Nanoencapsulation of ultra-small superparamagnetic particles of iron oxide into human serum albumin nanoparticles

Abstract

SummaryHuman serum albumin nanoparticles have been utilized as drug delivery systems for a variety of medical applications. Since ultra-small superparamagnetic particles of iron oxide (USPIO) are used as contrast agents in magnetic resonance imaging, their encapsulation into the protein matrix enables the synthesis of diagnostic and theranostic agents by surface modification and co-encapsulation of active pharmaceutical ingredients. The present investigation deals with the surface modification and nanoencapsulation of USPIO into an albumin matrix by using ethanolic desolvation. Particles of narrow size distribution and with a defined particle structure have been achieved.

Protein nanoparticles with ligand-binding and enzymatic activities

“…With modifications, some of the previously developed approaches including alcohol or acetone desolvation, emulsification, spray drying and thermal denaturation can be applied for a number of proteins. 2,9,11 To produce protein NP ethanol, 12 acetone 13 and polyvalent ions 14 can be used as anti-solvents or desolvation/precipitation agents. 15 Unfortunately, most currently known methods of protein NP construction can be applied to certain proteins only.…”

Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy

“…Mannitol, sucrose, and trehalose were evaluated as lyoprotectants at a concentration of 3% (w/v). For this purpose, a volume of 1.0 mL containing 10 mg/mL of targeted theranostic particles and a lyoprotectant was freeze-dried in a Christ Epsilon 2-7 freeze dryer (Martin Christ GmbH, Osterrode, Germany) as described previously [22,30]. The physical stability of the targeted theranostic nanocarriers was investigated after 6 months of storage at 4 °C and reconstitution in the same volume of MilliQ water.…”

“…To encapsulate USPIO into albumin nanocarriers, the desolvation method was applied [35]. Presumably both, a positive surface charge and Ce3/4 + cations coordinative linkage led to a strong interaction with the negatively charged matrix material [22].…”

“…Exploring the biodistribution of nanocarriers is an important requirement for clinical translation of nanocarrier delivery. In this context, ultra-small superparamagnetic particles of iron oxide (USPIO) were encapsulated into HSA nanoparticles serving as contrast agents for magnetic resonance (MR) imaging [21,22]. A novel species of First revision maghemite core particles was used [23][24][25].…”