Nanoelectrokinetic Selective Preconcentration Based on Ion Concentration Polarization

Choi, Jihye; Baek, Seong-Ho; Kim, Hee Chan; Chae, Jong‐Hee; Koh, Youngil; Seo, Sang Woo; Lee, Hyomin; Kim, Sung Jae

doi:10.1007/s13206-020-4109-3

Cited by 20 publications

(14 citation statements)

References 88 publications

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“…In this context, developing new solutions for sample analyte preconcentration in bioanalytical fluidic devices remains a necessity. To address this prevalent issue, many focusing techniques based on electrokinetic phenomena have been proposed, among which ion concentration polarization [8‐20], field‐amplified sample stacking (FASS) [21‐23], concentration gradient focusing [24‐27], and isoelectric focusing [28‐30]. All these techniques allow focusing analytes by exploiting the unbalanced ionic transport between anionic and cationic species due to the competition between electroosmotic flow (EOF) and electrophoretic flow (EP).…”

Section: Introductionmentioning

confidence: 99%

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

et al. 2020

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Concentration polarization (CP)-based focusing electrokinetics nanofluidic devices have been developed in order to simultaneously detect and enrich highly diluted analytes ona-chip. However, stabilization of focal points over long time under the application of the electric field remains as a technical bottleneck. If pressure-assisted preconcentration methods have been proposed to stabilize propagating modes at low inverse Dukhin number (1/D u 1), these recent protocols remain laborious for optimizing experimental parameters. In this paper, "electric field E/counter-pressure P" diagrams have been established during pressure-assisted electro-preconcentration of fluorescein as a model molecule. Such E/P diagram allows direct observation of the region for which the optimal counterpressure P leads to a stable focusing regime. This region of stable focusing is shown to vary depending of the nanoslit length (100 μm < L nanoslit < 500 μm) and the nature of the background electrolyte (KCl and NaCl). Longer nanoslits (500 μm) produce stabilization at low counter-pressure P, whereas NaCl offers a narrower region of stable focusing in the E/P diagram compared to KCl. Finally, the ability of such pressure-assisted protocol to concentrate negatively charged proteins has been tested with a more applicative protein, i.e., ovalbumin. The corresponding E/P diagram confirms the existence of the stable focusing regime at both low electric field E (≤20 V) and counter-pressure P (≤0.4 bar). With an enrichment factor as high as 70 after 2 min for ovalbumin at a concentration of 10 μM, such pressure-assisted nanofluidic electro-preconcentration protocol appears very promising to concentrate and detect biomolecules.

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Section: Introductionmentioning

confidence: 99%

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

et al. 2020

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“…Micro-and nanofluidic devices based on ion concentration polarization (ICP) have been widely developed in recent decades, particularly in the context of protein and DNA separation and detection, analyte preconcentration, and the study of enzymatic reaction kinetics [1][2][3][4]. In such nanofluidic de-vices, separation, concentration, and detection of analytes can be obtained simultaneously under electric field stimuli using ICP-based focusing techniques.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to numerical studies, many microfluidicbased methods have been used for preconcentration of analytes on-chip such as ICP focusing [18][19][20] and periodic ICP [21]. A common fluidic architecture encountered in ICP-based preconcentration devices is one with a continuous microchannel and orthogonally oriented nanochannels branching away from the microchannel, wherein one applied field drives transport through the microchannel and another induces ICP across the nanochannels to form an extended depletion zone in the continuous microchannel [1,2,4,11,15,16,19,22]. While this architecture is more tunable and can achieve higher (∼10 6 -10 7 ) preconcentration factors than straight channels connecting two reservoirs, its fabrication and operation are more complex, and its somewhat specialized design is less common to nonpreconcentrationbased nanofluidic applications where ICP still plays a role.…”

Section: Introductionmentioning

confidence: 99%

Predicting ion concentration polarization and analyte stacking/focusing at nanofluidic interfaces

et al. 2022

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We report on the investigation of electropreconcentration phenomena in micro/nanofluidic devices integrating 100-µm-long nanochannels using 2D COMSOL simulations based on the coupled Poisson-Nernst-Planck and Navier-Stokes system of equations. Our numerical model is used to demonstrate the influence of key governing parameters such as electrolyte concentration, surface charge density, and applied axial electric field on ion concentration polarization (ICP) dynamics in our system. Under sufficiently extreme surfacecharge-governed transport conditions, ICP propagation is shown to enable various transient and stationary stacking and counter-flow gradient focusing mechanisms of anionic analytes. We resolve these spatiotemporal dynamics of analyte and electrolyte ICP over disparate time and length scales, and confirm previous findings that the greatest enhancement is observed when a system is tuned for analyte focusing at the charge-excluding microchannel-nanochannel electrical double layer (EDL) interface. Moreover, we demonstrate that such tuning can readily be achieved by including additional nanochannels oriented parallel to the electric field between two microchannels, effectively increasing the overall perm-selectivity and leading to enhanced focusing at the EDL interfaces. This approach shows promise in providing added control over the extent of ICP in electrokinetic systems, particularly under circumstances in which relatively weak ICP effects are observed using only a single channel.

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“…[4][5][6][7] One challenge is to detect the presence of a protein or a relevant biomolecule with detection strategies that commonly rely on immuno-or enzyme-based assays. 8 Additionally, with growing applications in genomics, proteomics, lipidomics, and analyses needed for emerging infectious diseases, a large number of sensors with and without labelling for protein sensing have also been reported, [9][10][11] with the use of ionic currents 12 and electrokinetic concentration polarization phenomena 13 in nanofluidics being oft-used methods to achieve large scale protein pre-concentrations. 14 For high yields and sensitivity, nearly all specific binding and detection strategies require either pre-concentration or concentration-amplification of target analytes.…”

Section: Introductionmentioning

confidence: 99%

Voltage-gated nanofluidic devices for protein capture, concentration, and release

Rangharajan

Prakash

2022

Analyst

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Nanoelectrokinetic Selective Preconcentration Based on Ion Concentration Polarization

Cited by 20 publications

References 88 publications

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

Electropreconcentration diagrams to optimize molecular enrichment with low counter pressure in a nanofluidic device

Predicting ion concentration polarization and analyte stacking/focusing at nanofluidic interfaces

Voltage-gated nanofluidic devices for protein capture, concentration, and release

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