Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles

Tomanin, Pietro Pacchin; Zhou, Jiajing; Amodio, Alessia; Cimino, Rita; Glab, Agata; Cavalieri, Francesca; Caruso, Frank

doi:10.1039/d0tb00299b

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…[22][23] Of these natural nanoparticle systems, glycogen, a highly branched polysaccharide nanoparticle, is one of the most readily available and versatile nanoparticles. Recently, glycogen has gained attention as an advanced material for various therapeutic-based applications, [24][25] including for use as cancer targeting 26 and penetrating nanoparticles, 27 as in vivo contrast agents 28 and immunomodulators, 29 and as a component in biodegradable hydrogels, 30 films, 31 and fibers. [32][33] The intracellular synthesis of glycogen is highly regulated by various enzymatic cascades, where the biochemical pathways are uniquely adapted to the metabolic demands of each cell type, resulting in different particle structures between sources.…”

Section: Introductionmentioning

confidence: 99%

“…[38][39] From a material science perspective, glycogen can be obtained commercially from various sources 36 and modified both enzymatically and synthetically. 24 Several synthetic methods to endow glycogen with functionality include periodate oxidation with subsequent reductive amination, 27 N,N′carbonyl diimidazole activation of the hydroxyl groups followed by amide formation with primary amine-containing molecules, 30 carbodiimide-catalyzed esterification of the hydroxyl groups with carboxylate-containing molecules in the presence of a base, 31 and etherification of the hydroxyl groups with an alkylating agent in the presence of a base. 26 These synthetic methods exploit the modification of the glycosidic component of glycogen, which can influence the biodegradation 27 and immunostimulation of the particles.…”

Section: Introductionmentioning

confidence: 99%

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Protein Component of Oyster Glycogen Nanoparticles: An Anchor Point for Functionalization

Besford

Weiss

Schubert

et al. 2020

ACS Appl. Mater. Interfaces

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Bio-sourced nanoparticles have a range of desirable properties for therapeutic applications, including biodegradability and low immunogenicity. Glycogen, a natural polysaccharide nanoparticle, has garnered much interest as a component of advanced therapeutic materials. However, functionalizing glycogen for use as a therapeutic material typically involves synthetic approaches that can negatively affect the intrinsic physiological properties of glycogen. Herein, the protein component of glycogen is examined as an anchor point for the photopolymerization of functional poly(N-isopropylacrylamide) (PNIPAM) polymers. Oyster glycogen (OG) nanoparticles partially degrade to smaller spherical particles in the presence of protease enzymes, reflecting a population of surface-bound proteins on the polysaccharide. The grafting of PNIPAM to the native protein component of OG produces OG-PNIPAM nanoparticles of ~45 nm in diameter and 6.2 MDa in molecular weight. PNIPAM endows the nanoparticles with temperatureresponsive aggregation properties that are controllable, reversible, and which can be removed by the biodegradation of the protein. The OG-PNIPAM nanoparticles retain the native biodegradability of glycogen. Whole blood incubation assays revealed that the OG-PNIPAM nanoparticles have a low cell association and inflammatory response similar to that of OG. The reported strategy provides functionalized glycogen nanomaterials that retain their inherent biodegradability and low immune cell association.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein Component of Oyster Glycogen Nanoparticles: An Anchor Point for Functionalization

Besford

Weiss

Schubert

et al. 2020

ACS Appl. Mater. Interfaces

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“…(G) Schematic of the L–PG assembly on a substrate and co‐assembly of metal nanoparticles on the L–PG layer to obtain hybrid surfaces via layer‐by‐layer assembly for catalysis and sensing applications (Tomanin et al., 2020). (H) Water contact angles of the bare, PG‐, and phytoglycogen nanoparticles conjugated with lipoate (L–PG)‐coated substrates, emission intensities of fluorescently labeled L–PG coatings on glass substrates after incubation in different chemical and biochemical environments, and photographs of L–PG/AuNP layer‐by‐layer assemblies on various substrates (scale bar 1 cm) (Tomanin et al., 2020). (I) Catalysis and sensing applications of L–PG/metal nanoparticle layer‐by‐layer assemblies (Tomanin et al., 2020).…”

