Nanodiamonds coupled with plant bioactive metabolites: A nanotech approach for cancer therapy

Gismondi, Angelo; Reina, Giacomo; Orlanducci, S.; Mizzoni, Francesca; Terranova, Maria Letizia; Canini, Antonella

doi:10.1016/j.biomaterials.2014.10.057

Cited by 86 publications

(62 citation statements)

References 40 publications

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“…NDs (4-5 nm crystallite primary size) were purchased from the Federal Research and Production Center "ALTAI" (Russia) and processed following the strategy widely described by Gismondi et al 17 and in Reina et al 18 In particular, for the present experiments, plasma-reduced NDs, obtained as briefly described next, were used. The reduction of the purified DNDs by atomic H was accomplished in a purpose-designed plasma-assisted chemical vapor deposition reactor, where the gas phase was excited by a dual-mode microwave (MW)/radio frequency (RF) plasma.…”

Section: Nd Synthesis and Drug Loadingmentioning

confidence: 99%

“…The obtained light gray-colored powder was finally collected in acetone and dried at room temperature for 24 hours. The total amount of molecules directly adsorbed on the surfaces of ND samples was measured ex post by UV-vis spectroscopy, following the procedure described by Gismondi et al 17 As reported by Reina et al, 18 citropten essentially bound to NDs via low polar interactions (ie, p-p stacking), and the amount of the loaded drug was 3.2 µmol of C/mg of ND. In this work, naked and 5,7-dimethoxycoumarin-conjugated reduced DNDs are simply named ND and ND + C, respectively.…”

Section: Nd Synthesis and Drug Loadingmentioning

confidence: 99%

“…17 Citropten resuspended in dimethyl sulfoxide (DMSO; Sigma-Aldrich Co.), in order to avoid the solvent concentration exceeding 0.1% during the treatment, was used at the final concentration of 400 µM and 640 µM for 6 hours, 8 hours, 24 hours, 48 hours, and 72 hours. Control cells were treated with DMSO (control for citropten) or PBS (control for ND samples) at the same volume of the respective treatment.…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

“…The first of them illustrated the antimicrobial activity of menthol ((1R,2S,5R)-2-isopropyl-5-methylcyclohexanol)-modified ND particles. 16 The other ones, published by our research group, 17,18 reported the functionalization of three different types of DNDs with citropten (5,7-dimethoxycoumarin) or quercetin (3,3′,4′,5,6-pentahydroxyflavone), the chemical characterization of these adducts, and their antineoplastic effects on mammalian cancer cells. On the other hand, the association of DNDs with stilbenes (ie, resveratrol), a class of plant polyphenols, was recently proposed, but not yet realized, for the treatment of some pathologies, considering the great anti-radical and cardioprotective properties of these molecules.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the association of DNDs with stilbenes (ie, resveratrol), a class of plant polyphenols, was recently proposed, but not yet realized, for the treatment of some pathologies, considering the great anti-radical and cardioprotective properties of these molecules. 19 On the basis of our previous research, 17 in order to promote and support the possible biomedical use of plant drugs conjugated with NDs in cancer therapy, we investigated the exact molecular mechanism of action of DNDs coupled with citropten. Moreover, for the first time, we performed a study on citropten internalization kinetics and the evaluation of its concentration in mammalian cells after exposure to the pure or ND-functionalized drug.…”

Section: Introductionmentioning

confidence: 99%

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Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Gismondi¹,

Nanni²,

Reina³

et al. 2016

IJN

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For the first time, we coupled reduced detonation nanodiamonds (NDs) with a plant secondary metabolite, citropten (5,7-dimethoxycoumarin), and demonstrated how this complex was able to reduce B16F10 tumor cell growth more effectively than treatment with the pure molecule. These results encouraged us to find out the specific mechanism underlying this phenomenon. Internalization kinetics and quantification of citropten in cells after treatment with its pure or ND-conjugated form were measured, and it was revealed that the coupling between NDs and citropten was essential for the biological properties of the complex. We showed that the adduct was not able to induce apoptosis, senescence, or differentiation, but it determined cell cycle arrest, morphological changes, and alteration of mRNA levels of the cytoskeletal-related genes. The identification of metaphasic nuclei and irregular disposition of β-actin in the cell cytoplasm supported the hypothesis that citropten conjugated with NDs showed antimitotic properties in B16F10 cells. This work can be considered a pioneering piece of research that could promote and support the biomedical use of plant drug-functionalized NDs in cancer therapy

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Section: Nd Synthesis and Drug Loadingmentioning

confidence: 99%

Section: Nd Synthesis and Drug Loadingmentioning

confidence: 99%

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Gismondi¹,

Nanni²,

Reina³

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

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Multicombination Approach Suppresses Listeria monocytogenes‐Induced Septicemia‐Associated Acute Hepatic Failure: The Role of iRhom2 Signaling

Qin

et al. 2018

Adv Healthcare Materials

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The mortality rate of acute liver failure significantly increases due to fatal septicemia. Inactive rhomboid protein 2 (iRhom2) is an essential regulator of shedding TNF-α by trafficking with TNF-α converting enzyme (TACE). Fisetin, a flavonoid present in various fruits and plants, possesses anti-oxidative stress and anti-inflammatory activities. Here, multi-combination nanoparticles Fe@Au conjugated with fisetin, iRhom2 small interfering RNA (siRNA), and TNF-α inhibitor (FN) are prepared to examine their effects on fatal septicemia-associated hepatic failure induced by Listeria monocytogenes (LM) in mice and to reveal the underlying mechanisms. After LM infection, upregulation of glutamic-oxalacetic transaminease, glutamic-pyruvic transaminase, alkaline phosphatase, TNF-α, malondialdehyde, H O , and O is observedcompared to FN-treated mice. The iRhom2/TACE/TNF-α signals are enhanced in vivo and in vitro, resulting in oxidative stress, which is especially associated with the activation of kupffer cells and other macrophages. Decrease in Nrf2 activation and increase of inflammation-associated regulators are also noted in vivo and in vitro. Furthermore, overexpression of TNF-α derived from macrophages aggravates hepatic failure. Inversely, the processes above are restored by FN nanoparticles through the regulation of the iRhom2/TACE/TNF-α axis and Nrf2 activation. These findings suggest that FN may be a potential approach to protect against bacterial septicemia-related diseases by targeting iRhom2.

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Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead

et al. 2019

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Nanodiamond(ND)‐based technologies are flourishing in a wide variety of fields spanning from electronics and optics to biomedicine. NDs are considered a family of nanomaterials with an sp3 carbon core and a variety of sizes, shapes, and surfaces. They show interesting physicochemical properties such as hardness, stiffness, and chemical stability. Additionally, they can undergo ad‐hoc core and surface functionalization, which tailors them for the desired applications. Noteworthy, the properties of NDs and their surface chemistry are highly dependent on the synthetic method used to prepare them. In this Minireview, we describe the preparation of NDs from the materials‐chemistry viewpoint. The different methodologies of synthesis, purification, and surface functionalization as well as biomedical applications are critically discussed. New synthetic approaches as well as limits and obstacles of NDs are presented and analyzed.

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Nanodiamonds coupled with plant bioactive metabolites: A nanotech approach for cancer therapy

Cited by 86 publications

References 40 publications

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Multicombination Approach Suppresses Listeria monocytogenes‐Induced Septicemia‐Associated Acute Hepatic Failure: The Role of iRhom2 Signaling

Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead

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