Nanoencapsulation of bovine lactoferrin for food and biopharmaceutical applications

Abstract

Lactoferrin has for long captured the interest of many researchers as a natural compound with a wide variety of uses. Lactoferrin is a monomeric, iron-binding 80 kDa glycoprotein, and appears to be the subfraction of whey with the best documented antiviral, antimicrobial, anticancer and immune modulating/enhancing effects. It belongs to the family of transferrin proteins, and serves to control iron levels in body fluids by sequestering and solubilizing ferric iron. In the present research effort, production of lactoferrin derivatives (starting from a purified commercial extract), encompassing full stabilization of its three-dimensional structure, has been attempted via nanoencapsulation within lipid nanovesicles, integrating a multiple water-in-oil-in-water emulsion. Long-term storage of the multiple nanoemulsions produced did not lead to leaching of protein, thus proving the effectiveness of the encapsulation procedure. Furthermore, lactoferrin nanovesicle derivatives prepared under optimal conditions were successfully employed at lab-scale antimicrobial trials.

Biomimetic aqueous-core lipid nanoballoons integrating a multiple emulsion formulation: A suitable housing system for viable lytic bacteriophages

“…Additionally, imprisonment of a protein entity (such as the bacteriophage particles entertained in the present research effort) promotes a change in the thermodynamic conditions of the nanoenvironment surrounding each biomolecule due to the fact that the movements of (aqueous) solvent molecules in their microneighborhood become seriously reduced by the effect of being contained within the matrix's aqueous core, thereby leading to enhanced thermodynamic stability. The net result is an enhancement of the protein entity's rotational, translational and vibrational viscosity, promoting a more rigid three-dimensional architecture of the biomolecule with concomitant decrease of entropy and thus promoting its structural and functional stabilization [3][4][5][6]. The use of these multiple emulsion systems, provided the correct phage particles are used, e.g.…”

“…When compared to antibiotics, phages present many relevant advantages: (i) permanently high concentrations at the infection site, increasing in the presence of (viable) bacterial host cells, with elimination occurring only after eradication of the later; (ii) total compatibility with antibiotics; (iii) high specificity against target-bacteria; (iv) higher penetration into bacterial biofilms, by inducing production of enzymes that hydrolyze biofilm polymeric matrix; and (v) while bacteria can develop resistance to phages, isolation and large-scale production of new lytic phages is much simpler and economical than developing a new antibiotic [17,34,35]. Water-in-oil-in-water (W/O/W) emulsions are examples of multiple emulsions, in which dispersions of small water droplets within larger oil droplets are themselves dispersed in a continuous aqueous phase [5,7,11,[14][15][16]36]. Due to their compartimentalized internal structure, multiple emulsions present advantages over simple oil-in-water (O/W) emulsions for encapsulation, such as the ability to carry both polar and non-polar molecules, and a better control over releasing of therapeutic molecules [10,19,21,23].…”

Functionalized Polymers for Enhance Oral Bioavailability of Sensitive Molecules

“…Bovine Lactoferrin stabilized with poloxamers (PEO-PPO-PEO) Lactoferrin [52] In vitro, Protective degradation of drug and antimicrobial trials.…”

Estudo Do Efeito Das Coordenadas Termodinâmicas Sobre O Escoamento No Interior De Uma Câmara De Precipitação De Partículas

“…Técnicas para precipitação de micro e nanopartículas empregando fluido em estado supercrítico têm sido modificadas e exploradas para aplicações nas mais diversas áreas incluindo a indústria farmacêutica, cosmética, alimentícia e outras como alternativas aos processos tradicionais (Balcão, et al, 2013; E.M. Berends, 1993;Martín, et al, 2007).…”