Section: Potential Applicationsmentioning

confidence: 99%

Advanced dendritic glucan‐derived biomaterials: From molecular structure to versatile applications

Chen

Zhao

McClements

et al. 2023

Comp Rev Food Sci Food Safe

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There is considerable interest in the development of advanced biomaterials with improved or novel functionality for diversified applications. Dendritic glucans, such as phytoglycogen and glycogen, are abundant biomaterials with highly branched three‐dimensional globular architectures, which endow them with unique structural and functional attributes, including small size, large specific surface area, high water solubility, low viscosity, high water retention, and the availability of numerous modifiable surface groups. Dendritic glucans can be synthesized by in vivo biocatalysis reactions using glucosyl‐1‐phosphate as a substrate, which can be obtained from plant, animal, or microbial sources. They can also be synthesized by in vitro methods using sucrose or starch as a substrate, which may be more suitable for large‐scale industrial production. The large numbers of hydroxyl groups on the surfaces of dendritic glucan provide a platform for diverse derivatizations, including nonreducing end, hydroxyl functionalization, molecular degradation, and conjugation modifications. Due to their unique physicochemical and functional attributes, dendritic glucans have been widely applied in the food, pharmaceutical, biomedical, cosmetic, and chemical industries. For instance, they have been used as delivery systems, adsorbents, tissue engineering scaffolds, biosensors, and bioelectronic components. This article reviews progress in the design, synthesis, and application of dendritic glucans over the past several decades.

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“…The outer surface of GNPs is highly branched and has a dense inner core. Because of their high hydrophilicity and biocompatibility, they can easily be biochemically modified to produce functional derivatives [ 198 ], such as adhesive GNPs composed of lipoate-conjugated phytoglycogen (L-PG) [ 199 ] and oyster GNPs with poly(N-isopropylacrylamide) (PNIPAM) chains on their surface [ 200 ].…”

Section: Np Delivery—a Platform Ensuring the Efficacy And Stability O...mentioning

confidence: 99%

Nanoparticle Delivery Platforms for RNAi Therapeutics Targeting COVID-19 Disease in the Respiratory Tract

Zhang

Almazi

Ong

et al. 2022

IJMS

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Since December 2019, a pandemic of COVID-19 disease, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread across the globe. At present, the Food and Drug Administration (FDA) has issued emergency approval for the use of some antiviral drugs. However, these drugs still have limitations in the specific treatment of COVID-19, and as such, new treatment strategies urgently need to be developed. RNA-interference-based gene therapy provides a tractable target for antiviral treatment. Ensuring cell-specific targeted delivery is important to the success of gene therapy. The use of nanoparticles (NPs) as carriers for the delivery of small interfering RNA (siRNAs) to specific tissues or organs of the human body could play a crucial role in the specific therapy of severe respiratory infections, such as COVID-19. In this review, we describe a variety of novel nanocarriers, such as lipid NPs, star polymer NPs, and glycogen NPs, and summarize the pre-clinical/clinical progress of these nanoparticle platforms in siRNA delivery. We also discuss the application of various NP-capsulated siRNA as therapeutics for SARS-CoV-2 infection, the challenges with targeting these therapeutics to local delivery in the lung, and various inhalation devices used for therapeutic administration. We also discuss currently available animal models that are used for preclinical assessment of RNA-interference-based gene therapy. Advances in this field have the potential for antiviral treatments of COVID-19 disease and could be adapted to treat a range of respiratory diseases.

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Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles

Abstract: Amphiphilic phytoglycogen nanoparticles are used as building blocks for engineering multifunctional hybrid films with catalytic and sensing properties.

Cited by 11 publications

References 35 publications

Protein Component of Oyster Glycogen Nanoparticles: An Anchor Point for Functionalization

Protein Component of Oyster Glycogen Nanoparticles: An Anchor Point for Functionalization

Advanced dendritic glucan‐derived biomaterials: From molecular structure to versatile applications

Nanoparticle Delivery Platforms for RNAi Therapeutics Targeting COVID-19 Disease in the Respiratory Tract

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