Abstract:Particles of diamond in the 5 to 100 nm size range, known as nanodiamond, have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but they allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-… Show more
“…We present the images using ‘CubeHelix’ [40], a colourmap that increases linearly in perceived brightness, thereby making it suitable for colour-blind readers and reproduction in greyscale media. All unprocessed imaging data is available at the DOI given in [41] along with descriptions of the file format generated by the custom software running our microscope and scripts for importing the data into Fiji. Figure 1.( a ) Cluster of NDs imaged with single-photon excitation confocal microscopy using 532 nm laser excitation.…”
Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.
“…We present the images using ‘CubeHelix’ [40], a colourmap that increases linearly in perceived brightness, thereby making it suitable for colour-blind readers and reproduction in greyscale media. All unprocessed imaging data is available at the DOI given in [41] along with descriptions of the file format generated by the custom software running our microscope and scripts for importing the data into Fiji. Figure 1.( a ) Cluster of NDs imaged with single-photon excitation confocal microscopy using 532 nm laser excitation.…”
Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.
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