Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection

Dória, Gonçalo; Franco, Ricardo; Baptista, Pedro V.

doi:10.1049/iet-nbt:20070001

Cited by 84 publications

(49 citation statements)

References 23 publications

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“…This approach was capable to detect less than 100 fmol/ml of the specific RNA target (less than 10 ng/ml of total RNA). Also, the non-cross-linking approach could distinguish common-base mismatch (SNPs) within the b-globin gene [35]. The authors could detect three different individual mutations using only one Au-nanoprobe.…”

Section: Aunps For Colorimetric Sensingmentioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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Section: Aunps For Colorimetric Sensingmentioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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“…It is a promising field as more and improved techniques are becoming available for clinical diagnostics with increased sensitivity at lower costs (Baptista et al, 2008;Baptista et al, 2005;Doria et al, 2007b;Baptista et al, 2006).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

“…In fact, AuNPs functionalized with ssDNA capable of specifically hybridizing to a complementary target for the detection of specific nucleic acid sequences in biological samples have been extensively used (Li and Rothberg, 2004;Mirkin et al, 1996;Cheng et al, 2006;Baptista et al, 2008;Doria et al, 2007b;Taton et al, 2000;Qin and Yung, 2007;Sato et al, 2005;Sato et al, 2003;Elghanian et al, 1997).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

“…This method has been successfully applied to detect eukaryotic gene expression without retro-transcription or PCR amplification steps (Conde et al, 2010b;Baptista et al, 2005); to distinguish fully complementary from mismatched sequences, with a single base mismatch i.e. to detect common mutations within the β-globin gene (Doria et al, 2007b); and in a fast and straightforward assay for Mycobacterium tuberculosis DNA detection in clinical samples (Costa et al, 2009;Baptista et al, 2006).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

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Multifunctional Gold Nanocarriers for Cancer Theranostics: From Bench to Bedside and Back Again?

Conde

Tian²,

Baptista

et al. 2014

Nano-Oncologicals

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After a quarter of century of rapid technological advances, research has revealed the complexity of cancer, a disease intimately related to the dynamic transformation of the genome. However, the full understanding of the molecular onset of this disease is still far from achieved and the search for mechanisms of treatment will follow closely.It is here that Nanotechnology enters the fray offering a wealth of tools to diagnose and treat cancer.It is indisputable that the use of gold nanocarriers has been gaining momentum as vectors for therapy and diagnostic strategies, combining the AuNPs' ease of functionalization with numerous biomolecules, high loading capacity and fast uptake by target cells. In fact, over the last decade nearly 12.000 research papers focusing on multifunctional gold nanocarriers have been published in peer-reviewed journals. Some of the described nanosystems will most likely revolutionize our understanding of biological mechanisms and push forward the clinical practice through their 2 integration in future diagnostics platforms. Nevertheless, very little gave fruitful results in order to improve a bench-to-bedside approach to translational research. On the basis of theoretical and experimental results obtained so far are we or not at the point: from bench to bedside and back again? As you will see, the answers to this question are complex, but one thing is clear: Translation into clinics is a tortuous and difficult path. Here, we provide a critical review about the available multifunctional gold nanocarriers for in vitro application and in vivo cancer targeting on nanodiagnostics and therapy.

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“…DNA or protein can be used as a linking molecule to aggregate the AuNPs and thus taking advantage of the optical properties of disperse versus aggregated gold particles for biodetection assays (Mirkin et al, 1996). Recently, a non-cross-linking hybridization method has been presented, where aggregation of the DNA-functionalized gold nanoparticles (Au-nanoprobes) is induced by an increasing ionic strength of the solution-the presence of a complementary target to the Au-nanoprobe prevents aggregation and the solution remains red; whereas the presence of non-complementary/mismatched targets does not prevent aggregation, resulting in a visible color change from red to blue (Baptista Doria et al, 2007). We have previously integrated this system with an optoelectronic platform, using a high intensity light source and a color sensitive amorphous/nanocrystalline silicon photodetector, so as to create a high efficiency biosensor that can be used for the detection of specific DNA sequences (Fortunato et al, 2006;Martins et al, 2007;Silva et al, 2008).…”

Section: Introductionmentioning

confidence: 99%