Nanoengineered polymeric S-layers based capsules with targeting activity

Habibi, Neda; Pastorino, Laura; Soumetz, Federico Caneva; Sbrana, Francesca; Raiteri, Roberto; Ruggiero, Carmelina

doi:10.1016/j.colsurfb.2011.07.015

Cited by 37 publications

(27 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The important innovation in the LbL technique is represented by its use of the assembly of multilayered structures on the surface of micro/nanoparticles followed by their successive removal to obtain hollow nanoshells [13][14][15][16]. The obtained nanoshells have diameters ranging in size from tens of nanometers to tens of microns and thicknesses ranging from a few nanometers to hundreds of nanometers.…”

Section: Introductionmentioning

confidence: 99%

Permeability Variation Study in Collagen-Based Polymeric Capsules

et al. 2011

Self Cite

View full text Add to dashboard Cite

Nanomedicine, the application of nanotechnologies to healthcare, is an area of nanotechnology which is promising and has extraordinary and far-reaching implications on human health both from the points of view of disease diagnosis and treatment. With regard to disease treatment, the possibility of developing new nanosystems for efficient drug delivery has attracted great attention. A prerequisite for nanotechnology-based delivery systems, in order to be effective, is that the encapsulated drug molecules have to be released at the site of interest. One method to obtain specific release is based on the development of systems sensitive to disease-associated overexpressed enzymes. In this study, we report on the fabrication of collagen-based containers for drug encapsulation and release by cellular demand by the action of the collagenolytic enzymes matrix metalloproteinases (MMPs).Collagen is the major component of the connective tissue and is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of MMPs is the cause of such degradation. Collagen-based capsules were fabricated by the layer-by-layer technique and characterized by scanning electron microscopy and confocal microscopy. Real-time monitoring of the release kinetics of loaded gold nanoparticles, having different diameters, was done by total reflection X-ray fluorescence. Excitation of the fluorescence was performed by grazing incidence X-ray beam of synchrotron radiation. This study represents the first attempt in estimating the distribution and dimension of pore size in polyelectrolyte nanoshells.Keywords Collagen . Matrix metalloproteinase . Polyelectrolyte capsules . Enzyme-triggered release . Permeability variation BackgroundRecent advancements in nanotechnology and biotechnology have opened new scenarios in healthcare, both from the diagnostic, imaging, and therapeutic points of view [1].One of the most relevant areas of nanomedicine is represented by the development of nanosystems for the efficient delivery of pharmaceutically relevant molecules to a specific site with specific features [2]. Such nanosystems hold promise to overcome problems encountered with the use of traditional drug formulation and, thus, to develop new therapeutic strategies. Nanosystems can be potentially designed to deliver encapsulated drugs to the target site, to trigger the release of cargo molecules by external or internal stimuli, and to control drug pharmacokinetic profiles as required by specific therapeutic protocols. Different successful approaches for delivery to the site of pathology

show abstract

Section: Introductionmentioning

confidence: 99%

Permeability Variation Study in Collagen-Based Polymeric Capsules

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…This allowed the resolution of structural details of protein alignment and spacing [92,93]. In 2011, Habibi et al used AFM to imaging the controlled crystallization of IgG functionalized-SLPs from Bacillus thuringiensis onto the shell of hollow polyelectrolyte capsules for targeted drug delivery application [94].…”

Section: A Flower-shaped Surface Of the Native S-layer (Scale Bar = mentioning

confidence: 99%

“…They allow the investigation of the mechanisms of recrystallization of SLPs at a nanoscale level and therefore it is important for understanding and engineering of biomembranes and biosensors [110]. QCM has for instance been used to follow in situ the self-assembly of SLPs from solution to solid substrates on gold or SiO 2 surfaces [111] and also used to investigate in situ the electrochemical behavior of the SLPs on gold electrodes with the capability to measure mass changes at the nanogram level at the electrode surface/electrolyte interfaces [94,112,113].…”

