Nanoemulsion: A suitable nanodelivery system of clove oil for anesthetizing Nile tilapia

Kheawfu, Kantaporn; Pikulkaew, Surachai; Chaisri, Wasana; Okonogi, Siriporn

doi:10.5582/ddt.2017.01029

Cited by 15 publications

(3 citation statements)

References 21 publications

(19 reference statements)

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“…These compounds have been linked to several pharmacological actions [16][17][18][46][47][48][49][50]55,57]. Other authors reported similar results [12,47,[64][65][66][67][68][69][70]. These findings are reported in this study.…”

Section: Discussionsupporting

confidence: 88%

Insights from Syzygium aromaticum Essential Oil: Encapsulation, Characterization, and Antioxidant Activity

Mergulhão,

Bulhões,

Silva

et al. 2024

Pharmaceuticals

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Alginate encapsulates loaded with clove essential oil (CEO) were prepared by ionic gelation, with subsequent freeze-drying. The objective of the present work was to develop a product with the ability to protect CEO against its easy volatility and oxidation. The following techniques were used to characterize the formulations: eugenol release, degree of swelling, GC/MS, TGA/DSC, and SEM. The alginate solution (1.0%) containing different concentrations of CEO (LF1: 1.0%; LF2: 0.5%; LF3: 0.1%) was dropped into a 3.0% CaCl2 solution. After lyophilization, the encapsulated samples were wrinkled and rigid, with high encapsulation power (LF3: 76.9% ± 0.5). Three chemical components were identified: eugenol (the major one), caryophyllene, and humulene. The antioxidant power (LF1: DPPH IC50 18.1 µg mL−1) was consistent with the phenol content (LF1: 172.2 mg GAE g−1). The encapsulated ones were thermally stable, as shown by analysis of FTIR peaks, eugenol molecular structure was kept unaltered. The degree of swelling was 19.2% (PBS). The release of eugenol (92.5%) in the PBS solution was faster than in the acidic medium. It was concluded that the low-cost technology used allows the maintenance of the content and characteristics of CEO in the three concentrations tested, offering a basis for further research with essential oil encapsulates.

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Section: Discussionsupporting

confidence: 88%

Insights from Syzygium aromaticum Essential Oil: Encapsulation, Characterization, and Antioxidant Activity

Mergulhão,

Bulhões,

Silva

et al. 2024

Pharmaceuticals

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“…CO was purchased from Thai-China Flavors and Fragrances Industry (Nonthaburi, Thailand). The obtained CO was analyzed using gas chromatography–mass spectrometry (GC-MS) and the method previously described [ 23 ]. Nile Red, polysorbate 20, Kolliphor EL, methanol, acetonitrile, GABA, diazepam, eugenol, and dimethyl sulfoxide (DMSO) were from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish

et al. 2022

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Clove oil (CO), an essential oil of Syzygium aromaticum, has been reported as an anesthetic for many fish species. However, its insoluble properties require a suitable delivery system for its application. In the present study, nanoformulations of CO as a nanoemulsion (CO-NE), a self-microemulsifying drug-delivery system (CO-SMEDDS), and a self-nanoemulsifying drug-delivery system (CO-SNEDDS) were prepared for delivering CO. Zebrafish were used as a fish model to investigate oil pathways. The result shows fluorescence spots of fluorescence-labeled CO accumulate on the gills, skin, and brain. All CO nanoformulations significantly increased penetration flux compared to CO ethanolic solution. Investigation of the anesthetic mechanism of action using a rat brain γ-aminobutyric acid subtype A (GABAA) receptor-binding test demonstrates that CO and its major compound, eugenol, modulate [3H]muscimol binding. CO-NE exhibited a concentration-dependent binding activity with an EC50 value of 175 µg/mL, significantly higher than CO solution in dimethyl sulfoxide. In conclusion, CO enters the fish through the skin and gills. The anesthetic mechanism of action of CO is based on modulation of [3H] muscimol binding to GABAA receptors. Among three nanoformulations tested, CO-NE is the most effective at increasing permeability and enhancing the receptor-binding activity of the oil.

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“…NE containing 10% CAEO (NE-CA) was prepared according to the method previously described [ 28 ] with some modification and using 25% Tween 80 as a surfactant. The mixture composed of CAEO, surfactant, and water was subjected to Ultra-Turrax T25 (Janke and Kunkel GmbH, Staufen, Germany) at a high-speed stirring rate of 12,000 rpm for 30 s to obtain a pre-emulsion.…”

Section: Methodsmentioning

confidence: 99%

Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects

Panyajai,

Viriyaadhammaa,

Tima

et al. 2024

BMC Complement Med Ther

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Background and aims Curcuma aeruginosa, commonly known as “kha-min-dam” in Thai, holds significance in Asian traditional medicine due to its potential in treating various diseases, having properties such as anti-HIV, hepatoprotective, antimicrobial and anti-androgenic activities. This study explores the anticancer activity of C. aeruginosa essential oil (CAEO) and its nano-formulations. Methods CAEO obtained from hydrodistillation of C. aeruginosa fresh rhizomes was examined by gas chromatography mass spectroscopy. Cytotoxicity of CAEO was determined in leukaemic K562 and breast cancer MCF-7 cell lines using an MTT assay. Cell cycle analysis and cell apoptosis were determined by flow cytometry. Cell migration was studied through a wound-healing assay. Results Benzofuran (33.20%) emerged as the major compound of CAEO, followed by Germacrene B (19.12%) and Germacrone (13.60%). Two types of CAEO loaded nano-formulations, nanoemulsion (NE) and microemulsion (ME) were developed. The average droplet sizes of NE and ME were 13.8 ± 0.2 and 21.2 ± 0.2 nm, respectively. In a comparison with other essential oils from the fresh rhizomes of potential plants from the same family (Curcuma longa, Curcuma mangga and Zingiber officinale) on anticancer activity against K562 and MCF-7 cell lines, CAEO exhibited the highest cytotoxicity with IC50 of 13.43 ± 1.09 and 20.18 ± 1.20 µg/mL, respectively. Flow cytometry analysis revealed that CAEO significantly increased cell death, evidenced from the sub-G1 populations in the cell cycle assay and triggered apoptosis. Additionally, CAEO effectively inhibited cell migration in MCF-7 cells after incubation for 12 and 24 h. The developed NE and ME formulations significantly enhanced the cytotoxicity of CAEO against K562 cells with an IC50 of 45.30 ± 1.49 and 41.98 ± 0.96 µg/mL, respectively. Conclusion This study’s finding suggest that both nano-formulations, NE and ME, effectively facilitated the delivery of CAEO into cancer cells.

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Nanoemulsion: A suitable nanodelivery system of clove oil for anesthetizing Nile tilapia

Cited by 15 publications

References 21 publications

Insights from Syzygium aromaticum Essential Oil: Encapsulation, Characterization, and Antioxidant Activity

Insights from Syzygium aromaticum Essential Oil: Encapsulation, Characterization, and Antioxidant Activity

Elucidating Pathway and Anesthetic Mechanism of Action of Clove Oil Nanoformulations in Fish

Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects

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