Nanodiamonds for Medical Applications: Interaction with Blood in Vitro and in Vivo

Tsai, Lin-Wei; Lin, Ying‐Chih; Perevedentseva, E.; Луговцов, А.Е.; Priezzhev, Alexander V.; Cheng, Chia‐Liang

doi:10.3390/ijms17071111

Cited by 80 publications

(76 citation statements)

References 53 publications

(93 reference statements)

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“…46 However, an in vivo model demonstrated that ND can attach to the RBC membrane and circulate with blood for at least 30 minutes, implying that ND has potential applications in medicine. 25 In the present study, the shape and size of erythrocytes were preserved in the lGO and sGO groups, with only a slight increase in the size of the RBC nucleus in sGO. In both groups, we did not observe any adhesion of GO flakes to the RBC surface during examination of peripheral blood smears.…”

supporting

confidence: 39%

“…There have been several reports in the literature demonstrating the toxicity of different CNPs in various biological embryo models, such as chicken, [16][17][18][19] zebrafish, [20][21][22][23][24][25] and Xenopus laevis. 26,27 For example, carbon nanotubes inhibited angiogenesis of the chorioallantoic membrane in chicken embryos and caused death before day 12 of the incubation period.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Kurantowicz

Sawosz

Halik

et al. 2017

IJN

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In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 μg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

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supporting

confidence: 39%

Section: Introductionmentioning

confidence: 99%

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Kurantowicz

Sawosz

Halik

et al. 2017

IJN

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“…Other nanodiamond varieties have demonstrated satisfactory biocompatibility in vivo. 17 The nanodiamonds (700 nm diameter) used in this study have not yet been studied in biological systems. However, nanoparticles in general tend to have reduced toxicity with increased diameter.…”

Section: Discussionmentioning

confidence: 99%

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Gerstenhaber¹,

Barone²,

Marcinkiewicz³

et al. 2017

IJN

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The aim of this feasibility study was to test the ability of fluorescent nanodiamond particles (F-NDP) covalently conjugated with bitistatin (F-NDP-Bit) to detect vascular blood clots in vivo using extracorporeal near-infrared (NIR) imaging. Specifically, we compared NIR fluorescence properties of F-NDP with N-V (F-NDP NV ) and N-V-N color centers and sizes (100–10,000 nm). Optimal NIR fluorescence and tissue penetration across biological tissues (rat skin, porcine axillary veins, and skin) was obtained for F-NDP NV with a mean diameter of 700 nm. Intravital imaging (using in vivo imaging system [IVIS]) in vitro revealed that F-NDP NV -loaded glass capillaries could be detected across 6 mm of rat red-muscle barrier and 12 mm of porcine skin, which equals the average vertical distance of a human carotid artery bifurcation from the surface of the adjacent skin (14 mm). In vivo, feasibility was demonstrated in a rat model of occlusive blood clots generated using FeCl 3 in the carotid artery bifurcation. Following systemic infusions of F-NDP NV -Bit (3 or 15 mg/kg) via the external carotid artery or femoral vein (N=3), presence of the particles in the thrombi was confirmed both in situ via IVIS, and ex vivo via confocal imaging. The presence of F-NDP NV in the vascular clots was further confirmed by direct counting of fluorescent particles extracted from clots following tissue solubilization. Our data suggest that F-NDP NV -Bit associate with vascular blood clots, presumably by binding of F-NDP NV -Bit to activated platelets within the blood clot. We posit that F-NDP NV -Bit could serve as a noninvasive platform for identification of vascular thrombi using NIR energy monitored by an extracorporeal device.

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“…Raman spectroscopic analysis of RBC was performed according to methods described previously [16,17]. For Raman spectroscopic investigation of ND-berberine and berberine interaction with RBC, the Raman spectra of RBC determined by hemoglobin (Hb) were measured using a Raman spectrometer (T64000, Jobin Yvon, France) with 633 nm excitation wavelength laser.…”

Section: Cells Raman Measurements and Confocal Fluorescence Imagingmentioning

confidence: 99%

“…Functionality of proteins attached on ND (e.g. enzyme [11], hemoglobin [16,17], lipoproteins [12], different drugs [8][9][10][18][19][20]) has been investigated. Difference in mechanism of interaction of nanodiamond conjugates and pure drug with the target objects can modify the treatment [18], particularly, to overcome the chemoresistance of tumor [19,20].…”

Section: Introductionmentioning

confidence: 99%

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Lin¹,

Lin²,

Tsai³

et al. 2017

JBPE

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Abstract. The natural isoquinoline alkaloid berberine has been demonstrated for its significant activity against a variety of deceases caused by bacterial and parasite infections. Nanodiamond (ND) has also been proven to have great potential for drug delivery. In this work, the complex of ND with berberine is constructed through surface functionalized ND, aiming for drug delivery applications. Spectroscopic analysis was performed on the complex interaction with human lung alveolar carcinoma epithelial cell (A549) and red blood cell (RBC) in comparison with berberine in solution. The studied cells were treated with carboxylated ND, ND-berberine complex and berberine, respectively. Their distributions in cells structures are visualized using ND and berberine's fluorescence signals for detection. The distribution of ND and ND-berberine complex differs with the distribution of berberine introduced in solution form. The results show that the ND with attached drug molecules can act as a drug carrier allowing controllable localization of the drug into the cell. This result can have implication in drug delivery, release and delivery tracing applications. © 2017 Journal of Biomedical Photonics & Engineering.Keywords: Nanodiamond; Berberine; Bio-Applications; Medical Applications; Fluorescence; Surface Functionality; Imaging; Drug delivery; Nanobiotechnology. targeted nanoparticles as specific Helicobacter pylori eradication therapy: in vitro and in vivo study," Nanomedicine (Lond) 10(1), 57-71 (2015). 48. P. R. Vuddanda, A. Mishra, S. K. Singh, and S. Singh, "Development of polymeric nanoparticles with highly entrapped herbal hydrophilic drug using nanoprecipitation technique: an approach of quality by design," Pharm. Dev. Technol.

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Nanodiamonds for Medical Applications: Interaction with Blood in Vitro and in Vivo

Cited by 80 publications

References 53 publications

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II)

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

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