Section: Quartz Crystal Microbalance and Surface Plasmon Resonancementioning

confidence: 99%

Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction

Mobili

2013

IJBCRR

View full text Add to dashboard Cite

Surface-layers (S-layers) are macromolecular paracrystalline arrays of proteins or glycoproteins that can self-assemble into 2-dimensional semi-permeable meshworks to overlay the cell surface of many bacteria and archaea. They usually assemble into lattices with oblique, square or hexagonal symmetry and serve as an interface between the bacterial cell and the environment. Isolated S-layers can recrystallize into two-dimensional regular arrays in suspension or on various surfaces, thus being an appropriate material for several bionanotechnological purposes. Promising applications of S-layers include their use as biotemplates for the capture of metal ions or the synthesis of metal nanoclusters. Research ArticleInternational Journal of Biochemistry Research & Review, 3(1): 39-62, 2013 40 Considering the use of S-layers as biotemplates for the organization of metal ions or metallic nanoclusters, research on potential of surface layer proteins (SLP) and metals can be understood as an interdisciplinary field, in which different biophysical techniques supply complementary information. In this review, we discuss the SLP as native or engineered "bottom-up" building blocks for metal immobilization structures. We also describe the biophysical techniques used to analyze metal binding properties as well as the information obtained from the investigation of these structures.

show abstract

“…Ultimately, systems biology lays the basis for engineering organisms, a discipline defined as synthetic biology. Synthetic biology encompasses novel approaches developed in chemistry, namely, artificial cells constructed with nanostructured polymeric capsules or nanoreactors (van Dongen et al, 2010;Habibi et al, 2011), as well as attempts to redesign networks and pathways (e.g., to produce products in plants as factories) from the wildest dreams of building organisms from scratch. As a step in this direction, we are now able to synthesize complete chromosomes (Gibson et al, 2010).…”

Section: Systems Biology and Synthetic Biologymentioning

confidence: 99%

New Technologies for 21st Century Plant Science

Ehrhardt

Frommer

2012

Plant Cell

View full text Add to dashboard Cite

Plants are one of the most fascinating and important groups of organisms living on Earth. They serve as the conduit of energy into the biosphere, provide food, and shape our environment. If we want to make headway in understanding how these essential organisms function and build the foundation for a more sustainable future, then we need to apply the most advanced technologies available to the study of plant life. In 2009, a committee of the National Academy highlighted the "understanding of plant growth" as one of the big challenges for society and part of a new era which they termed "new biology." The aim of this article is to identify how new technologies can and will transform plant science to address the challenges of new biology. We assess where we stand today regarding current technologies, with an emphasis on molecular and imaging technologies, and we try to address questions about where we may go in the future and whether we can get an idea of what is at and beyond the horizon.We believe that the major three challenges for humankind in the 21st century are food, energy, and the environment, including climate change and environmental degradation due to pollution and habitat loss. In a word: sustainability. Plant life plays an essential role in all three of these sustainability challenges. All of our food and the majority of our energy are produced by photosynthetic plants. Plants are major players in determining our climate, and agricultural expansion is a major factor in habitat encroachment and pollution of waterways by fertilizer application and runoff. Furthermore, these issues are not independent; as the climate changes, additional challenges are placed on plant performance and, thus, food supply and habitat. Research on plants is needed to provide solutions to these major challenges.The fundamental biology of plants is similar to our own; they use the same genetic code, share many homologous genes, and even many regulatory mechanisms, basic biochemical pathways, and fundamental processes in cell biology. However, their form and lifestyle are fundamentally different. Plants can reach individual life spans of up to 5000 years; they can obtain adequate nutrition from the air and soil and survive adverse environmental conditions and attacks from pests, pathogens, and herbivores, despite remaining rooted in one spot for their lifetime. Plants are master chemists and can defend themselves with an incredible arsenal of chemicals. Many plants do not have a determinate body plan; a single genome is capable of producing an enormous range of size and form. Thus, plants are also valuable basic research objects since we can learn fundamental principles that are shared with humans and at the same time learn how different wiring can create such fundamental differences in form, biochemistry, and function.While some of the richest ideas in the life sciences have been developed without application of specialized technology (the theory of natural selection and evolution stands out as a prime example), technology is mo...

show abstract

Nanoengineered polymeric S-layers based capsules with targeting activity

Cited by 37 publications

References 46 publications

Permeability Variation Study in Collagen-Based Polymeric Capsules

Permeability Variation Study in Collagen-Based Polymeric Capsules

Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction

New Technologies for 21st Century Plant Science

Contact Info

Product

Resources

About