Model systems such as these are increasing our understanding of structure and organization in cell membranes, and how they result in the emergence of functionality at the nanoscale.

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Nanofabrication for the Analysis and Manipulation of Membranes

Cited by 6 publications

References 102 publications

What would surgeons like from materials scientists?

What would surgeons like from materials scientists?

Retarded Diffusion and Confinement of Membrane-Bound Molecules in a Patterned Hybrid Membrane of Phospholipid Bilayers and Monolayers

Bilayer membrane interactions with nanofabricated scaffolds

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