Nanoengineered Surfaces for Focal Adhesion Guidance Trigger Mesenchymal Stem Cell Self-Organization and Tenogenesis

Iannone, Maria; Ventre, Maurizio; Formisano, Lucia; Casalino, Laura; Patriarca, Eduardo J.; Netti, Paolo A.

doi:10.1021/nl503737k

Cited by 49 publications

(36 citation statements)

References 30 publications

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“…VitC influences ECM composition/structure, and it is now evident that the mechanical features of the ECM influence normal and cancer stem cell behavior [88–93]. For instance, while soft substrates (0.6 kPa polyacrylamide gels coated with of type-1 collagen) sustain embryonic stem cell (ESC) self-renewal [94], rigid/stiff substrates induce differentiation/lineage specification of mesenchymal stem cells (MSCs) [95].…”

Section: Vitc-dependent Regulation Of Ecm/collagen Homeostasismentioning

confidence: 99%

Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics

D’Aniello

Cermola

Patriarca

et al. 2017

Stem Cells International

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Transcription factors and signaling molecules are well-known regulators of stem cell identity and behavior; however, increasing evidence indicates that environmental cues contribute to this complex network of stimuli, acting as crucial determinants of stem cell fate. l-Ascorbic acid (vitamin C (VitC)) has gained growing interest for its multiple functions and mechanisms of action, contributing to the homeostasis of normal tissues and organs as well as to tissue regeneration. Here, we review the main functions of VitC and its effects on stem cells, focusing on its activity as cofactor of Fe+2/αKG dioxygenases, which regulate the epigenetic signatures, the redox status, and the extracellular matrix (ECM) composition, depending on the enzymes' subcellular localization. Acting as cofactor of collagen prolyl hydroxylases in the endoplasmic reticulum, VitC regulates ECM/collagen homeostasis and plays a key role in the differentiation of mesenchymal stem cells towards osteoblasts, chondrocytes, and tendons. In the nucleus, VitC enhances the activity of DNA and histone demethylases, improving somatic cell reprogramming and pushing embryonic stem cell towards the naive pluripotent state. The broad spectrum of actions of VitC highlights its relevance for stem cell biology in both physiology and disease.

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Section: Vitc-dependent Regulation Of Ecm/collagen Homeostasismentioning

confidence: 99%

Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics

D’Aniello

Cermola

Patriarca

et al. 2017

Stem Cells International

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“…69 Based on these observations, we hypothesized that nanopatterned surfaces could guide the self-organization process of human MSCs in vitro and -possibly-tissuegenesis. 70 Human MSCs were cultivated on nanograted PDMS substrates with 700 nm wide ridges, 250 nm high ( Figure 4A). In 1 week cultures, cells selforganized in the form of 3D long and thin stripes invariably oriented orthogonally to the pattern 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 30 direction ( Figure 4B).…”

Section: Effects Of Materials Features On Adhesion Proliferation and Mmentioning

confidence: 99%

Engineering Cell Instructive Materials To Control Cell Fate and Functions through Material Cues and Surface Patterning

Ventre

Netti

2016

ACS Appl. Mater. Interfaces

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107

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Mastering the interaction between cells and extracellular environment is a fundamental prerequisite in order to engineer functional biomaterial interfaces able to instruct cells with specific commands. Such advanced biomaterials might find relevant application in prosthesis design, tissue engineering, diagnostics and stem cell biology. Because of the highly complex, dynamic, and multifaceted context, a thorough understanding of the cell-material crosstalk has not been achieved yet; however, a variety of material features including biological cues, topography, and mechanical properties have been proved to impact the strength and the nature of the cell-material interaction, eventually affecting cell fate and functions. Although the nature of these three signals may appear very different, they are equated by their participation in the same material-cytoskeleton crosstalk pathway as they regulate cell adhesion events. In this work we present recent and relevant findings on the material-induced cell responses, with a particular emphasis on how the presentation of biochemical/biophysical signals modulates cell behavior. Finally, we summarize and discuss the literature data to draw out unifying elements concerning cell recognition of and reaction to signals displayed by material surfaces.

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“…[18][19][20] However, studies related on dynamic pattern writing/erasing with living cells are lacking. Based on our previous experience on cell response to static micro-and nanoscale patterns, [21][22][23] we explored the possibility of using light sensitive substrates in order to move toward the development of surfaces on which patterned signals can be manipulated dynamically.…”

Section: Introductionmentioning

confidence: 99%

Reversible Holographic Patterns on Azopolymers for Guiding Cell Adhesion and Orientation

Rianna

Calabuig

Ventre

et al. 2015

ACS Appl. Mater. Interfaces

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Topography of material surfaces is known to influence cell behavior at different levels: from adhesion up to differentiation. Different micro- and nanopatterning techniques have been employed to create patterned surfaces to investigate various aspects of cell behavior, most notably cellular mechanotransduction. Nevertheless, conventional techniques, once implemented on a specific substrate, fail in allowing dynamic changes of the topographic features. Here we investigated the response of NIH-3T3 cells to reversible topographic signals encoded on light-responsive azopolymer films. Switchable patterns were fabricated by means of a well-established holographic setup. Surface relief gratings were realized with Lloyd's mirror system and erased with circularly polarized or incoherent light. Cell cytoskeleton organization and focal adhesion assembly proved to be very sensitive to the underlying topographic signal. Thereafter, pattern reversibility was tested in air and wet environment by using temperature or light as a trigger. Additionally, pattern modification was dynamically performed on substrates with living cells. This study paves the way toward an in situ and real-time investigation of the material-cytoskeleton crosstalk caused by the intrinsic properties of azopolymers.

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Nanoengineered Surfaces for Focal Adhesion Guidance Trigger Mesenchymal Stem Cell Self-Organization and Tenogenesis

Cited by 49 publications

References 30 publications

Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics

Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics

Engineering Cell Instructive Materials To Control Cell Fate and Functions through Material Cues and Surface Patterning

Reversible Holographic Patterns on Azopolymers for Guiding Cell Adhesion and Orientation

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