Nanocurcumin alleviates insulin resistance and pancreatic deficits in polycystic ovary syndrome rats: Insights on PI3K/AkT/mTOR and TNF-α modulations

Abuelezz, Nermeen Z.; Shabana, Marwa E; Abdel-Mageed, Heidi M.; Rashed, Laila Ahmed; Morcos, George

doi:10.1016/j.lfs.2020.118003

Cited by 46 publications

(48 citation statements)

References 72 publications

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“… 19 , 52 Interestingly, we recently reported decreased PI3k/AKT/mTOR levels in PCOS. 12 Taken together, these results might resolve miR-223-3p as an essential jigsaw upregulator to the disturbed pathway and associated pathologies in PCOS pancreas.…”

Section: Discussionmentioning

confidence: 85%

“…In line with our findings, numerous studies have previously confirmed the disturbed metabolic profile and eruption of insulin in PCOS rat model, as well as clinically. 12 , 21 , 27 , 28 …”

Section: Discussionmentioning

confidence: 99%

“…Nanocurcumin was formulated according to the previously mentioned method. 12 Briefly, curcumin powder was dissolved in chloroform, and the solution was added dropwise to PVA solution with continuous stirring at 70°C. The temperature was gradually decreased to 40°C while stirring until a yellow fluffy mass was formed.…”

Section: Methodsmentioning

confidence: 99%

“… 27 , 28 Nanocurcumin doses (100 and 200 mg/kg) were chosen as the best tested doses based on the results of our previous study. 12 By the end of the experiment, the animals were fasted for 12 h, and then anaesthetized with isoflurane. Blood was collected via lateral retino orbital puncture, and serum was obtained by centrifugation (2000 × g for 15 min).…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies have reported an impaired PI3K/AKT/mTOR pathway in metabolic-related disorders, as this pathway regulates metabolic and proliferative processes across the body. 12 , 13 However, the upstream regulators that encounter this pathway to autophagy in PCOS pancreas are still unresolved. The crucial role of inflammatory cytokine nuclear factor-κB (NF-κB) in manipulating insulin activity and autophagy during inflammation is constantly emerging.…”

Section: Introductionmentioning

confidence: 99%

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Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Abuelezz¹,

Shabana²,

Rashed³

et al. 2021

JEP

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Purpose Polycystic ovary syndrome (PCOS) is a prevalent female endocrine disorder. 50–70% of PCOS patients suffer from glucose intolerance, insulin and β cell impairments. Updated studies reveal the crucial regulatory role of inflammation modulators in various diseases, by manipulating autophagy and oxidative stress. However, the data available about autophagy in PCOS pancreas, especially in relation to inflammation key players are little. This study investigated pancreatic autophagy status in PCOS rat model, with miR-223-3p and NF-κB levels as pivotal regulators of oxidative stress-autophagy axis, insulin, and β cell integrity. We then analyzed nanocurcumin effects as a putative anti-inflammatory nutraceutical on the disrupted parameters. Methods Nanocurcumin was characterized using transmission electron microscopy (TEM) and Fourier-transform IR (FT-IR) spectroscopy. Adult virgin Wistar rats were selected, and PCOS was induced using letrozole (1mg/kg). Nanocurcumin was ingested following letrozole. Sex hormones and insulin resistance were determined. miR-223-3p expression was determined using real-time PCR. Immunohistochemistry and Western blotting determined β cells, NF-κB, and autophagy markers p62 and LC3II. Results PCOS group showed significant disruptions in sex hormones and a double fold increase in glucose and insulin levels, exhibiting insulin resistance. Immunostaining confirmed around 46% deterioration of ß cell mass. Real-time PCR showed significant downregulation of miR-223-3p. Immunohistochemistry and Western blotting revealed a drastic upsurge of NF-κB, and autophagy markers p62 and LC3II, confirming bioinformatics target analysis. Interestingly, compared to PCOS group, nanocurcumin (200mg/kg) significantly upregulated miR-223-3p expression by 30%. It subsided NF-κB and autophagy eruption to restore ß cell mass and attenuate insulin resistance. Conclusion To the best of our knowledge, this study is the first to highlight the vital contribution of miR-223-3p and NF-κB levels in aggravating PCOS pancreatic autophagy and consequent impairments. It spots nanocurcumin potential as an inflammation and autophagy modulator, for possible better management of PCOS complications.

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Section: Discussionmentioning

confidence: 85%

“…In line with our findings, numerous studies have previously confirmed the disturbed metabolic profile and eruption of insulin in PCOS rat model, as well as clinically. 12 , 21 , 27 , 28 …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Abuelezz¹,

Shabana²,

Rashed³

et al. 2021

JEP

Self Cite

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Multifaceted physiological and therapeutical impact of curcumin on hormone‐related endocrine dysfunctions: A comprehensive review

Wang,

Zhang,

Zhou

2024

Phytotherapy Research

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Over the past five decades, Curcumin (Cur), derived from turmeric (Curcuma longa), has gained considerable attention for its potential therapeutic applications. Synthesizing insights from clinical trials conducted over the last 25 years, this review delves into diseases where Cur has demonstrated promise, offering a nuanced understanding of its pharmacokinetics, safety, and effectiveness. Focusing on specific examples, the impact of Cur on various human diseases is explored. Endocrine glands and associated signaling pathways are highlighted, elucidating how Cur influences cellular signaling. The article underscores molecular mechanisms such as hormone level alteration, receptor interaction, cytokine and adipokine expression inhibition, antioxidant enzyme activity, and modulation of transcription factors. Cur showcases diverse protective mechanisms against inflammation and oxidative damage by suppressing antiapoptotic genes and impeding tumor promotion. This comprehensive overview emphasizes the potential of Cur as a natural agent for countering aging and degenerative diseases, calling for further dedicated research in this realm.

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The Application of Nanoparticle‐Based Imaging and Phototherapy for Female Reproductive Organs Diseases

Luo

Zhou

Wang

et al. 2023

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Various female reproductive disorders affect millions of women worldwide and bring many troubles to women's daily life. Let alone, gynecological cancer (such as ovarian cancer and cervical cancer) is a severe threat to most women's lives. Endometriosis, pelvic inflammatory disease, and other chronic diseases‐induced pain have significantly harmed women's physical and mental health. Despite recent advances in the female reproductive field, the existing challenges are still enormous such as personalization of disease, difficulty in diagnosing early cancers, antibiotic resistance in infectious diseases, etc. To confront such challenges, nanoparticle‐based imaging tools and phototherapies that offer minimally invasive detection and treatment of reproductive tract‐associated pathologies are indispensable and innovative. Of late, several clinical trials have also been conducted using nanoparticles for the early detection of female reproductive tract infections and cancers, targeted drug delivery, and cellular therapeutics. However, these nanoparticle trials are still nascent due to the body's delicate and complex female reproductive system. The present review comprehensively focuses on emerging nanoparticle‐based imaging and phototherapies applications, which hold enormous promise for improved early diagnosis and effective treatments of various female reproductive organ diseases.

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Nanocurcumin alleviates insulin resistance and pancreatic deficits in polycystic ovary syndrome rats: Insights on PI3K/AkT/mTOR and TNF-α modulations

Cited by 46 publications

References 72 publications

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Multifaceted physiological and therapeutical impact of curcumin on hormone‐related endocrine dysfunctions: A comprehensive review

The Application of Nanoparticle‐Based Imaging and Phototherapy for Female Reproductive Organs Diseases

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