Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers

Kaiser, M.; Pohl, Luisa; Ketelhut, Steffi; Kastl, Lena; Gorzelanny, Christian; Götte, Martin; Schnekenburger, Jürgen; Goycoolea, Francisco M.; Kemper, Björn

doi:10.1371/journal.pone.0187497

Cited by 16 publications

(10 citation statements)

References 67 publications

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“…As cofilin was able to depolymerize actin structures, we think that its activation was responsible for the observed reorganization of the actin skeleton and the connected tight junction proteins. Similar results were obtained with primary mouse endothelial cells and in our previous studies with MDCK cells [18]. The staining of other tight junction proteins in future studies could be extremely informative towards the elucidation of the mechanistic underpinning at play.…”

Section: Discussionsupporting

confidence: 85%

The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers

Kaiser

Burek

Britz

et al. 2018

IJMS

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Microvascular endothelial cells are an essential part of many biological barriers, such as the blood–brain barrier (BBB) and the endothelium of the arteries and veins. A reversible opening strategy to increase the permeability of drugs across the BBB could lead to improved therapies due to enhanced drug bioavailability. Vanilloids, such as capsaicin, are known to reversibly open tight junctions of epithelial and endothelial cells. In this study, we used several in vitro assays with the murine endothelial capillary brain cells (line cEND) as a BBB model to characterize the interaction between capsaicin and endothelial tight junctions.

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Section: Discussionsupporting

confidence: 85%

The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers

Kaiser

Burek

Britz

et al. 2018

IJMS

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“…Because capsaicin can also promote cell migration 53 , 54 , the dynamic behaviour of the cells was analysed by calculating frame-to-frame differences in our videos. In comparison to untreated cells (control), cellular motilities of T24 and UROtsa cells were slightly higher for few minutes after the administration (time point 0 h) of Chi-NCs and Chi-NC-Cap (5.6 × 10 12 particles mL −1 = 500 µm capsaicin).…”

Section: Resultsmentioning

confidence: 99%

Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules

Palubitzki

Wang

Hoffmann

et al. 2020

Sci Rep

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The glycocalyx regulates the interaction of mammalian cells with extracellular molecules, such as cytokines. However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding and uptake of nanoparticles. In the present study, exome sequencing data of cancer patients and analysis of distinct melanoma and bladder cancer cell lines suggested differences in cancer cell-exposed glycocalyx components such as heparan sulphate. Our data indicate that glycocalyx differences affected the binding of cationic chitosan nanocapsules (Chi-NCs). The pronounced glycocalyx of bladder cancer cells enhanced the internalisation of nanoencapsulated capsaicin. Consequently, capsaicin induced apoptosis in the cancer cells, but not in the less glycosylated benign urothelial cells. Moreover, we measured counterion condensation on highly negatively charged heparan sulphate chains. Counterion condensation triggered a cooperative binding of Chi-NCs, characterised by a weak binding rate at low Chi-NC doses and a strongly increased binding rate at high Chi-NC concentrations. Our results indicate that the glycocalyx of tumour cells controls the binding and biological activity of nanoparticles. This has to be considered for the design of tumour cell directed nanocarriers to improve the delivery of cytotoxic drugs. Differential nanoparticle binding may also be useful to discriminate tumour cells from healthy cells.

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“…Particle-induced cytotoxicity can also be determined by other label-free methods using confluent cell layers, such as measurement of transepithelial electrical resistance (TEER) [58], with limitations compared to DHM concerning costs, maximum sample numbers and sensitivity to detect morphology changes, or histogram-based evaluation of quantitative DHM phase contrast images [59]. An advantage of these methods for the toxicity assessment is that they do not rely on cell growth rate or dry mass increment, which can depend on specific cell culture conditions but only partly reflect the characteristics of proliferating cells.…”

Section: Discussionmentioning

confidence: 99%

Interlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers

Marzi¹,

Eder²,

Barroso³

et al. 2022

Drug Deliv. and Transl. Res.

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State-of-the-art in vitro test systems for nanomaterial toxicity assessment are based on dyes and several staining steps which can be affected by nanomaterial interference. Digital holographic microscopy (DHM), an interferometry-based variant of quantitative phase imaging (QPI), facilitates reliable proliferation quantification of native cell populations and the extraction of morphological features in a fast and label- and interference-free manner by biophysical parameters. DHM therefore has been identified as versatile tool for cytotoxicity testing in biomedical nanotechnology. In a comparative study performed at two collaborating laboratories, we investigated the interlaboratory variability and performance of DHM in nanomaterial toxicity testing, utilizing complementary standard operating procedures (SOPs). Two identical custom-built off-axis DHM systems, developed for usage in biomedical laboratories, equipped with stage-top incubation chambers were applied at different locations in Europe. Temporal dry mass development, 12-h dry mass increments and morphology changes of A549 human lung epithelial cell populations upon incubation with two variants of poly(alkyl cyanoacrylate) (PACA) nanoparticles were observed in comparison to digitonin and cell culture medium controls. Digitonin as cytotoxicity control, as well as empty and cabazitaxel-loaded PACA nanocarriers, similarly impacted 12-h dry mass development and increments as well as morphology of A549 cells at both participating laboratories. The obtained DHM data reflected the cytotoxic potential of the tested nanomaterials and are in agreement with corresponding literature on biophysical and chemical assays. Our results confirm DHM as label-free cytotoxicity assay for polymeric nanocarriers as well as the repeatability and reproducibility of the technology. In summary, the evaluated DHM assay could be efficiently implemented at different locations and facilitates interlaboratory in vitro toxicity testing of nanoparticles with prospects for application in regulatory science. Graphical abstract

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Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers

Cited by 16 publications

References 67 publications

The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers

The Influence of Capsaicin on the Integrity of Microvascular Endothelial Cell Monolayers

Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules

Interlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers

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