Nanoemulsions to support ex vivo cell culture of breast cancer circulating tumor cells

Carmona-Ule, Nuria; Abuín-Redondo, C.; Costa, Clotilde; Piñeiro, Roberto; Pereira-Veiga, Thais; Martínez-Pena, Inés; Hurtado, Pablo; López‐López, Rafael; Fuente, María de la; Dávila-Ibáñez, Ana B.

doi:10.1016/j.mtchem.2020.100265

Cited by 5 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous work showed that after exogenous addition of oleic acid to breast cancer samples, CD36 expression increased, followed by accumulation of cytoplasmic lipid droplets only in CD36-expressing cells [84]. These findings are in agreement with our previous work where we observed an accumulation of cytoplasmic lipid droplets in breast cancer cells after the addition of NEs, which lead to increased cell viability [45]. In addition, CD36 was already linked with metastatic potential in breast and melanoma cancer metastasis [85] and as a key element of survival in resistant breast cancer cells [86].…”

Section: Discussionsupporting

confidence: 93%

“…For that, we choose a negative isolation CTCs enrichment approach, to guarantee minimal cell damage and to obtain a heterogeneous CTC population. The CTCs were cultured under ultra-low attachment conditions using a specific protocol, which includes the addition of NEs as previously described by our group [45]. The addition of biocompatible NEs composed of lipid and fatty acids to support CTC cultures, improving their proliferation capacity, has not been reported to date.…”

Section: Discussionmentioning

confidence: 99%

“…Cell cultures were supplemented with fresh medium every 2 days with minimal well disturbance to avoid cell loss. NEs were prepared as previously described [45], and were added to the media every two days at the same time as the fresh medium supplement. Before addition to the media, NEs were filtered using Acrodisc® PFS Syringe filters and mix with cell medium (concentration ranges were within 6.7x10 8 particle/mL to 2.14x10 9 particle/mL).…”

Section: Ctc Isolation and Ex Vivo Culturementioning

confidence: 99%

“…In this regard, we have recently published a protocol that uses Nanoemulsions (NEs) formulated from lipids and fatty acid to promote the proliferation of breast cancer cell lines and CTCs from a mouse model derived from breast cancer cell line [45]. Our study was based on previous studies that reported how mitochondrial metabolism (including oxidation of glutamine and fatty acids) or the accumulation of lipids and free fatty acids is linked to invasion, aggressiveness, migration, and progression of breast cancer [46][47][48].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Short-Term Ex Vivo Culture of CTCs From Advance Breast Cancer Patients: Clinical Implications

Carmona-Ule

González-Conde

Abuín

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Circulating tumor cells (CTC) have relevance as prognostic markers in breast cancer. However, the functional properties of CTCs or their molecular characterization have not been well-studied. Experimental models indicate that only a few cells can survive in the circulation and eventually metastasize. Thus, it is essential to identify these surviving cells capable of forming such metastases. Methods: We isolated viable CTCs from 50 peripheral blood samples obtained from 35 patients with advanced metastatic breast cancer using RosetteSepTM for ex vivo culture. The CTCs were seeded and monitored on plates under low adherence conditions and with media supplemented with growth factors and Nanoemulsions. Phenotypic analysis was performed by immunofluorescence and gene expression analysis using RT-PCR and CTCs counting by Cellsearch® system. Results: We found that in 75% of samples the CTC cultures lasted more than 23 days, predicting a shorter Progression-Free Survival in these patients, independently of having ≥ 5 CTC by Cellsearch®. We also observed that CTCs before and after culture showed a different gene expression profile. Conclusions: the cultivability of CTCs is a predictive factor. Furthermore, the subset of cells capable of growing ex vivo show stem or mesenchymal features and may represent the CTC population with metastatic potential in vivo.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Ctc Isolation and Ex Vivo Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Short-Term Ex Vivo Culture of CTCs From Advance Breast Cancer Patients: Clinical Implications

Carmona-Ule

González-Conde

Abuín

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In order to improve the drawbacks related to the limitations of CTC isolation technologies, we developed a bottom-up methodology to formulate Peptide-Functionalized Nanoemulsions (Pept-NEs) targeting cancer cells. Our NEs have been previously shown to favor the proliferation of breast cancer cells and CTCs isolated from patients in culture [ 25 , 26 ]. Hence, the aim of this work was to develop a formulation which is able to isolate CTCs and culture them in a single step through the use of a tailored microfluidic device and exploiting the favorable effect observed on CTC cultures when supported with NEs.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Functionalized Nanoemulsions as a Promising Tool for Isolation and Ex Vivo Culture of Circulating Tumor Cells

et al. 2022

View full text Add to dashboard Cite

Circulating Tumor Cells (CTCs) are shed from primary tumors and travel through the blood, generating metastases. CTCs represents a useful tool to understand the biology of metastasis in cancer disease. However, there is a lack of standardized protocols to isolate and culture them. In our previous work, we presented oil-in-water nanoemulsions (NEs) composed of lipids and fatty acids, which showed a benefit in supporting CTC cultures from metastatic breast cancer patients. Here, we present Peptide-Functionalized Nanoemulsions (Pept-NEs), with the aim of using them as a tool for CTC isolation and culture in situ. Therefore, NEs from our previous work were surface-decorated with the peptides Pep10 and GE11, which act as ligands towards the specific cell membrane proteins EpCAM and EGFR, respectively. We selected the best surface to deposit a layer of these Pept-NEs through a Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) method. Next, we validated the specific recognition of Pept-NEs for their protein targets EpCAM and EGFR by QCM-D and fluorescence microscopy. Finally, a layer of Pept-NEs was deposited in a culture well-plate, and cells were cultured on for 9 days in order to confirm the feasibility of the Pept-NEs as a cell growth support. This work presents peptide-functionalized nanoemulsions as a basis for the development of devices for the isolation and culture of CTCs in situ due to their ability to specifically interact with membrane proteins expressed in CTCs, and because cells are capable of growing on top of them.

show abstract

Emerging tumor-on-chips with electrochemical biosensors

Lei

et al. 2022

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

Nanoemulsions to support ex vivo cell culture of breast cancer circulating tumor cells

Cited by 5 publications

References 53 publications

Short-Term Ex Vivo Culture of CTCs From Advance Breast Cancer Patients: Clinical Implications

Short-Term Ex Vivo Culture of CTCs From Advance Breast Cancer Patients: Clinical Implications

Peptide-Functionalized Nanoemulsions as a Promising Tool for Isolation and Ex Vivo Culture of Circulating Tumor Cells

Emerging tumor-on-chips with electrochemical biosensors

Contact Info

Product

Resources

About