Nanoencapsulation Improves the &lt;I&gt;In Vitro&lt;/I&gt; Antioxidant Activity of Lipoic Acid

Külkamp, Irene Clemes; Rabelo, Bruna D; Berlitz, Simone Jacobus; Isoppo, Mateus; Bianchin, Mariana Domingues; Schaffazick, Scheila Rezende; Pohlmann, Adriana Raffin; Guterres, Sílvia Stanisçuaski

doi:10.1166/jbn.2011.1318

Cited by 41 publications

(26 citation statements)

References 26 publications

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“…Lipoic acid is a supplement with antioxidant properties that can be measured in the laboratory by its inhibitory effects on lipid peroxidation (Kulkamp et al, 2011). PCL nanocapsules have been shown to increase the antioxidant activity of lipoic acid as compared to the activity of lipoic acid in a control solution (Kulkamp et al, 2011).…”

Section: Polymers (Soluble/insoluble Nanomaterials)mentioning

confidence: 99%

“…PCL nanocapsules have been shown to increase the antioxidant activity of lipoic acid as compared to the activity of lipoic acid in a control solution (Kulkamp et al, 2011). The beneficial effect of PCL encapsulation is thought to be due to stabilization of the lipoic acid in combination with a slow, prolonged release of the compound.…”

Section: Polymers (Soluble/insoluble Nanomaterials)mentioning

confidence: 99%

See 1 more Smart Citation

Nanotechnology in cosmetics

Katz

Dewan

Bronaugh

2015

Food and Chemical Toxicology

144

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Section: Polymers (Soluble/insoluble Nanomaterials)mentioning

confidence: 99%

Section: Polymers (Soluble/insoluble Nanomaterials)mentioning

confidence: 99%

Nanotechnology in cosmetics

Katz

Dewan

Bronaugh

2015

Food and Chemical Toxicology

144

View full text Add to dashboard Cite

“…[61][62][63] It is beyond question that involvement of reactive oxygen species (ROS) and RNS affects melanoma induction and development, but the complexity of actions has generated conflicting data. [64][65][66] Therefore, we will make efforts to characterize the mechanisms of LNC on ROS and RNS systems and to establish the real role of ROS/RNS balance and its involvement in the toxicity of LNC on melanoma cells.…”

Section: Drewes Et Almentioning

confidence: 99%

Novel therapeutic mechanisms determine the effectiveness of lipid-core nanocapsules on melanoma models

Drewes¹,

Fiel²,

Bexiga³

et al. 2016

IJN

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Melanoma is a severe metastatic skin cancer with poor prognosis and no effective treatment. Therefore, novel therapeutic approaches using nanotechnology have been proposed to improve therapeutic effectiveness. Lipid-core nanocapsules (LNCs), prepared with poly(ε-caprolactone), capric/caprylic triglyceride, and sorbitan monostearate and stabilized by polysorbate 80, are efficient as drug delivery systems. Here, we investigated the effects of acetyleugenol-loaded LNC (AcE-LNC) on human SK-Mel-28 melanoma cells and its therapeutic efficacies on melanoma induced by B16F10 in C57B6 mice. LNC and AcE-LNC had z -average diameters and zeta potential close to 210 nm and -10.0 mV, respectively. CytoViva ® microscopy images showed that LNC and AcE-LNC penetrated into SK-Mel-28 cells, and remained in the cytoplasm. AcE-LNC in vitro treatment (18–90×10 9 particles/mL; 1 hour) induced late apoptosis and necrosis; LNC and AcE-LNC (3–18×10 9 particles/mL; 48 hours) treatments reduced cell proliferation and delayed the cell cycle. Elevated levels of nitric oxide were found in supernatant of LNC and AcE-LNC, which were not dependent on nitric oxide synthase expressions. Daily intraperitoneal or oral treatment (days 3–10 after tumor injection) with LNC or AcE-LNC (1×10 12 particles/day), but not with AcE (50 mg/kg/day, same dose as AcE-LNC), reduced the volume of the tumor; nevertheless, intraperitoneal treatment caused toxicity. Oral LNC treatment was more efficient than AcE-LNC treatment. Moreover, oral treatment with nonencapsulated capric/caprylic triglyceride did not inhibit tumor development, implying that nanocapsule supramolecular structure is important to the therapeutic effects. Together, data herein presented highlight the relevance of the supramolecular structure of LNCs to toxicity on SK-Mel-28 cells and to the therapeutic efficacy on melanoma development in mice, conferring novel therapeutic mechanisms to LNC further than a drug delivery system.

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“…The rose-hip oil oxidation under UVA and UVC lights was studied by means of the lipoperoxidation process, quantifying the fluorescent product obtained by the reaction of malondialdehyde and thiobarbituric acid at 532 nm by UV spectroscopy (27). The malondialdehyde is one of the most frequently used indicators of lipid peroxidation.…”

Section: In Vitro Oxidation Studymentioning

confidence: 99%

Nanoencapsulation of Rose-Hip Oil Prevents Oil Oxidation and Allows Obtainment of Gel and Film Topical Formulations

et al. 2015

Self Cite

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Abstract. The rose-hip oil holds skin regenerating properties with applications in the dermatological and cosmetic area. Its nanoencapsulation might favor the oil stability and its incorporation into hydrophilic formulations, besides increasing the contact with the skin and prolonging its effect. The aim of the present investigation was to develop suitable rose-hip-oil-loaded nanocapsules, to verify the nanocapsule effect on the UV-induced oxidation of the oil and to obtain topical formulations by the incorporation of the nanocapsules into chitosan gel and film. The rose-hip oil (500 or 600 μL), polymer (Eudragit RS100®, 100 or 200 mg), and acetone (50 or 100 mL) contents were separately varied aiming to obtain an adequate size distribution. The results led to a combination of the factors acetone and oil. The developed formulation showed average diameter of 158±6 nm with low polydispersity, pH of 5.8±0.9, zeta potential of +9.8 ±1.5 mV, rose-hip oil content of 54±1 μL/mL and tendency to reversible creaming. No differences were observed in the nanocapsules properties after storage. The nanoencapsulation of rose-hip oil decreased the UVA and UVC oxidation of the oil. The chitosan gel and film containing rose-hip-oil-loaded nanocapsules showed suitable properties for cutaneous use. In conclusion, it was possible to successfully obtain rose-hip-oil-loaded nanocapsules and to confirm the nanocapsules effect in protecting the oil from the UV rays. The chitosan gel and film were considered interesting alternatives for incorporating the nanoencapsulated rose-hip oil, combining the advantages of the nanoparticles to the advantages of chitosan.

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Nanoencapsulation Improves the <I>In Vitro</I> Antioxidant Activity of Lipoic Acid

Cited by 41 publications

References 26 publications

Nanotechnology in cosmetics

Nanotechnology in cosmetics

Novel therapeutic mechanisms determine the effectiveness of lipid-core nanocapsules on melanoma models

Nanoencapsulation of Rose-Hip Oil Prevents Oil Oxidation and Allows Obtainment of Gel and Film Topical Formulations

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