Nanocrystalline diamond surfaces for adhesion and growth of primary neurons, conflicting results and rational explanation

Ojovan, Silviya M.; McDonald, Matthew; Rabieh, Noha; Nava, Stefano; Erez, Hadas; Nesládek, Miloš; Spira, Micha Ε.

doi:10.3389/fneng.2014.00017

Cited by 20 publications

(17 citation statements)

References 26 publications

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“…In these cases the IN-CELL recording configuration was stable for at least the duration of the recording session which lasted 30–45 min. As previously shown and discussed, IN-CELL recordings are the outcome of a combination of factors including the seal resistance formed by the engulfment of a gMμE by a cell, the junctional membrane conductance (the patch of membrane that faces the gMμE) of the cell, and the electrode impedance143132333563. Although spontaneous IN-CELL recordings of myotubes are rare it is conceivable that methods to facilitate their spontaneous formation can be developed.…”

Section: Discussionmentioning

confidence: 99%

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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In contrast to the extensive use of microelectrode array (MEA) technology in electrophysiological studies of cultured neurons and cardiac muscles, the vast field of skeletal muscle research has yet to adopt the technology. Here we demonstrate an empowering MEA technology for high quality, multisite, long-term electrophysiological recordings from cultured skeletal myotubes. Individual rat skeletal myotubes cultured on micrometer sized gold mushroom-shaped microelectrode (gMμE) based MEA tightly engulf the gMμEs, forming a high seal resistance between the myotubes and the gMμEs. As a consequence, spontaneous action potentials generated by the contracting myotubes are recorded as extracellular field potentials with amplitudes of up to 10 mV for over 14 days. Application of a 10 ms, 0.5–0.9 V voltage pulse through the gMμEs electroporated the myotube membrane, and transiently converted the extracellular to intracellular recording mode for 10–30 min. In a fraction of the cultures stable attenuated intracellular recordings were spontaneously produced. In these cases or after electroporation, subthreshold spontaneous potentials were also recorded. The introduction of the gMμE-MEA as a simple-to-use, high-quality electrophysiological tool together with the progress made in the use of cultured human myotubes opens up new venues for basic and clinical skeletal muscle research, preclinical drug screening, and personalized medicine.

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Section: Discussionmentioning

confidence: 99%

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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“…This is a common undesired side effect of the fixation-dehydration procedure and was observed, irrespective of cell density and peptide concentration. Compared to previous work conducted by other research groups, our neuron cultures appear healthy with a well-developed neurite network [40][41][42][43][44]. However, as the dimensions of the PNWs are of the same order of magnitude as the neurites, it is difficult to distinguish the full extent of the network (Fig.…”

Section: Resultsmentioning

confidence: 57%

“…In parallel, neurons plated on poly-D-lysine-coated polystyrene in 2D served as control. As various studies show that cells thrive better on non-flat substrates [7,19,[40][41][42][43][44], we hypothesize that neocortical neurons cultured on PNWs are functionally and metabolically superior to neurons cultured the traditional way in 2D.…”

Section: Introductionmentioning

confidence: 91%

Diphenylalanine Peptide Nanowires as a Substrate for Neural Cultures

et al. 2019

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Primary brain cells cultured on flat surfaces, i.e., in a two-dimensional fashion, have a long history of use as an experimental model system in neuroscience research. However, it is questionable to which extent these cultured brain cells resemble their in vivo counterparts. Mainly, it has been claimed that the non-oxidative glucose metabolism reflected by lactate production is unphysiologically high. Furthermore, it is known that culturing in 2D alters the phenotype of cells. Here we present diphenylalanine peptide nanowires (PNWs) as a culturing substrate for primary neocortical neurons from mice. The topology of the PNWs leads to neuronal cultures developing in 2.5D environment and hence improved culturing conditions. We investigate the effect of different concentrations of PNWs and different cell densities of neurons on the culturing conditions. The neocortical neurons were examined through scanning electron microscopy in order to study the effect of PNW concentrations and neuron densities on the structural appearance of the cells. Then employing the optimal combination of neuron density and PNW concentration, the neurons were evaluated functionally and metabolically by comparison with neocortical neurons standard culturing methods in 2D. Specifically, we tested neuronal viability, capacity for vesicular release of neurotransmitter GABA, as well as oxidative and non-oxidative glucose metabolism. It was evident that neurons cultured on PNWs exhibited increased viability combined with an increased capacity for neurotransmitter release and a lower fraction of non-oxidative metabolism than neurons cultured in 2D. Hence, neocortical neurons cultured in 2.5D on PNWs appear to be healthier and less glycolytic than neurons cultured in 2D.

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“…Even though many scientific groups are working on this task, the concept of a diamond as a neuroelectrode is still new and some of the fundamental questions remain open. As an example, only recently (and after more than 10 years after first articles appeared on this topic) it was rationally explained why primary neuron cultures fail to grow on the bare diamond surface 26 . Optimisation of neural adhesion to the electrode surface is of major importance for assessment of neural cells and tissues in vitro, such as microelectrodearrays (MEAs).…”

Section: Introductionmentioning

confidence: 99%

Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

et al. 2018

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The ability to form an efficient interface between material and neural cells is a crucial aspect of the construction of neuroelectrodes. Diamond offers material characteristics that could, by a large extent, improve the performance of neuroelectrodes. The greatest advantage of diamond is a large variety of material and surface properties such as electrical conductivity, surface morphology, and surface chemistry. Such a variety of material characteristics can lead to various cellular responses. Here we compare survival, adhesion, and neurite formation of primary neurons on diamond thin films of various morphology and on their treatment with several types of polymers commonly used to enhance cell adhesion. We found that the variation of surface roughness of nanocrystalline diamond film does not have a major influence on the neuron survival or adhesion. The adhesion of neurons can beinfluenced by the selected type of polymer coating.

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Nanocrystalline diamond surfaces for adhesion and growth of primary neurons, conflicting results and rational explanation

Cited by 20 publications

References 26 publications

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Diphenylalanine Peptide Nanowires as a Substrate for Neural Cultures

Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

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