Nanoengineering bactericidal nitric oxide therapies

Cabrales, Pedro

doi:10.4161/viru.2.3.16746

Cited by 7 publications

(6 citation statements)

References 14 publications

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“…Their therapeutic use in combat and/or disaster situations in which specialized medical care or technology is not readily available would be ideal given the breadth of physiologic, and importantly, antimicrobial, activities. 17 NO-nps are an innovative approach and promising solution to the treatment of SSTI in the setting of escalating bacterial resistance.…”

Section: Discussionmentioning

confidence: 99%

“…NO has several intrinsic antimicrobial properties and is therefore vital to the body's innate immune response in the defense against invading microbes . One of the main mechanisms is its ability to generate RNOS via spontaneous reactions with oxygen or superoxide.…”

Section: Nitric Oxidementioning

confidence: 99%

“…NO is endogenously synthesized when one of three distinct nitric oxide synthase (NOS) enzymes induces the oxidation of arginine to citrulline (Figure 1). 15,17,[19][20][21][22] Two NOS isoforms, NOS1 and NOS3, are constitutively expressed 4,15,18,22,23 and are also known by the cell types in which they were enzymatically identified: NOS1 or neuronal NOS (nNOS) from neuronal cells, and NOS3 or endothelial NOS (eNOS), from endothelial cells. Both eNOS and nNOS are calcium-dependent, calmodulinregulated enzymes, 23,24 meaning their activity is tightly regulated.…”

Section: Structure and Chemical Propertiesmentioning

confidence: 99%

“…NO has several intrinsic antimicrobial properties and is therefore vital to the body's innate immune response in the defense against invading microbes. 17,18,24,26,31 One of the main mechanisms is its ability to generate RNOS via spontaneous reactions with oxygen or superoxide. These RNOS include peroxynitrite (OONO − ), RSNOs, nitrogen dioxide (NO 2 ), dinitrogen trioxide (N 2 O 3 − ), and dinitrogen tetroxide (N 2 O 4 ).…”

Section: Antimicrobial Properties and Immune Functionmentioning

confidence: 99%

“…Because of its lipophilic character and low molecular weight, NO traverses most physiologic barriers with relative ease to reach target cells . Additionally, NO diffuses along its concentration gradient and can therefore cross cell membranes without the need for transport proteins . NO is a natural yet free radical‐forming gas and is highly unstable in an oxygen environment: it spontaneously reacts with oxygen or superoxide, forming reactive nitrogen oxide species (RNOS).…”

Section: Nitric Oxidementioning

confidence: 99%

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Use of nitric oxide nanoparticulate platform for the treatment of skin and soft tissue infections

Kutner

Friedman

2013

WIREs Nanomed Nanobiotechnol

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The incidence of skin and soft tissue infections (SSTI) due to multi-drug resistant pathogens is increasing. The concomitant increase in antibiotic use along with the ease with which organisms develop mechanisms of resistance have together become a medical crisis, underscoring the importance of developing innovative and effective antimicrobial strategies. Nitric oxide (NO) is an endogenously produced molecule with many physiologic functions, including broad spectrum antimicrobial activity and immunomodulatory properties. The risk of resistance to NO is minimized because NO has multiple mechanisms of antimicrobial action. NO's clinical utility has been limited largely because it is highly reactive and lacks appropriate vehicles for storage and delivery. To harness NO's antimicrobial potential, a variety exogenous NO delivery platforms have been developed and evaluated, yet limitations preclude their use in the clinical setting. Nanotechnology represents a paradigm through which these limitations can be overcome, allowing for the encapsulation, controlled release, and focused delivery of NO for the treatment of SSTI.

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Section: Discussionmentioning

confidence: 99%

Section: Nitric Oxidementioning

confidence: 99%

Section: Structure and Chemical Propertiesmentioning

confidence: 99%

Section: Antimicrobial Properties and Immune Functionmentioning

confidence: 99%

Section: Nitric Oxidementioning

confidence: 99%

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Use of nitric oxide nanoparticulate platform for the treatment of skin and soft tissue infections

Kutner

Friedman

2013

WIREs Nanomed Nanobiotechnol

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The Use of Nanoparticles to Deliver Nitric Oxide to Hepatic Stellate Cells for Treating Liver Fibrosis and Portal Hypertension

Duong

Dong

et al. 2015

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101

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Polymeric nanoparticles are designed to transport and deliver nitric oxide (NO) into hepatic stellate cells (HSCs) for the potential treatment of both liver fibrosis and portal hypertension. The nanoparticles, incorporating NO donor molecules (S-nitrosoglutathione compound), are designed for liver delivery, minimizing systemic delivery of NO. The nanoparticles are decorated with vitamin A to specifically target HSCs. We demonstrate, using in vitro and in vivo experiments, that the targeted nanoparticles are taken up specifically by rat primary HSCs and the human HSC cell line accumulating in the liver. When nanoparticles, coated with vitamin A, release NO in liver cells, we find inhibition of collagen I and α-smooth muscle actin (α-SMA), fibrogenic genes associated with activated HSCs expression in primary rat liver and human activated HSCs without any obvious cytotoxic effects. Finally, NO-releasing nanoparticles targeted with vitamin A not only attenuate endothelin-1 (ET-1) which elicites HSC contraction but also acutely alleviates haemodynamic disorders in bile duct-ligated-induced portal hypertension evidenced by decreasing portal pressure (≈20%) and unchanging mean arterial pressure. This study clearly shows, for the first time, the potential for HSC targeted nanoparticle delivery of NO as a treatment for liver diseases with proven efficacy for alleviating both liver fibrosis and portal hypertension.

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State of the art, challenges and perspectives in the design of nitric oxide-releasing polymeric nanomaterials for biomedical applications

Seabra

Justo

Haddad

2015

Biotechnology Advances

136

105

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Nanoengineering bactericidal nitric oxide therapies

Cited by 7 publications

References 14 publications

Use of nitric oxide nanoparticulate platform for the treatment of skin and soft tissue infections

Use of nitric oxide nanoparticulate platform for the treatment of skin and soft tissue infections

The Use of Nanoparticles to Deliver Nitric Oxide to Hepatic Stellate Cells for Treating Liver Fibrosis and Portal Hypertension

State of the art, challenges and perspectives in the design of nitric oxide-releasing polymeric nanomaterials for biomedical applications

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