Nanodiagnostics: A review of the medical capabilities of nanopores

Lastra, Lauren S.; Sharma, Vinay; Farajpour, Nasim; Nguyen, Michelle; Freedman, Kevin J.

doi:10.1016/j.nano.2021.102425

Cited by 12 publications

(6 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the Au surface was modified with Hcy (homocysteine), which led to a significant change in the current-voltage characteristics, practically after the modification the translocation time of insulin through the pore increases, which is explained by hydrophobic interactions between the protein (insulin) and the Hcy layer [ 127 ]. The use of homocysteine-modified metal nanopores has been shown to be effective, as protein uptake on chemically modified nanopores slows the translocation process to tens of milliseconds [ 128 ]. Another approach to the analysis and subsequent sequencing of proteins is tried with the ONT method, which is now used for DNA sequencing.…”

Section: Methodological Approaches and Challengesmentioning

confidence: 99%

Detection of Biological Molecules Using Nanopore Sensing Techniques

et al. 2023

View full text Add to dashboard Cite

Modern biomedical sensing techniques have significantly increased in precision and accuracy due to new technologies that enable speed and that can be tailored to be highly specific for markers of a particular disease. Diagnosing early-stage conditions is paramount to treating serious diseases. Usually, in the early stages of the disease, the number of specific biomarkers is very low and sometimes difficult to detect using classical diagnostic methods. Among detection methods, biosensors are currently attracting significant interest in medicine, for advantages such as easy operation, speed, and portability, with additional benefits of low costs and repeated reliable results. Single-molecule sensors such as nanopores that can detect biomolecules at low concentrations have the potential to become clinically relevant. As such, several applications have been introduced in this field for the detection of blood markers, nucleic acids, or proteins. The use of nanopores has yet to reach maturity for standardization as diagnostic techniques, however, they promise enormous potential, as progress is made into stabilizing nanopore structures, enhancing chemistries, and improving data collection and bioinformatic analysis. This review offers a new perspective on current biomolecule sensing techniques, based on various types of nanopores, challenges, and approaches toward implementation in clinical settings.

show abstract

Section: Methodological Approaches and Challengesmentioning

confidence: 99%

Detection of Biological Molecules Using Nanopore Sensing Techniques

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Because of its high resolution and capacity to assess parameters including perfusion, viability, and fibrosis, it is an excellent candidate for adding nanotheranostics. Nanotheranostics based on CMR have the potential to improve diagnostic capabilities of CMR while also allowing for more precise therapeutic interventions . Nanoparticle-based contrast agents visualized using CMR are used in CMR-based nanotheranostics.…”

Section: Innovative Methodologies Of Nanotheranostics For Cvdsmentioning

confidence: 99%

“…Nanotheranostics based on CMR have the potential to improve diagnostic capabilities of CMR while also allowing for more precise therapeutic interventions. 86 Nanoparticle-based contrast agents visualized using CMR are used in CMR-based nanotheranostics. Therapeutic payloads such as medications, DNA, or heat-generating chemicals for hyperthermia treatments can be designed to be transported by these NPs.…”

Section: Cardiovascular Magneticmentioning

confidence: 99%

Current Advances in Nanotheranostics for Molecular Imaging and Therapy of Cardiovascular Disorders

Setia,

Mehata,

Priya

et al. 2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

“…Nanopores, especially the ss nanopores, allow for easy integration with conventional single molecule tools such as the vast pool of optical methods and microfluidic and optofluidic platforms, making them a general molecular analysis platform for a variety of other applications [14,101]. Recently, the optofluidic integration of nanopores has gained impetus toward developing economic, portable, chip-scale, single-molecule probing and diagnostic tools [416][417][418]. An interesting application of such integration is the simultaneous electro-optical detection of single molecules.…”

Section: Electrical Methods For Single-molecule Experimentsmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

View full text Add to dashboard Cite

Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

show abstract

Nanodiagnostics: A review of the medical capabilities of nanopores

Cited by 12 publications

References 107 publications

Detection of Biological Molecules Using Nanopore Sensing Techniques

Detection of Biological Molecules Using Nanopore Sensing Techniques

Current Advances in Nanotheranostics for Molecular Imaging and Therapy of Cardiovascular Disorders

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Contact Info

Product

Resources

About